Protein solid-state NMR has evolved dramatically over the last two decades, with the development of new hardware and sample preparation methodologies. This technique is now ripe for complex applications, among which one can count bioconjugation, protein chemistry and functional biomaterials. In this review, we provide our account on this aspect of protein solid-state NMR.
[NMR paper] Static solid-state (2)H NMR methods in studies of protein side-chain dynamics.
Static solid-state (2)H NMR methods in studies of protein side-chain dynamics.
Related Articles Static solid-state (2)H NMR methods in studies of protein side-chain dynamics.
Prog Nucl Magn Reson Spectrosc. 2017 Aug;101:1-17
Authors: Vugmeyster L, Ostrovsky D
Abstract
In this review, we discuss the experimental static deuteron NMR techniques and computational approaches most useful for the investigation of side-chain dynamics in protein systems. Focus is placed on the interpretation of line shape and relaxation data within the...
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08-29-2017 05:35 PM
[NMR paper] Proton-Detected NMR Spectroscopy of Nanodisc-Embedded Membrane Proteins: MAS Solid-State vs. Solution-State Methods.
Proton-Detected NMR Spectroscopy of Nanodisc-Embedded Membrane Proteins: MAS Solid-State vs. Solution-State Methods.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles Proton-Detected NMR Spectroscopy of Nanodisc-Embedded Membrane Proteins: MAS Solid-State vs. Solution-State Methods.
J Phys Chem B. 2017 Jul 24;:
Authors: Lakomek NA, Frey L, Bibow S, Böckmann A, Riek R, Meier BH
Abstract
The structural and dynamical characterization of membrane proteins...
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07-25-2017 07:46 PM
Static Solid-state 2H NMR Methods in Studies of Protein Side-chain Dynamics
Static Solid-state 2H NMR Methods in Studies of Protein Side-chain Dynamics
Publication date: Available online 14 March 2017
Source:Progress in Nuclear Magnetic Resonance Spectroscopy</br>
Author(s): Liliya Vugmeyster, Dmitry Ostrovsky</br>
In this review, we discuss the experimental static deuteron NMR techniques and computational approaches most useful for the investigation of side-chain dynamics in protein systems. Focus is placed on the interpretation of line shape and relaxation data within the framework of motional modeling. We consider both jump and...
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03-14-2017 08:16 AM
[NMR paper] Spin-state-selective methods in solution- and solid-state biomolecular (13)C NMR.
Spin-state-selective methods in solution- and solid-state biomolecular (13)C NMR.
Related Articles Spin-state-selective methods in solution- and solid-state biomolecular (13)C NMR.
Prog Nucl Magn Reson Spectrosc. 2015 Feb;84-85C:1-13
Authors: Felli IC, Pierattelli R
Abstract
Spin-state-selective methods to achieve homonuclear decoupling in the direct acquisition dimension of (13)C detected NMR experiments have been one of the key contributors to converting (13)C detected NMR experiments into really useful tools for studying...
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02-12-2015 07:48 PM
Spin-State-Selective Methods in Solution- and Solid-State Biomolecular 13C NMR
Spin-State-Selective Methods in Solution- and Solid-State Biomolecular 13C NMR
Publication date: Available online 1 November 2014
Source:Progress in Nuclear Magnetic Resonance Spectroscopy</br>
Author(s): Isabella C. Felli , Roberta Pierattelli</br>
Spin-state-selective methods to achieve homonuclear decoupling in the direct acquisition dimension of 13C detected NMR experiments have been one of the key contributors to converting 13C detected NMR experiments into really useful tools for studying biomolecules. We discuss here in detail the various methods that have...
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11-02-2014 03:51 PM
[NMR paper] NMR and computational methods in the structural and dynamic characterization of ligand-receptor interactions.
NMR and computational methods in the structural and dynamic characterization of ligand-receptor interactions.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--production.springer.de-OnlineResources-Logos-springerlink.gif Related Articles NMR and computational methods in the structural and dynamic characterization of ligand-receptor interactions.
Adv Exp Med Biol. 2014;805:271-304
Authors: Ghitti M, Musco G, Spitaleri A
Abstract
The recurrent failures in drug discovery campaigns, the asymmetry between the...
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[NMR paper] Characterization of protein-ligand interactions by high-resolution solid-state NMR sp
Characterization of protein-ligand interactions by high-resolution solid-state NMR spectroscopy.
Related Articles Characterization of protein-ligand interactions by high-resolution solid-state NMR spectroscopy.
J Am Chem Soc. 2004 Nov 3;126(43):13948-53
Authors: Zech SG, Olejniczak E, Hajduk P, Mack J, McDermott AE
A novel approach for detection of ligand binding to a protein in solid samples is described. Hydrated precipitates of the anti-apoptotic protein Bcl-xL show well-resolved (13)C-(13)C 2D solid-state NMR spectra that allow...
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11-24-2010 10:03 PM
[NMR paper] Characterization of tertiary interactions in a folded protein by NMR methods: studies
Characterization of tertiary interactions in a folded protein by NMR methods: studies of pH-induced structural changes in human growth hormone.
Related Articles Characterization of tertiary interactions in a folded protein by NMR methods: studies of pH-induced structural changes in human growth hormone.
Biochemistry. 1992 Sep 15;31(36):8587-96
Authors: Abildgaard F, Jørgensen AM, Led JJ, Christensen T, Jensen EB, Junker F, Dalbøge H
The pH-induced conformational changes in human growth hormone (hGH) have been studied, using a new...
Solid-state NMR methods for the characterization of bioconjugations and protein-material interactions
Linear Mode
