[NMR paper] The soluble Y115E-Y117E variant of human glutaminyl cyclase is a valid target for X-ray and NMR screening of inhibitors against Alzheimer disease.
Related ArticlesThe soluble Y115E-Y117E variant of human glutaminyl cyclase is a valid target for X-ray and NMR screening of inhibitors against Alzheimer disease.
Acta Crystallogr F Struct Biol Commun. 2015 Aug 1;71(Pt 8):986-92
Authors: DiPisa F, Pozzi C, Benvenuti M, Andreini M, Marconi G, Mangani S
Abstract
Recent developments in molecular pathology and genetics have allowed the identification of human glutaminyl cyclase (hQC) among the abnormal proteins involved in many neurodegenerative disorders. Difficulties in obtaining large quantities of pure protein may limit the use of crystallographic screening for drug development on this target. Site-directed mutagenesis experiments have led to the identification of some solvent-exposed residues that are absolutely critical to achieve increased solubility and to avoid precipitation of the enzyme in inclusion bodies when expressed in Escherichia coli. The designed variant Y115E-Y117E has been found to be able to provide large amounts of monodisperse, pure hQC from an E. coli expression system. To validate the use of the artificial construct as a target for large-scale X-ray and NMR screening campaigns in the search for new inhibitors of hQC, the X-ray crystal structures of the hQC Y115E-Y117E variant and of its adduct with the inhibitor PBD-150 were determined.
Plasma protein biomarkers of Alzheimer's disease endophenotypes in ... - Nature.com
Plasma protein biomarkers of Alzheimer's disease endophenotypes in ... - Nature.com
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Plasma protein biomarkers of Alzheimer's disease endophenotypes in ...
Nature.com
Volumes of the hippocampi and entorhinal cortices in the ANM cohort were obtained using FreeSurfer 5.1.0 (freely available from http://surfer.nmr.mgh.harvard.edu/) from 276 ANM subjects who had undergone structural MRI. These regions were selected as ...
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06-17-2015 08:01 AM
Plasma protein biomarkers of Alzheimer's disease endophenotypes in ... - Nature.com
Plasma protein biomarkers of Alzheimer's disease endophenotypes in ... - Nature.com
<img alt="" height="1" width="1">
Plasma protein biomarkers of Alzheimer's disease endophenotypes in ...
Nature.com
Volumes of the hippocampi and entorhinal cortices in the ANM cohort were obtained using FreeSurfer 5.1.0 (freely available from http://surfer.nmr.mgh.harvard.edu/) from 276 ANM subjects who had undergone structural MRI. These regions were selected as ...
Read here
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06-16-2015 07:24 PM
[NMR paper] (1)H- and (13)C-NMR spectroscopy of Thy-1-APPSL mice brain extracts indicates metabolic changes in Alzheimer's disease.
(1)H- and (13)C-NMR spectroscopy of Thy-1-APPSL mice brain extracts indicates metabolic changes in Alzheimer's disease.
Related Articles (1)H- and (13)C-NMR spectroscopy of Thy-1-APPSL mice brain extracts indicates metabolic changes in Alzheimer's disease.
J Neural Transm. 2015 Mar 6;
Authors: Doert A, Pilatus U, Zanella F, Müller WE, Eckert GP
Abstract
Biochemical alterations underlying the symptoms and pathomechanisms of Alzheimer's disease (AD) are not fully understood. However, alterations of glucose metabolism and...
Alzheimer's disease: NMR pinpoints genetic clue
Alzheimer's disease: NMR pinpoints genetic clue
http://www.spectroscopynow.com/common/images/thumbnails/1438cadfde3.jpgResearchers have turned to solution NMR spectroscopy to help them characterise the structure and dynamics of the transmembrane portion of the protein amyloid precursor protein, APP, of which there are two genetic variants linked to familial, or hereditary Alzheimer's disease.
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01-15-2014 05:05 AM
Alzheimer's analysed: New clues in brain disease
Alzheimer's analysed: New clues in brain disease
http://www.spectroscopynow.com/common/images/thumbnails/13af4d7f690.jpgRecent studies have added weight to the idea that amyloid-beta is a causative agent in Alzheimer's disease rather than the plaques formed from these chunks of misfolded peptide that deposit in the diseased brain. Now, European researchers have used REAPDOR solid-state NMR spectroscopy to analyse the hydrogen bonds in the tiny fibrils of amyloid-beta peptide, offering another perspective on this neurodegenerative disorder.
Source: Spectroscopynow.com
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02-03-2013 08:49 AM
[NMR paper] Alzheimer's disease: NMR studies of asialo (GM1) and trisialo (GT1b) ganglioside inte
Alzheimer's disease: NMR studies of asialo (GM1) and trisialo (GT1b) ganglioside interactions with Abeta(1-40) peptide in a membrane mimic environment.
Related Articles Alzheimer's disease: NMR studies of asialo (GM1) and trisialo (GT1b) ganglioside interactions with Abeta(1-40) peptide in a membrane mimic environment.
Neurochem Res. 2004 Feb;29(2):447-53
Authors: Mandal PK, Pettegrew JW
Amyloid peptide (Abeta) is the major protein constituent of neuritic plaques in Alzheimer's disease (AD). This peptide is an amphipathic molecule that...
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11-24-2010 09:25 PM
[NMR paper] Application of NMR SHAPES screening to an RNA target.
Application of NMR SHAPES screening to an RNA target.
Related Articles Application of NMR SHAPES screening to an RNA target.
J Am Chem Soc. 2003 Dec 24;125(51):15724-5
Authors: Johnson EC, Feher VA, Peng JW, Moore JM, Williamson JR
Several NMR screening techniques have been developed in recent years to aid in the identification of lead drug compounds. These NMR methods have traditionally been used for protein targets, and here we examine their applicability for an RNA target. We used the SHAPES compound library to test three different NMR...