Solid-state NMR of proteins sedimented by ultracentrifugation.

		BioNMR > Educational resources > Journal club
Solid-state NMR of proteins sedimented by ultracentrifugation.

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

06-15-2011, 01:15 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Solid-state NMR of proteins sedimented by ultracentrifugation.

Solid-state NMR of proteins sedimented by ultracentrifugation.

Solid-state NMR of proteins sedimented by ultracentrifugation.

Proc Natl Acad Sci U S A. 2011 Jun 13;

Authors: Bertini I, Luchinat C, Parigi G, Ravera E, Reif B, Turano P

Relatively large proteins in solution, spun in NMR rotors for solid samples at typical ultracentrifugation speeds, sediment at the rotor wall. The sedimented proteins provide high-quality solid-state-like NMR spectra suitable for structural investigation. The proteins fully revert to the native solution state when spinning is stopped, allowing one to study them in both conditions. Transiently sedimented proteins can be considered a novel phase as far as NMR is concerned. NMR of transiently sedimented molecules under fast magic angle spinning has the advantage of overcoming protein size limitations of solution NMR without the need of sample crystallization/precipitation required by solid-state NMR.

PMID: 21670262 [PubMed - as supplied by publisher]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Solid-state NMR of proteins sedimented by ultracentrifugation [Chemistry] Solid-state NMR of proteins sedimented by ultracentrifugation Bertini, I., Luchinat, C., Parigi, G., Ravera, E., Reif, B., Turano, P.... Date: 2011-06-28 Relatively large proteins in solution, spun in NMR rotors for solid samples at typical ultracentrifugation speeds, sediment at the rotor wall. The sedimented proteins provide high-quality solid-state-like NMR spectra suitable for structural investigation. The proteins fully revert to the native solution state when spinning is stopped, allowing one to study them in both conditions. Transiently sedimented proteins can be considered a...	nmrlearner	Journal club	0	06-29-2011 04:45 AM
[NMR paper] Solid-state 17O NMR as a probe for structural studies of proteins in biomembranes. Solid-state 17O NMR as a probe for structural studies of proteins in biomembranes. Related Articles Solid-state 17O NMR as a probe for structural studies of proteins in biomembranes. J Am Chem Soc. 2004 Dec 1;126(47):15320-1 Authors: Lemaître V, de Planque MR, Howes AP, Smith ME, Dupree R, Watts A We report the first example of 17O NMR spectra from a selectively labeled transmembrane peptide, 17O--WALP23, as a lyophilized powder and incorporated in hydrated phospholipid vesicles. It is shown that at high magnetic field it is feasible to apply...	nmrlearner	Journal club	0	11-24-2010 10:03 PM
[NMR paper] Resolution enhancement in multidimensional solid-state NMR spectroscopy of proteins u Resolution enhancement in multidimensional solid-state NMR spectroscopy of proteins using spin-state selection. Related Articles Resolution enhancement in multidimensional solid-state NMR spectroscopy of proteins using spin-state selection. J Am Chem Soc. 2003 Oct 1;125(39):11816-7 Authors: Duma L, Hediger S, Brutscher B, Böckmann A, Emsley L We show that the resolution of homonuclear multidimensional solid-state NMR correlation experiments can be significantly improved using transition selection and spin-state-selective polarization transfer...	nmrlearner	Journal club	0	11-24-2010 09:16 PM
[NMR paper] Solid-state NMR studies of the structure and mechanisms of proteins. Solid-state NMR studies of the structure and mechanisms of proteins. Related Articles Solid-state NMR studies of the structure and mechanisms of proteins. Curr Opin Struct Biol. 2002 Oct;12(5):661-9 Authors: Thompson LK Magic-angle spinning solid-state NMR experiments are well suited to investigating the structures and mechanisms of important proteins that are inaccessible to X-ray crystallography and solution NMR spectroscopy, including membrane proteins and disease-related protein aggregates. Good progress has been made in the development...	nmrlearner	Journal club	0	11-24-2010 08:58 PM
[NMR paper] Solid-state NMR spectroscopy applied to membrane proteins. Solid-state NMR spectroscopy applied to membrane proteins. Related Articles Solid-state NMR spectroscopy applied to membrane proteins. Curr Opin Struct Biol. 2000 Oct;10(5):593-600 Authors: de Groot HJ One major remaining problem in structural biology is to elucidate the structure and mechanism of function of membrane proteins. On the basis of preliminary information from genome projects, it is now estimated that up to 50,000 different membrane proteins may exist in the human being and that virtually every life process proceeds, sooner or...	nmrlearner	Journal club	0	11-19-2010 08:29 PM
GFT projection NMR spectroscopy for proteins in the solid state. GFT projection NMR spectroscopy for proteins in the solid state. GFT projection NMR spectroscopy for proteins in the solid state. J Biomol NMR. 2010 Oct 30; Authors: Trent Franks W, Atreya HS, Szyperski T, Rienstra CM Recording of four-dimensional (4D) spectra for proteins in the solid state has opened new avenues to obtain virtually complete resonance assignments and three-dimensional (3D) structures of proteins. As in solution state NMR, the sampling of three indirect dimensions leads per se to long minimal measurement time. Furthermore,...	nmrlearner	Journal club	0	11-06-2010 11:02 AM
[NMR paper] Dynamic structure of proteins in solid state. 1H and 13C NMR relaxation study. Dynamic structure of proteins in solid state. 1H and 13C NMR relaxation study. Related Articles Dynamic structure of proteins in solid state. 1H and 13C NMR relaxation study. J Biomol Struct Dyn. 1996 Oct;14(2):211-24 Authors: Krushelnitsky AG, Fedotov VD, Spevacek J, Straka J Temperature dependencies of 1H non-selective NMR T1 and T2 relaxation times measured at two resonance frequencies and natural abundance 13C NMR relaxation times T1 and T1r measured at room temperature have been studied in a set of dry and wet solid proteins - Bacterial...	nmrlearner	Journal club	0	08-22-2010 02:20 PM
Solid State NMR of membrane peptides and proteins Solid State NMR of membrane peptides and proteins Lecture notes on "Solid State NMR of membrane peptides and proteins" by Dr. SK Straus from Univ. of British Columbia More...	nmrlearner	General	0	08-16-2010 03:50 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off