Solution nuclear magnetic resonance (NMR) spectroscopy and, in particular, chemical shift perturbation (CSP) titration experiments are ideally suited for mapping and characterizing the binding interface of macromolecular complexes. ¹H-^(15)N-HSQC-based CSP studies have become the method of choice due to their simplicity, short-time requirements, and minimal working knowledge of NMR. CSP studies for characterizing protein-glycosaminoglycan (GAG) interactions can be challenging due to...
[NMR paper] Characterizing Protein-Protein Interactions Using Solution NMR Spectroscopy.
Characterizing Protein-Protein Interactions Using Solution NMR Spectroscopy.
Characterizing Protein-Protein Interactions Using Solution NMR Spectroscopy.
Methods Mol Biol. 2018;1764:73-85
Authors: Ortega-Roldan JL, Blackledge M, Jensen MR
Abstract
In this chapter, we describe how NMR chemical shift titrations can be used to study the interaction between two proteins with emphasis on mapping the interface of the complex and determining the binding affinity from a quantitative analysis of the experimental data. In particular, we...
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04-02-2018 03:36 PM
[NMR paper] Investigating protein-ligand interactions by solution NMR spectroscopy.
Investigating protein-ligand interactions by solution NMR spectroscopy.
Investigating protein-ligand interactions by solution NMR spectroscopy.
Chemphyschem. 2018 Jan 04;:
Authors: Becker W, Bhattiprolu KC, Gubensäk N, Zangger K
Abstract
Protein-ligand interactions are of fundamental importance in almost all processes in living organisms. The ligands comprise small molecules, drugs or biological macromolecules and their interaction strength varies over several orders of magnitude. Solution NMR spectroscopy offers a large...
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01-10-2018 12:45 PM
Characterizing Substrate-Surface Interactions on Alumina-Supported Metal Catalysts by Dynamic Nuclear Polarization-Enhanced Double-Resonance NMR Spectroscopy #DNPNMR
From The DNP-NMR Blog:
Characterizing Substrate-Surface Interactions on Alumina-Supported Metal Catalysts by Dynamic Nuclear Polarization-Enhanced Double-Resonance NMR Spectroscopy #DNPNMR
p.p1 {margin: 0.0px 0.0px 0.0px 36.0px; text-indent: -36.0px; font: 12.0px Helvetica}
Perras, F.A., et al., Characterizing Substrate-Surface Interactions on Alumina-Supported Metal Catalysts by Dynamic Nuclear Polarization-Enhanced Double-Resonance NMR Spectroscopy. J Am Chem Soc, 2017. 139(7): p. 2702-2709.
https://www.ncbi.nlm.nih.gov/pubmed/28112506
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06-13-2017 06:55 AM
[NMR paper] (19)F labelled glycosaminoglycan probes for solution NMR and non-linear (CARS) microscopy.
(19)F labelled glycosaminoglycan probes for solution NMR and non-linear (CARS) microscopy.
Related Articles (19)F labelled glycosaminoglycan probes for solution NMR and non-linear (CARS) microscopy.
Glycoconj J. 2016 Aug 15;
Authors: Lima MA, Cavalheiro RP, M Viana G, Meneghetti MC, Rudd TR, Skidmore MA, Powell AK, Yates EA
Abstract
Studying polysaccharide-protein interactions under physiological conditions by conventional techniques is challenging. Ideally, macromolecules could be followed by both in vitro spectroscopy...
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08-16-2016 07:38 PM
[NMR paper] Characterizing protein-glycosaminoglycan interactions using solution NMR spectroscopy.
Characterizing protein-glycosaminoglycan interactions using solution NMR spectroscopy.
Characterizing protein-glycosaminoglycan interactions using solution NMR spectroscopy.
Methods Mol Biol. 2015;1229:325-33
Authors: Joseph PR, Poluri KM, Sepuru KM, Rajarathnam K
Abstract
Solution nuclear magnetic resonance (NMR) spectroscopy and, in particular, chemical shift perturbation (CSP) titration experiments are ideally suited for characterizing the binding interface of macromolecular complexes. (1)H-(15) N-HSQC-based CSP studies have...
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10-19-2014 01:27 PM
[NMR paper] Characterizing carbohydrate-protein interactions by NMR.
Characterizing carbohydrate-protein interactions by NMR.
Characterizing carbohydrate-protein interactions by NMR.
Biopolymers. 2013 Jun 20;
Authors: Bewley CA, Shahzad-Ul-Hussan S
Abstract
Interactions between proteins and soluble carbohydrates and/or surface displayed glycans are central to countless recognition, attachment and signaling events in biology. The physical chemical features associated with these binding events vary considerably, depending on the biological system of interest. For example, carbohydrate-protein...
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06-21-2013 01:10 PM
[NMR paper] Characterizing RNA dynamics at atomic resolution using solution-state NMR spectroscopy.
From Mendeley Biomolecular NMR group:
Characterizing RNA dynamics at atomic resolution using solution-state NMR spectroscopy.
Nature methods (2011). Volume: 8, Issue: 11. Pages: 919-31. Jameson R Bothe, Evgenia N Nikolova, Catherine D Eichhorn, Jeetender Chugh, Alexandar L Hansen, Hashim M Al-Hashimi et al.
Many recently discovered noncoding RNAs do not fold into a single native conformation but sample many different conformations along their free-energy landscape to carry out their biological function. Here we review solution-state NMR techniques that measure the structural,...
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10-12-2012 09:58 AM
[NMR paper] Characterizing RNA dynamics at atomic resolution using solution-state NMR spectroscopy.
From Mendeley Biomolecular NMR group:
Characterizing RNA dynamics at atomic resolution using solution-state NMR spectroscopy.
Nature methods (2011). Volume: 8, Issue: 11. Pages: 919-31. Jameson R Bothe, Evgenia N Nikolova, Catherine D Eichhorn, Jeetender Chugh, Alexandar L Hansen, Hashim M Al-Hashimi et al.
Many recently discovered noncoding RNAs do not fold into a single native conformation but sample many different conformations along their free-energy landscape to carry out their biological function. Here we review solution-state NMR techniques that measure the structural,...
Solution NMR Spectroscopy for Characterizing Protein-Glycosaminoglycan Interactions
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