NMR paper Solid-state NMR spectroscopy applied to membrane proteins.

		BioNMR > Educational resources > Journal club
[NMR paper] Solid-state NMR spectroscopy applied to membrane proteins.

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

11-19-2010, 08:29 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Solid-state NMR spectroscopy applied to membrane proteins.

Solid-state NMR spectroscopy applied to membrane proteins.

Related Articles Solid-state NMR spectroscopy applied to membrane proteins.

Curr Opin Struct Biol. 2000 Oct;10(5):593-600

Authors: de Groot HJ

One major remaining problem in structural biology is to elucidate the structure and mechanism of function of membrane proteins. On the basis of preliminary information from genome projects, it is now estimated that up to 50,000 different membrane proteins may exist in the human being and that virtually every life process proceeds, sooner or later, through a membrane protein. Solid-state NMR spectroscopy in high magnetic field is rapidly developing into a widely applicable tool to describe the structure and help understand the mechanism of function of a membrane protein. Recent work in applied solid-state NMR spectroscopy crosses the boundary between the biological and the physical sciences, and aims at increasing the predictive range of this biophysical method.

PMID: 11042459 [PubMed - indexed for MEDLINE]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Fractional deuteration applied to biomolecular solid-state NMR spectroscopy Fractional deuteration applied to biomolecular solid-state NMR spectroscopy Abstract Solid-state Nuclear Magnetic Resonance can provide detailed insight into structural and dynamical aspects of complex biomolecules. With increasing molecular size, advanced approaches for spectral simplification and the detection of medium to long-range contacts become of critical relevance. We have analyzed the protonation pattern of a membrane-embedded ion channel that was obtained from bacterial expression using protonated precursors and D2O medium. We find an overall reduction of 50% in protein...	nmrlearner	Journal club	0	11-24-2011 12:25 AM
Proton-Detected Solid-State NMR Spectroscopy of Fibrillar and Membrane Proteins. Proton-Detected Solid-State NMR Spectroscopy of Fibrillar and Membrane Proteins. Proton-Detected Solid-State NMR Spectroscopy of Fibrillar and Membrane Proteins. Angew Chem Int Ed Engl. 2011 Apr 20; Authors: Linser R, Dasari M, Hiller M, Higman V, Fink U, Lopez Del Amo JM, Markovic S, Handel L, Kessler B, Schmieder P, Oesterhelt D, Oschkinat H, Reif B	nmrlearner	Journal club	0	04-22-2011 02:00 PM
Proton-Detected Solid-State NMR Spectroscopy of Fibrillar and Membrane Proteins. Proton-Detected Solid-State NMR Spectroscopy of Fibrillar and Membrane Proteins. Proton-Detected Solid-State NMR Spectroscopy of Fibrillar and Membrane Proteins. Angew Chem Int Ed Engl. 2011 Apr 14; Authors: Linser R, Dasari M, Hiller M, Higman V, Fink U, Lopez Del Amo JM, Markovic S, Handel L, Kessler B, Schmieder P, Oesterhelt D, Oschkinat H, Reif B	nmrlearner	Journal club	0	04-16-2011 12:29 PM
Structure and dynamics of cationic membrane peptides and proteins: Insights from solid-state NMR. Structure and dynamics of cationic membrane peptides and proteins: Insights from solid-state NMR. Structure and dynamics of cationic membrane peptides and proteins: Insights from solid-state NMR. Protein Sci. 2011 Feb 22; Authors: Hong M, Su Y Many membrane peptides and protein domains contain functionally important cationic Arg and Lys residues, whose insertion into the hydrophobic interior of the lipid bilayer encounters significant energy barriers. To understand how these cationic molecules overcome the free energy barrier to insert into the...	nmrlearner	Journal club	0	02-24-2011 11:04 AM
[NMR paper] How to prepare membrane proteins for solid-state NMR: A case study on the alpha-helical integral membrane protein diacylglycerol kinase from E. coli. How to prepare membrane proteins for solid-state NMR: A case study on the alpha-helical integral membrane protein diacylglycerol kinase from E. coli. Related Articles How to prepare membrane proteins for solid-state NMR: A case study on the alpha-helical integral membrane protein diacylglycerol kinase from E. coli. Chembiochem. 2005 Sep;6(9):1693-700 Authors: Lorch M, Faham S, Kaiser C, Weber I, Mason AJ, Bowie JU, Glaubitz C Several studies have demonstrated that it is viable to use microcrystalline preparations of water-soluble proteins as...	nmrlearner	Journal club	0	12-01-2010 06:56 PM
[NMR paper] Site-directed 13C solid-state NMR studies on membrane proteins: strategy and goals to Site-directed 13C solid-state NMR studies on membrane proteins: strategy and goals toward revealing conformation and dynamics as illustrated for bacteriorhodopsin labeled with amino acid residues. Related Articles Site-directed 13C solid-state NMR studies on membrane proteins: strategy and goals toward revealing conformation and dynamics as illustrated for bacteriorhodopsin labeled with amino acid residues. Magn Reson Chem. 2004 Feb;42(2):218-30 Authors: Saitô H, Mikami J, Yamaguchi S, Tanio M, Kira A, Arakawa T, Yamamoto K, Tuzi S We have so...	nmrlearner	Journal club	0	11-24-2010 09:25 PM
[NMR paper] Membrane structure and dynamics as viewed by solid-state NMR spectroscopy. Membrane structure and dynamics as viewed by solid-state NMR spectroscopy. Related Articles Membrane structure and dynamics as viewed by solid-state NMR spectroscopy. Biophys Chem. 1997 Oct;68(1-3):233-41 Authors: Auger M The purpose of the present study is the investigation of the structure and dynamics of biological membranes using solid-state nuclear magnetic resonance (NMR) spectroscopy. Two approaches are used in our laboratory. The first involves the measurement of high-resolution 13C and 1H spectra obtained by the magic angle spinning...	nmrlearner	Journal club	0	08-22-2010 05:08 PM
Solid State NMR of membrane peptides and proteins Solid State NMR of membrane peptides and proteins Lecture notes on "Solid State NMR of membrane peptides and proteins" by Dr. SK Straus from Univ. of British Columbia More...	nmrlearner	General	0	08-16-2010 03:50 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off