Solid-StateNMR Provides Evidence for Small-AmplitudeSlow Domain Motions in a Multispanning Transmembrane ?-HelicalProtein

		BioNMR > Educational resources > Journal club
Solid-StateNMR Provides Evidence for Small-AmplitudeSlow Domain Motions in a Multispanning Transmembrane ?-HelicalProtein

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

07-01-2017, 11:41 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,732

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Solid-StateNMR Provides Evidence for Small-AmplitudeSlow Domain Motions in a Multispanning Transmembrane ?-HelicalProtein

Solid-StateNMR Provides Evidence for Small-AmplitudeSlow Domain Motions in a Multispanning Transmembrane ?-HelicalProtein

Daryl Good, Charlie Pham, Jacob Jagas, Jo?zef R. Lewandowski and Vladimir Ladizhansky

Journal of the American Chemical Society
DOI: 10.1021/jacs.7b03974

Source: Journal of the American Chemical Society

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] Solid-state NMR provides evidence for small-amplitude slow domain motions in a multi-spanning transmembrane ?-helical protein. Solid-state NMR provides evidence for small-amplitude slow domain motions in a multi-spanning transmembrane ?-helical protein. Solid-state NMR provides evidence for small-amplitude slow domain motions in a multi-spanning transmembrane ?-helical protein. J Am Chem Soc. 2017 Jun 14; Authors: Good D, Pham C, Jagas J, Lewandowski JR, Ladizhansky V Abstract Proteins are dynamic entities and populate ensembles of conformations. Transitions between states within a conformational ensemble occur over a broad spectrum of amplitude...	nmrlearner	Journal club	0	06-15-2017 03:37 PM
[NMR paper] Ultraslow Domain Motions in HIV-1 TAR RNA Revealed by Solid-State Deuterium NMR. Ultraslow Domain Motions in HIV-1 TAR RNA Revealed by Solid-State Deuterium NMR. Ultraslow Domain Motions in HIV-1 TAR RNA Revealed by Solid-State Deuterium NMR. J Phys Chem B. 2016 Dec 08; Authors: Huang W, Emani PS, Varani G, Drobny GP Abstract Intrinsic motions may allow HIV-1 TAR RNA to change its conformation to form a functional complex with the Tat protein, which is essential for viral replication. Understanding the dynamic properties of TAR necessitates determining motion on the intermediate ns-?s time scale. To...	nmrlearner	Journal club	0	12-09-2016 06:09 PM
ConformationalDynamics of a Seven Transmembrane HelicalProtein Anabaena Sensory RhodopsinProbed by Solid-State NMR ConformationalDynamics of a Seven Transmembrane HelicalProtein Anabaena Sensory RhodopsinProbed by Solid-State NMR Daryl B. Good, Shenlin Wang, Meaghan E. Ward, Jochem Struppe, Leonid S. Brown, Jo?zef R. Lewandowski and Vladimir Ladizhansky http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja411633w/aop/images/medium/ja-2013-11633w_0011.gif Journal of the American Chemical Society DOI: 10.1021/ja411633w http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/jacsat/~4/MTq1JhQmGFU	nmrlearner	Journal club	0	02-05-2014 06:08 PM
Evidence from solid-state NMR for nonhelical conformations in the transmembrane domain of the amyloid precursor protein. Evidence from solid-state NMR for nonhelical conformations in the transmembrane domain of the amyloid precursor protein. Evidence from solid-state NMR for nonhelical conformations in the transmembrane domain of the amyloid precursor protein. Biophys J. 2011 Feb 2;100(3):711-9 Authors: Lu JX, Yau WM, Tycko R The amyloid precursor protein (APP) is subject to proteolytic processing by ?-secretase within neuronal membranes, leading to Alzheimer's disease-associated ?-amyloid peptide production by cleavage near the midpoint of the*single...	nmrlearner	Journal club	0	02-02-2011 12:40 PM
[NMR paper] 15N and 31P solid-state NMR study of transmembrane domain alignment of M2 protein of 15N and 31P solid-state NMR study of transmembrane domain alignment of M2 protein of influenza A virus in hydrated cylindrical lipid bilayers confined to anodic aluminum oxide nanopores. Related Articles 15N and 31P solid-state NMR study of transmembrane domain alignment of M2 protein of influenza A virus in hydrated cylindrical lipid bilayers confined to anodic aluminum oxide nanopores. J Magn Reson. 2005 Apr;173(2):322-7 Authors: Chekmenev EY, Hu J, Gor'kov PL, Brey WW, Cross TA, Ruuge A, Smirnov AI This communication reports the first...	nmrlearner	Journal club	0	11-25-2010 08:21 PM
[NMR paper] Transmembrane domain of M2 protein from influenza A virus studied by solid-state (15) Transmembrane domain of M2 protein from influenza A virus studied by solid-state (15)N polarization inversion spin exchange at magic angle NMR. Related Articles Transmembrane domain of M2 protein from influenza A virus studied by solid-state (15)N polarization inversion spin exchange at magic angle NMR. Biophys J. 2000 Aug;79(2):767-75 Authors: Song Z, Kovacs FA, Wang J, Denny JK, Shekar SC, Quine JR, Cross TA The M2 protein from the influenza A virus forms a proton channel in the virion that is essential for infection. This tetrameric protein...	nmrlearner	Journal club	0	11-19-2010 08:29 PM
[NMR paper] NMR evidence for slow collective motions in cyanometmyoglobin. NMR evidence for slow collective motions in cyanometmyoglobin. Related Articles NMR evidence for slow collective motions in cyanometmyoglobin. Nat Struct Biol. 1997 Apr;4(4):292-7 Authors: Tolman JR, Flanagan JM, Kennedy MA, Prestegard JH Residual dipolar couplings observed in NMR spectra at very high magnetic fields have been measured to a high degree of accuracy for the paramagnetic protein cyanometmyoglobin. Deviations of these measurements from predictions based on available crystallographic and solution structures are largely systematic...	nmrlearner	Journal club	0	08-22-2010 03:31 PM
[NMR paper] NMR evidence for slow collective motions in cyanometmyoglobin. NMR evidence for slow collective motions in cyanometmyoglobin. Related Articles NMR evidence for slow collective motions in cyanometmyoglobin. Nat Struct Biol. 1997 Apr;4(4):292-7 Authors: Tolman JR, Flanagan JM, Kennedy MA, Prestegard JH Residual dipolar couplings observed in NMR spectra at very high magnetic fields have been measured to a high degree of accuracy for the paramagnetic protein cyanometmyoglobin. Deviations of these measurements from predictions based on available crystallographic and solution structures are largely systematic...	nmrlearner	Journal club	0	08-22-2010 03:03 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off