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Old 11-11-2014, 11:57 AM
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Default Solid-state NMR of a protein in a precipitated complex with a full-length antibody.

Solid-state NMR of a protein in a precipitated complex with a full-length antibody.

Solid-state NMR of a protein in a precipitated complex with a full-length antibody.

J Am Chem Soc. 2014 Nov 10;

Authors: Lamley JM, Iuga D, Oster C, Sass HJ, Rogowski M, Oss A, Past J, Reinhold A, Grzesiek S, Samoson A, Lewandowski JR

Abstract
NMR is a prime technique for characterizing atomic resolution structures and dynamics of biomolecular complexes but, for such systems, faces challenges of sensitivity and spectral resolution. We demonstrate that application of (1)H-detected experiments at >50 kHz magic angle spinning frequencies enables the recording, in a matter of minutes to hours, of solid-state NMR spectra suitable for quantitative analysis of protein complexes present in quantities as small as a few nanomoles (tens of micrograms for the observed component). This approach enables direct structure determination and quantitative dynamics measurements in domains of hundreds-of-kDa protein complexes. Protein-protein interaction interfaces can be mapped out by comparing the chemical shifts of proteins within solid-state complexes with those of the same constituent proteins free in solution. We employ this methodology to characterize a >300 kDa complex of GB1 with full-length human immunoglobulin, where we find that sample preparation by simple precipitation yields spectra of exceptional quality, a feature that is likely to be shared with some other precipitating complexes. Finally, we investigate extensions of our methodology to spinning frequencies of up to 100 kHz.


PMID: 25381931 [PubMed - as supplied by publisher]



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