Related ArticlesSolution NMR conformation of glycosaminoglycans.
Prog Biophys Mol Biol. 2014 Feb 19;
Authors: Pomin VH
Abstract
Nuclear magnetic resonance (NMR) spectroscopy has been giving a pivotal contribution to the progress of glycomics, mostly by elucidating the structural, dynamical, conformational and intermolecular binding aspects of carbohydrates. Particularly in the field of conformation, NOE resonances, scalar couplings, residual dipolar couplings, and chemical shift anisotropy offsets have been the principal NMR parameters utilized. Molecular dynamics calculations restrained by NMR-data input are usually employed in conjunction to generate glycosidic bond dihedral angles. Glycosaminoglycans (GAGs) are a special class of sulfated polysaccharides extensively studied worldwide. Besides regulating innumerous physiological processes, these glycans are also widely explored in the global market as either clinical or nutraceutical agents. The conformational aspects of GAGs are key regulators to the quality of interactions with the functional proteins involved in biological events. This report discusses the solution conformation of each GAG type analyzed by one or more of the above-mentioned methods. This type of document is highly demanded nowadays considering the growing relevance of both NMR spectroscopy and GAGs to the current glycomic age.
PMID: 24560989 [PubMed - as supplied by publisher]
[NMR paper] Investigation of Lysine Side Chain Interactions of Interleukin-8 with Heparin and other Glycosaminoglycans Studied by a Methylation-NMR Approach.
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Glycobiology. 2013 Aug 27;
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Abstract
Although the interaction between interleukin-8 (IL-8) and glycosaminoglycans (GAG) is...
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NMR studies of the solution conformation of the sex peptide from Drosophila melanogaster.
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FEBS Lett. 2011 Apr 20;585(8):1197-202
Authors: Moehle K, Freund A, Kubli E, Robinson JA
The insect sex peptide (SP) elicits a variety of biological responses upon transfer to the mated female. SP contains 36 amino acids, including a tryptophan-rich N-terminal region, a central region containing five hydroxyproline (Hyp) residues, and a C-terminal region enclosed by a...
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[NMR paper] Active conformations of glycosaminoglycans. NMR determination of the conformation of
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Semin Thromb Hemost. 2002 Aug;28(4):325-34
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Binding to proteins usually induces perturbation of nuclear magnetic...
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[NMR paper] What is the average conformation of bacteriophage T4 lysozyme in solution? A domain o
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Lysozyme from T4 bacteriophage is comprised of two domains that are both involved in binding substrate. Although wild-type lysozyme has...
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Eur J Biochem. 1996 Jun...
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Kedarcidin is a recently discovered antitumor antibiotic chromoprotein. The solution conformation of the kedarcidin apoprotein (114 residues) has been characterized by heteronuclear...
Solution NMR conformation of glycosaminoglycans.
Linear Mode
