Membranes of cells are active barriers, in which membrane proteins perform essential remodelling, transport and recognition functions that are vital to cells. Membrane proteins are key regulatory components of cells and represent essential targets for the modulation of cell function and pharmacological intervention. However, novel folds, low molarity and the need for lipid membrane support present serious challenges to the characterisation of their structure and interactions. We describe the use...
[NMR paper] Proton-Detected NMR Spectroscopy of Nanodisc-Embedded Membrane Proteins: MAS Solid-State vs. Solution-State Methods.
Proton-Detected NMR Spectroscopy of Nanodisc-Embedded Membrane Proteins: MAS Solid-State vs. Solution-State Methods.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles Proton-Detected NMR Spectroscopy of Nanodisc-Embedded Membrane Proteins: MAS Solid-State vs. Solution-State Methods.
J Phys Chem B. 2017 Jul 24;:
Authors: Lakomek NA, Frey L, Bibow S, Böckmann A, Riek R, Meier BH
Abstract
The structural and dynamical characterization of membrane proteins...
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07-25-2017 07:46 PM
[NMR paper] Applications of solid-state NMR to membrane proteins.
Applications of solid-state NMR to membrane proteins.
Applications of solid-state NMR to membrane proteins.
Biochim Biophys Acta. 2017 Jul 11;:
Authors: Ladizhansky V
Abstract
Membrane proteins mediate flow of molecules, signals, and energy between cells and intracellular compartments. Understanding membrane protein function requires a detailed understanding of the structural and dynamic properties involved. Lipid bilayers provide a native-like environment for structure-function investigations of membrane proteins. In this...
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07-16-2017 11:24 AM
Applications of solid-state NMR to membrane proteins
Applications of solid-state NMR to membrane proteins
Publication date: Available online 12 July 2017
Source:Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics</br>
Author(s): Vladimir Ladizhansky</br>
Membrane proteins mediate flow of molecules, signals, and energy between cells and intracellular compartments. Understanding membrane protein function requires a detailed understanding of the structural and dynamic properties involved. Lipid bilayers provide a native-like environment for structure-function investigations of membrane proteins. In this review...
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07-14-2017 01:32 AM
[NMR paper] Solid-state NMR methods for oriented membrane proteins.
Solid-state NMR methods for oriented membrane proteins.
Related Articles Solid-state NMR methods for oriented membrane proteins.
Prog Nucl Magn Reson Spectrosc. 2015 Aug;88-89:48-85
Authors: Hansen SK, Bertelsen K, Paaske B, Nielsen NC, Vosegaard T
Abstract
Oriented-sample solid-state NMR represents one of few experimental methods capable of characterising the membrane-bound conformation of proteins in the cell membrane. Since the technique was developed 25 years ago, the technique has been applied to study the structure of helix...
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08-19-2015 03:24 PM
Solid-state NMR methods for oriented membrane proteins
From The DNP-NMR Blog:
Solid-state NMR methods for oriented membrane proteins
This review article gives a comprehensive overview of the field of solid-state NMR spectroscopy on oriented samples. These experiments are typically less demanding on instrumentation since experiments are performed on static samples, without the need of spinning. While this article is not directly related to DNP-NMR spectroscopy, DNP-NMR on static samples will certainly become more interesting in the future.
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08-04-2015 01:43 AM
Solid-State NMR Methods for Oriented Membrane Proteins
Solid-State NMR Methods for Oriented Membrane Proteins
Publication date: Available online 19 May 2015
Source:Progress in Nuclear Magnetic Resonance Spectroscopy</br>
Author(s): Sara K. Hansen , Kresten Bertelsen , Berit Paaske , Niels Chr. Nielsen , Thomas Vosegaard</br>
Oriented-sample solid-state NMR represents one of few experimental methods capable of characterising the membrane-bound conformation of proteins in the cell membrane. Since the technique was developed 25 years ago, the technique has been applied to study the structure of helix bundle membrane...
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05-19-2015 09:10 AM
[NMR paper] Biomolecular magic-angle spinning solid-state NMR: recent methods and applications.
Biomolecular magic-angle spinning solid-state NMR: recent methods and applications.
Related Articles Biomolecular magic-angle spinning solid-state NMR: recent methods and applications.
Curr Opin Biotechnol. 2013 Mar 4;
Authors: Goldbourt A
Abstract
The link of structure and dynamics of biomolecules and their complexes to their function and to many cellular processes has driven the quest for their detailed characterization by a variety of biophysical techniques. Magic-angle spinning solid-state nuclear magnetic resonance spectroscopy...
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03-14-2013 10:05 PM
ICMRBS founder’s medal 2006: Biological solid-state NMR, methods and applications
ICMRBS founder’s medal 2006: Biological solid-state NMR, methods and applications
Marc Baldus
Journal of Biomolecular NMR; 2007; 39(1); pp 73-86
Abstract:
Solid-state NMR (ssNMR) provides increasing possibilities to study structure and dynamics of biomolecular systems. Our group has been interested in developing ssNMR-based approaches that are applicable to biomolecules of increasing molecular size and complexity without the need of specific isotope-labelling. Methodological aspects ranging from spectral assignments to the indirect detection of proton–proton contacts in...
Solid state NMR of membrane proteins: methods and applications
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