NMR paper Solid-state REDOR NMR distance measurements at the ligand site of a bacterial chemota

		BioNMR > Educational resources > Journal club
[NMR paper] Solid-state REDOR NMR distance measurements at the ligand site of a bacterial chemota

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

08-22-2010, 03:31 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Solid-state REDOR NMR distance measurements at the ligand site of a bacterial chemota

Solid-state REDOR NMR distance measurements at the ligand site of a bacterial chemotaxis membrane receptor.

Related Articles Solid-state REDOR NMR distance measurements at the ligand site of a bacterial chemotaxis membrane receptor.

Biochemistry. 1997 Feb 18;36(7):1699-703

Authors: Wang J, Balazs YS, Thompson LK

The Escherichia coli serine receptor senses serine levels in the environment and transmits this information across the bacterial inner membrane to modulate a protein phosphorylation cascade which controls swimming behavior. Solid-state nuclear magnetic resonance (NMR) has been used to characterize specific structural features of the ligand binding site interactions in the intact, membrane-bound Ser receptor. Rotational-echo double-resonance (REDOR) experiments on [15N]Ser bound to a [1-13C]Phe-receptor preparation are used to measure distances between the ligand amino group and the carbonyls of two phenylalanine residues in the ligand binding pocket. The results indicate two 4.0 +/- 0.2 A distances, in excellent agreement with the X-ray crystal structure of a soluble fragment of the homologous aspartate receptor [Milburn et al. (1991) Science 254, 1342-1347]. These results confirm the similarity of the binding sites of the Asp and Ser receptors, and demonstrate the feasibility of using solid-state NMR measurements to obtain specific structural information on the 120 kDa intact receptor for probing transmembrane signaling mechanisms.

PMID: 9048553 [PubMed - indexed for MEDLINE]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Interactions of protein side chains with RNA defined with REDOR solid state NMR. Interactions of protein side chains with RNA defined with REDOR solid state NMR. Interactions of protein side chains with RNA defined with REDOR solid state NMR. J Biomol NMR. 2011 Sep 25; Authors: Huang W, Varani G, Drobny GP Abstract Formation of the complex between human immunodeficiency virus type-1 Tat protein and the transactivation response region (TAR) RNA is vital for transcriptional elongation, yet the structure of the Tat-TAR complex remains to be established. The NMR structures of free TAR, and TAR bound to Tat-derived...	nmrlearner	Journal club	0	09-30-2011 06:00 AM
Interactions of protein side chains with RNA defined with REDOR solid state NMR. Interactions of protein side chains with RNA defined with REDOR solid state NMR. Interactions of protein side chains with RNA defined with REDOR solid state NMR. J Biomol NMR. 2011 Sep 25; Authors: Huang W, Varani G, Drobny GP Abstract Formation of the complex between human immunodeficiency virus type-1 Tat protein and the transactivation response region (TAR) RNA is vital for transcriptional elongation, yet the structure of the Tat-TAR complex remains to be established. The NMR structures of free TAR, and TAR bound to Tat-derived...	nmrlearner	Journal club	0	09-30-2011 05:59 AM
Interactions of protein side chains with RNA defined with REDOR solid state NMR Interactions of protein side chains with RNA defined with REDOR solid state NMR Abstract Formation of the complex between human immunodeficiency virus type-1 Tat protein and the transactivation response region (TAR) RNA is vital for transcriptional elongation, yet the structure of the Tat-TAR complex remains to be established. The NMR structures of free TAR, and TAR bound to Tat-derived peptides have been obtained by solution NMR, but only a small number of intermolecular NOEs could be identified unambiguously, preventing the determination of a complete structure. Here we show that a...	nmrlearner	Journal club	0	09-27-2011 07:04 AM
Measurement of multiple torsional angles from one-dimensional solid-state NMR spectra: application to the conformational analysis of a ligand in its biological receptor site. Measurement of multiple torsional angles from one-dimensional solid-state NMR spectra: application to the conformational analysis of a ligand in its biological receptor site. Measurement of multiple torsional angles from one-dimensional solid-state NMR spectra: application to the conformational analysis of a ligand in its biological receptor site. Phys Chem Chem Phys. 2010 Nov 14;12(42):13999-4008 Authors: Edwards R, Madine J, Fielding L, Middleton DA Knowledge of the three-dimensional structure of a ligand in the binding site of its biological...	nmrlearner	Journal club	0	02-04-2011 11:34 AM
[NMR paper] Monitoring tat peptide binding to TAR RNA by solid-state 31P-19F REDOR NMR. Monitoring tat peptide binding to TAR RNA by solid-state 31P-19F REDOR NMR. Related Articles Monitoring tat peptide binding to TAR RNA by solid-state 31P-19F REDOR NMR. Nucleic Acids Res. 2005;33(11):3447-54 Authors: Olsen GL, Edwards TE, Deka P, Varani G, Sigurdsson ST, Drobny GP Complexes of the HIV transactivation response element (TAR) RNA with the viral regulatory protein tat are of special interest due in particular to the plasticity of the RNA at this binding site and to the potential for therapeutic targeting of the interaction. We...	nmrlearner	Journal club	0	11-24-2010 11:14 PM
[NMR paper] Solid state NMR measurements of conformation and conformational distributions in the Solid state NMR measurements of conformation and conformational distributions in the membrane-bound HIV-1 fusion peptide. Related Articles Solid state NMR measurements of conformation and conformational distributions in the membrane-bound HIV-1 fusion peptide. J Mol Graph Model. 2001;19(1):129-35 Authors: Yang J, Parkanzky PD, Khunte BA, Canlas CG, Yang R, Gabrys CM, Weliky DP The solid state NMR lineshape of a protein backbone carbonyl nucleus is a general diagnostic of the local conformational distribution in the vicinity of that nucleus. In...	nmrlearner	Journal club	0	11-19-2010 08:32 PM
[NMR paper] Practical methods for solid-state NMR distance measurements on large biomolecules: co Practical methods for solid-state NMR distance measurements on large biomolecules: constant-time rotational resonance. Related Articles Practical methods for solid-state NMR distance measurements on large biomolecules: constant-time rotational resonance. J Magn Reson. 1999 Aug;139(2):371-6 Authors: Balazs YS, Thompson LK Simple modifications of the rotational resonance experiment substantially reduce the total experimental time needed to measure weak homonuclear dipolar couplings, a critical factor for achieving routine internuclear distance...	nmrlearner	Journal club	0	11-18-2010 08:31 PM
[NMR paper] Solid-state REDOR NMR distance measurements at the ligand site of a bacterial chemota Solid-state REDOR NMR distance measurements at the ligand site of a bacterial chemotaxis membrane receptor. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-acspubs.jpg Related Articles Solid-state REDOR NMR distance measurements at the ligand site of a bacterial chemotaxis membrane receptor. Biochemistry. 1997 Feb 18;36(7):1699-703 Authors: Wang J, Balazs YS, Thompson LK The Escherichia coli serine receptor senses serine levels in the environment and transmits this information across the bacterial inner membrane to...	nmrlearner	Journal club	0	08-22-2010 03:03 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off