Related ArticlesSolid State NMR Studies of Intact Lipopolysaccharide Endotoxin.
ACS Chem Biol. 2018 Jul 02;:
Authors: Laguri C, Silipo A, Martorana AM, Schanda P, Marchetti R, Polissi A, Molinaro A, Simorre JP
Abstract
Lipopolysaccharides (LPS) are complex glycolipids forming the outside layer of Gram-negative bacteria. Their hydrophobic and heterogeneous nature greatly hampers their structural study in an environment similar to the bacterial surface. We have studied LPS purified from E. coli and pathogenic P. aeruginosa with long O-antigen polysaccharides assembled in solution as vesicles or elongated micelles. Solid-state NMR with magic-angle spinning permitted the identification of NMR signals arising from regions with different flexibilities in the LPS, from the lipid components to the O-antigen polysaccharides. Atomic scale data on the LPS enabled the study of the interaction of gentamicin antibiotic bound to P. aeruginosa LPS, for which we could confirm that a specific oligosaccharide is involved in the antibiotic binding. The possibility to study LPS alone and bound to a ligand when it is assembled in membrane-like structures opens great prospects for the investigation of proteins and antibiotics that specifically target such an important molecule at the surface of Gram-negative bacteria.
PMID: 29965728 [PubMed - as supplied by publisher]
[NMR paper] Spectral Comparisons of Mammalian Cells and Intact Organelles by Solid-State NMR.
Spectral Comparisons of Mammalian Cells and Intact Organelles by Solid-State NMR.
Related Articles Spectral Comparisons of Mammalian Cells and Intact Organelles by Solid-State NMR.
J Struct Biol. 2018 May 30;:
Authors: Werby SH, Cegelski L
Abstract
Whole-cell protein profiling, spatial localization, and quantification of activities such as gene transcription and protein translation are possible with modern biochemical and biophysical techniques. Yet, addressing questions of overall compositional changes within a cell - capturing...
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[NMR paper] Interaction of the antimicrobial peptides caerin 1.1 and aurein 1.2 with intact bacteria by (2)H solid-state NMR.
Interaction of the antimicrobial peptides caerin 1.1 and aurein 1.2 with intact bacteria by (2)H solid-state NMR.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Interaction of the antimicrobial peptides caerin 1.1 and aurein 1.2 with intact bacteria by (2)H solid-state NMR.
Biochim Biophys Acta. 2016 12;1858(12):2959-2964
Authors: Laadhari M, Arnold AA, Gravel AE, Separovic F, Marcotte I
Abstract
Nuclear magnetic resonance (NMR) is...
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[NMR paper] Recent progress on the application of (2)H solid-state NMR to probe the interaction of antimicrobial peptides with intact bacteria.
Recent progress on the application of (2)H solid-state NMR to probe the interaction of antimicrobial peptides with intact bacteria.
Related Articles Recent progress on the application of (2)H solid-state NMR to probe the interaction of antimicrobial peptides with intact bacteria.
Biochim Biophys Acta. 2017 Aug 24;:
Authors: Booth V, Warschawski DE, Santisteban NP, Laadhari M, Marcotte I
Abstract
Discoveries relating to innate immunity and antimicrobial peptides (AMPs) granted Bruce Beutler and Jules Hoffmann a Nobel prize in...
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[NMR paper] Ultra fast magic angle spinning solid - state NMR spectroscopy of intact bone.
Ultra fast magic angle spinning solid - state NMR spectroscopy of intact bone.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-7315-19-Wiley_FullText_120x30_orange.png Related Articles Ultra fast magic angle spinning solid - state NMR spectroscopy of intact bone.
Magn Reson Chem. 2016 Feb;54(2):132-5
Authors: Singh C, Rai RK, Kayastha AM, Sinha N
Abstract
Ultra fast magic angle spinning (MAS) has been a potent method to significantly average out homogeneous/inhomogeneous line...
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01-28-2017 08:29 PM
[NMR paper] Insight into the Supramolecular Architecture of Intact Diatom Biosilica from DNP-Supported Solid-State NMR Spectroscopy.
Insight into the Supramolecular Architecture of Intact Diatom Biosilica from DNP-Supported Solid-State NMR Spectroscopy.
Related Articles Insight into the Supramolecular Architecture of Intact Diatom Biosilica from DNP-Supported Solid-State NMR Spectroscopy.
Angew Chem Int Ed Engl. 2015 Oct 28;
Authors: Jantschke A, Koers E, Mance D, Weingarth M, Brunner E, Baldus M
Abstract
Diatom biosilica is an inorganic/organic hybrid with interesting properties. The molecular architecture of the organic material at the atomic and nanometer...
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10-29-2015 10:08 PM
Nucleotide-type chemical shift assignment of the encapsulated 40 kbp dsDNA in intact bacteriophage T7 by MAS solid-state NMR
Nucleotide-type chemical shift assignment of the encapsulated 40 kbp dsDNA in intact bacteriophage T7 by MAS solid-state NMR
Abstract
The icosahedral bacteriophage T7 is a 50 MDa double-stranded DNA (dsDNA) virus that infects Escherichia coli. Although there is substantial information on the physical and morphological properties of T7, structural information, based mostly on Raman spectroscopy and cryo-electron microscopy, is limited. Here, we apply the magic-angle spinning (MAS) solid-state NMR (SSNMR) technique to study a uniformly 13C and 15N...
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[NMR paper] Interactions of lipopolysaccharide with lipid membranes, raft models - a solid state NMR study.
Interactions of lipopolysaccharide with lipid membranes, raft models - a solid state NMR study.
Related Articles Interactions of lipopolysaccharide with lipid membranes, raft models - a solid state NMR study.
Biochim Biophys Acta. 2013 Apr 5;
Authors: Ciesielski F, Griffin DC, Rittig M, Moriyón I, Bonev BB
Abstract
Lipopolysaccharide (LPS) is a major component of the external leaflet of bacterial outer membranes, key pro-inflammatory factor and an important mediator of host-pathogen interactions. In host cells it activates the complement...
Solid State NMR Studies of Intact Lipopolysaccharide Endotoxin.
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