Solid state NMR spectral editing of histidine, arginine and lysine using Hadamard encoding
 

Solid state NMR spectral editing of histidine, arginine and lysine using Hadamard encoding

The NMR signals from protein sidechains are rich in information about intra- and inter-molecular interactions, but their detection can be complicated due to spectral overlap as well as conformational and hydrogen exchange. In this work, we demonstrate a protocol for multi-dimensional solid-state NMR spectral editing of signals from basic sidechains based on Hadamard matrix encoding. The Hadamard method acquires multi-dimensional experiments in such a way that both the backbone and under-sampled...

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