NMR paper Solid-state NMR methods for oriented membrane proteins.

		BioNMR > Educational resources > Journal club
[NMR paper] Solid-state NMR methods for oriented membrane proteins.

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

08-19-2015, 03:24 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Solid-state NMR methods for oriented membrane proteins.

Solid-state NMR methods for oriented membrane proteins.

Related Articles Solid-state NMR methods for oriented membrane proteins.

Prog Nucl Magn Reson Spectrosc. 2015 Aug;88-89:48-85

Authors: Hansen SK, Bertelsen K, Paaske B, Nielsen NC, Vosegaard T

Abstract
Oriented-sample solid-state NMR represents one of few experimental methods capable of characterising the membrane-bound conformation of proteins in the cell membrane. Since the technique was developed 25 years ago, the technique has been applied to study the structure of helix bundle membrane proteins and antimicrobial peptides, characterise protein-lipid interactions, and derive information on dynamics of the membrane anchoring of membrane proteins. We will review the major developments in various aspects of oriented-sample solid-state NMR, including sample-preparation methods, pulse sequences, theory required to interpret the experiments, perspectives for and guidelines to new experiments, and a number of representative applications.

PMID: 26282196 [PubMed - in process]

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Solid-state NMR methods for oriented membrane proteins From The DNP-NMR Blog: Solid-state NMR methods for oriented membrane proteins This review article gives a comprehensive overview of the field of solid-state NMR spectroscopy on oriented samples. These experiments are typically less demanding on instrumentation since experiments are performed on static samples, without the need of spinning. While this article is not directly related to DNP-NMR spectroscopy, DNP-NMR on static samples will certainly become more interesting in the future.	nmrlearner	News from NMR blogs	0	08-04-2015 01:43 AM
Solid-State NMR Methods for Oriented Membrane Proteins Solid-State NMR Methods for Oriented Membrane Proteins Publication date: Available online 19 May 2015 Source:Progress in Nuclear Magnetic Resonance Spectroscopy</br> Author(s): Sara K. Hansen , Kresten Bertelsen , Berit Paaske , Niels Chr. Nielsen , Thomas Vosegaard</br> Oriented-sample solid-state NMR represents one of few experimental methods capable of characterising the membrane-bound conformation of proteins in the cell membrane. Since the technique was developed 25 years ago, the technique has been applied to study the structure of helix bundle membrane...	nmrlearner	Journal club	0	05-19-2015 09:10 AM
Dynamic and Contrasting Information by Oriented-Sample Solid-State NMR Spectroscopy of Membrane Proteins Dynamic and Contrasting Information by Oriented-Sample Solid-State NMR Spectroscopy of Membrane Proteins Publication date: 28 January 2014 Source:Biophysical Journal, Volume 106, Issue 2, Supplement 1</br> Author(s): Alexander Nevzorov , Deanna M. Tesch</br> </br></br> </br></br> More...	nmrlearner	Journal club	0	01-29-2014 12:50 AM
[NMR paper] Sensitivity and resolution enhancement of oriented solid-state NMR: Application to membrane proteins. Sensitivity and resolution enhancement of oriented solid-state NMR: Application to membrane proteins. Related Articles Sensitivity and resolution enhancement of oriented solid-state NMR: Application to membrane proteins. Prog Nucl Magn Reson Spectrosc. 2013 Nov;75:50-68 Authors: Gopinath T, Mote KR, Veglia G Abstract Oriented solid-state NMR (O-ssNMR) spectroscopy is a major technique for the high-resolution analysis of the structure and topology of transmembrane proteins in native-like environments. Unlike magic angle spinning (MAS)...	nmrlearner	Journal club	0	10-29-2013 08:21 PM
[NMR paper] Lipid bilayer preparations of membrane proteins for oriented and magic-angle spinning solid-state NMR samples. Lipid bilayer preparations of membrane proteins for oriented and magic-angle spinning solid-state NMR samples. Related Articles Lipid bilayer preparations of membrane proteins for oriented and magic-angle spinning solid-state NMR samples. Nat Protoc. 2013 Nov;8(11):2256-70 Authors: Das N, Murray DT, Cross TA Abstract	nmrlearner	Journal club	0	10-27-2013 12:53 AM
Sensitivity and Resolution Enhancement of Oriented Solid-State NMR: Application to Membrane Proteins Sensitivity and Resolution Enhancement of Oriented Solid-State NMR: Application to Membrane Proteins Publication date: Available online 12 August 2013 Source:Progress in Nuclear Magnetic Resonance Spectroscopy</br> Author(s): T. Gopinath , Kaustubh R. Mote , Gianluigi Veglia</br> Oriented solid-state NMR (O-ssNMR) spectroscopy is a major technique for the high-resolution analysis of the structure and topology of transmembrane proteins in native-like environments. Unlike magic angle spinning (MAS) techniques, O-ssNMR spectroscopy requires membrane protein...	nmrlearner	Journal club	0	08-13-2013 04:09 AM
Improved 1H amide resonance line narrowing in oriented sample solid-state NMR of membrane proteins in phospholipid bilayers Improved 1H amide resonance line narrowing in oriented sample solid-state NMR of membrane proteins in phospholipid bilayers July 2012 Publication year: 2012 Source:Journal of Magnetic Resonance, Volume 220</br> </br> We demonstrate 1H amide resonance line widths	nmrlearner	Journal club	0	02-03-2013 10:13 AM
Improved 1H Amide Resonance Line Narrowing in Oriented Sample Solid-state NMR of Membrane Proteins in Phospholipid Bilayers Improved 1H Amide Resonance Line Narrowing in Oriented Sample Solid-state NMR of Membrane Proteins in Phospholipid Bilayers Publication year: 2012 Source:Journal of Magnetic Resonance</br> George J. Lu, Sang Ho Park, Stanley J. Opella</br> We demonstrate 1H amide resonance line widths	nmrlearner	Journal club	0	04-26-2012 08:10 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off