In the last decades, ligand binding to human TSPO has been largely used in clinical neuroimaging, but little is known about the interaction mechanism. Protein conformational mobility plays a key role in the ligand recognition and both, ligand-free and ligand-bound structures, are mandatory for characterizing the molecular binding mechanism. In the absence of crystals for mammalian TSPO, we have exploited solid-state nuclear magnetic resonance (ssNMR) spectroscopy under magic-angle spinning (MAS)...
[NMR paper] Structure and Dynamics of the Rhomboid Protease GlpG in Liposomes Studied by Solid-State NMR.
Structure and Dynamics of the Rhomboid Protease GlpG in Liposomes Studied by Solid-State NMR.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles Structure and Dynamics of the Rhomboid Protease GlpG in Liposomes Studied by Solid-State NMR.
J Am Chem Soc. 2019 10 30;141(43):17314-17321
Authors: Shi C, Öster C, Bohg C, Li L, Lange S, Chevelkov V, Lange A
Abstract
Rhomboid proteases are intramembrane proteases that hydrolyze substrate peptide bonds within...
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[NMR paper] Solid-State NMR of Membrane Protein Reconstituted in Proteoliposomes, the Case of TSPO.
Solid-State NMR of Membrane Protein Reconstituted in Proteoliposomes, the Case of TSPO.
Related Articles Solid-State NMR of Membrane Protein Reconstituted in Proteoliposomes, the Case of TSPO.
Methods Mol Biol. 2017;1635:329-344
Authors: Senicourt L, Duma L, Papadopoulos V, Lacapere JJ
Abstract
Structural studies of membrane proteins (MP) in a native or native-like environment remain a challenge. X-ray crystallography of three-dimensional crystals of MP in lipids and cryo-electron microscopy of two-dimensional crystals also...
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07-30-2017 08:04 PM
Structural heterogeneity in microcrystalline ubiquitin studied by solid-state NMR
Structural heterogeneity in microcrystalline ubiquitin studied by solid-state NMR
Abstract
By applying - and -glucose labeling schemes to the folded globular protein ubiquitin, a strong reduction of spectral crowding and increase in resolution in solid-state NMR (ssNMR) spectra could be achieved. This allowed spectral resonance assignment in a straightforward manner and the collection of a wealth of long-range distance information. A high precision solid-state NMR structure of microcrystalline ubiquitin was calculated with a backbone rmsd of 1.57 to the X-ray structure and 1.32 Å to the...
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[NMR paper] Structural heterogeneity in microcrystalline ubiquitin studied by solid-state NMR.
Structural heterogeneity in microcrystalline ubiquitin studied by solid-state NMR.
Related Articles Structural heterogeneity in microcrystalline ubiquitin studied by solid-state NMR.
Protein Sci. 2015 Feb 2;
Authors: Fasshuber HK, Lakomek NA, Habenstein B, Loquet A, Shi C, Giller K, Wolff S, Becker S, Lange A
Abstract
By applying - and -glucose labeling schemes to the folded globular protein ubiquitin, a strong reduction of spectral crowding and increase in resolution in solid-state NMR (ssNMR) spectra could be achieved....
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02-04-2015 09:57 PM
Structural heterogeneity in microcrystalline ubiquitin studied by solid-state NMR
Structural heterogeneity in microcrystalline ubiquitin studied by solid-state NMR
ABSTRACT
By applying - and -glucose labeling schemes to the folded globular protein ubiquitin, a strong reduction of spectral crowding and increase in resolution in solid-state NMR (ssNMR) spectra could be achieved. This allowed spectral resonance assignment in a straightforward manner and the collection of a wealth of long-range distance information. A high precision solid-state NMR structure of microcrystalline ubiquitin was calculated with a backbone rmsd of 1.57 to the X-ray structure and 1.32 Å to the...
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02-02-2015 09:55 PM
Detergent Optimized Membrane Protein Reconstitutionin Liposomes for Solid State NMR
Detergent Optimized Membrane Protein Reconstitutionin Liposomes for Solid State NMR
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/bi500144h/aop/images/medium/bi-2014-00144h_0008.gif
Biochemistry
DOI: 10.1021/bi500144h
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[NMR paper] Detergent Optimized Membrane Protein Reconstitution in Liposomes for Solid State NMR.
Detergent Optimized Membrane Protein Reconstitution in Liposomes for Solid State NMR.
Related Articles Detergent Optimized Membrane Protein Reconstitution in Liposomes for Solid State NMR.
Biochemistry. 2014 Mar 25;
Authors: Murray DT, Griffin JM, Cross TA
Abstract
For small helical membrane proteins their structure is highly sensitive to their environment and solid state NMR is a structural technique that can characterize these membrane proteins in native like lipid bilayers and proteoliposomes. To date, a systematic method by which to...
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03-29-2014 01:00 PM
Investigation of the Interface in Silica-Encapsulated Liposomes by Combining Solid State NMR and First Principles Calculations
Investigation of the Interface in Silica-Encapsulated Liposomes by Combining Solid State NMR and First Principles Calculations
Nicolas Folliet, Claire Roiland, Sylvie Be?gu, Anne Aubert, Tzonka Mineva, Annick Goursot, Kaliaperumal Selvaraj, Luminita Duma, Frederik Tielens, Francesco Mauri, Guillaume Laurent, Christian Bonhomme, Christel Gervais, Florence Babonneau and Thierry Azai?s
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja201002r/aop/images/medium/ja-2011-01002r_0009.gif
Journal of the American Chemical Society
DOI:...
Solid-state NMR study of structural heterogeneity of the apo WT mouse TSPO reconstituted in liposomes
Linear Mode
