NMR paper Solid-state NMR studies of the mechanism of the opsin shift in the visual pigment rho

		BioNMR > Educational resources > Journal club
[NMR paper] Solid-state NMR studies of the mechanism of the opsin shift in the visual pigment rho

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

08-21-2010, 11:04 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,732

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Solid-state NMR studies of the mechanism of the opsin shift in the visual pigment rho

Solid-state NMR studies of the mechanism of the opsin shift in the visual pigment rhodopsin.

Related Articles Solid-state NMR studies of the mechanism of the opsin shift in the visual pigment rhodopsin.

Biochemistry. 1990 Sep 4;29(35):8158-64

Authors: Smith SO, Palings I, Miley ME, Courtin J, de Groot H, Lugtenburg J, Mathies RA, Griffin RG

Solid-state 13C NMR spectra have been obtained of bovine rhodopsin and isorhodopsin regenerated with retinal selectively 13C labeled along the polyene chain. In rhodopsin, the chemical shifts for 13C-5, 13C-6, 13C-7, 13C-14, and 13C-15 correspond closely to the chemical shifts observed in the 11-cis protonated Schiff base (PSB) model compound. Differences in chemical shift relative to the 11-cis PSB chloride salt are observed for positions 8 through 13, with the largest deshielding (6.2 ppm) localized at position 13. The localized deshielding at C-13 supports previous models of the opsin shift in rhodopsin that place a protein perturbation in the vicinity of position 13. Spectra obtained of isorhodopsin regenerated with 13C-labeled 9-cis-retinals reveal large perturbations at 13C-7 and 13C-13. The similar deshielding of the 13C-13 resonance in both pigments supports the presence of a protein perturbation near position 13. However, the chemical shifts at positions 7 and 12 in isorhodopsin are not analogous to those observed in rhodopsin and suggest that the binding site interactions near these positions are different for the two pigments. The implications of these results for the mechanism of the opsin shift in these proteins are discussed.

PMID: 2261469 [PubMed - indexed for MEDLINE]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] Magic-angle spinning solid-state NMR spectroscopy of the beta1 immunoglobulin binding domain of protein G (GB1): 15N and 13C chemical shift assignments and conformational analysis. Magic-angle spinning solid-state NMR spectroscopy of the beta1 immunoglobulin binding domain of protein G (GB1): 15N and 13C chemical shift assignments and conformational analysis. Related Articles Magic-angle spinning solid-state NMR spectroscopy of the beta1 immunoglobulin binding domain of protein G (GB1): 15N and 13C chemical shift assignments and conformational analysis. J Am Chem Soc. 2005 Sep 7;127(35):12291-305 Authors: Franks WT, Zhou DH, Wylie BJ, Money BG, Graesser DT, Frericks HL, Sahota G, Rienstra CM Magic-angle spinning...	nmrlearner	Journal club	0	12-01-2010 06:56 PM
[NMR paper] Structure of an elastin-mimetic polypeptide by solid-state NMR chemical shift analysi Structure of an elastin-mimetic polypeptide by solid-state NMR chemical shift analysis. Related Articles Structure of an elastin-mimetic polypeptide by solid-state NMR chemical shift analysis. Biopolymers. 2003 Oct;70(2):158-68 Authors: Hong M, Isailovic D, McMillan RA, Conticello VP The conformation of an elastin-mimetic recombinant protein, 39, is investigated using solid-state NMR spectroscopy. The protein is extensively labeled with 13C and 15N, and two-dimensional 13C-13C and 15N-13C correlation experiments were carried out to resolve and...	nmrlearner	Journal club	0	11-24-2010 09:16 PM
[NMR paper] Solid-state NMR studies of the membrane-bound closed state of the colicin E1 channel Solid-state NMR studies of the membrane-bound closed state of the colicin E1 channel domain in lipid bilayers. Related Articles Solid-state NMR studies of the membrane-bound closed state of the colicin E1 channel domain in lipid bilayers. Protein Sci. 1998 Feb;7(2):342-8 Authors: Kim Y, Valentine K, Opella SJ, Schendel SL, Cramer WA The colicin E1 channel polypeptide was shown to be organized anisotropically in membranes by solid-state NMR analysis of samples of uniformly 15N-labeled protein in oriented planar phospholipid bilayers. The 190...	nmrlearner	Journal club	0	11-17-2010 11:06 PM
New NMR Book: Solid-State NMR Studies of Biopolymers New NMR Book: Solid-State NMR Studies of Biopolymers edited by Anne McDermott, Tatyana Polenova Hardcover: 592 pages Publisher: Wiley; September 2010 Language: English ISBN: 978-0470721223 http://www.amazon.com/dp/0470721227 http://www.amazon.ca/dp/0470721227	nmrlearner	Books	0	10-05-2010 02:04 AM
[NMR paper] NMR studies of fluorinated visual pigment analogs. NMR studies of fluorinated visual pigment analogs. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles NMR studies of fluorinated visual pigment analogs. Biochem Biophys Res Commun. 1991 Sep 30;179(3):1337-43 Authors: Colmenares LU, Asato AE, Denny M, Mead D, Zingoni JP, Liu RS The 19F-nmr chemical shift data of isomeric pigments (11-cis and 9-cis) of four vinyl fluororhodopsins and two trifluororhodopsins have been recorded. When compared with model protonated Schiff...	nmrlearner	Journal club	0	08-21-2010 11:12 PM
[NMR paper] NMR studies of fluorinated visual pigment analogs. NMR studies of fluorinated visual pigment analogs. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles NMR studies of fluorinated visual pigment analogs. Biochem Biophys Res Commun. 1991 Sep 30;179(3):1337-43 Authors: Colmenares LU, Asato AE, Denny M, Mead D, Zingoni JP, Liu RS The 19F-nmr chemical shift data of isomeric pigments (11-cis and 9-cis) of four vinyl fluororhodopsins and two trifluororhodopsins have been recorded. When compared with model protonated Schiff...	nmrlearner	Journal club	0	08-21-2010 11:12 PM
[NMR paper] Solid state 15N NMR evidence for a complex Schiff base counterion in the visual G-pro Solid state 15N NMR evidence for a complex Schiff base counterion in the visual G-protein-coupled receptor rhodopsin. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-acspubs.jpg Related Articles Solid state 15N NMR evidence for a complex Schiff base counterion in the visual G-protein-coupled receptor rhodopsin. Biochemistry. 1999 Jun 1;38(22):7195-9 Authors: Creemers AF, Klaassen CH, Bovee-Geurts PH, Kelle R, Kragl U, Raap J, de Grip WJ, Lugtenburg J, de Groot HJ Using the baculovirus/Sf9 cell expression system, we...	nmrlearner	Journal club	0	08-21-2010 04:03 PM
Hi guys I need to help to restate this small pragraph? Chemical shift?solid state physics? The chemical shift The chemical shift is one of the most important observables in nuclear magnetic resonance. It provides valuable information about the chemical environment around a nucleus. In a real spin system, nuclei are surrounded by atomic and molecular electron clouds which interact with the nuclear spin angular moment. The principal influence of the surrounding electrons is the magnetic screening which results when electronic orbitals are perturbed by the applied magnetic field BO. The effect of the magnetic screening (shielding), called nuclear shielding, can enhance or oppose the...	ISPolPH8789	NMR Questions and Answers	1	01-23-2005 03:06 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off