NMR paper Solid-state NMR and membrane proteins.

		BioNMR > Educational resources > Journal club
[NMR paper] Solid-state NMR and membrane proteins.

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

02-16-2015, 04:43 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,732

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Solid-state NMR and membrane proteins.

Solid-state NMR and membrane proteins.

Related Articles Solid-state NMR and membrane proteins.

J Magn Reson. 2014 Dec 29;

Authors: Opella SJ

Abstract
The native environment for a membrane protein is a phospholipid bilayer. Because the protein is immobilized on NMR timescales by the interactions within a bilayer membrane, solid-state NMR methods are essential to obtain high-resolution spectra. Approaches have been developed for both unoriented and oriented samples, however, they all rest on the foundation of the most fundamental aspects of solid-state NMR, and the chemical shift and homo- and hetero-nuclear dipole-dipole interactions. Solid-state NMR has advanced sufficiently to enable the structures of membrane proteins to be determined under near-native conditions in phospholipid bilayers.

PMID: 25681966 [PubMed - as supplied by publisher]

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] Solid-state NMR and Membrane Proteins Solid-state NMR and Membrane Proteins Publication date: Available online 29 December 2014 Source:Journal of Magnetic Resonance</br> Author(s): Stanley J. Opella</br> The native environment for a membrane protein is a phospholipid bilayer. Because the protein is immobilized on NMR timescales by the interactions within a bilayer membrane, solid-state NMR methods are essential to obtain high-resolution spectra. Approaches have been developed for both unoriented and oriented samples, however, they all rest on the foundation of the most fundamental aspects solid-state...	nmrlearner	Journal club	0	12-30-2014 03:28 PM
Perspectives in Enzymology of Membrane Proteins by Solid-State NMR From The DNP-NMR Blog: Perspectives in Enzymology of Membrane Proteins by Solid-State NMR Ullrich, S.J. and C. Glaubitz, Perspectives in Enzymology of Membrane Proteins by Solid-State NMR. Acc. Chem. Res., 2013. http://dx.doi.org/10.1021/ar4000289	nmrlearner	News from NMR blogs	0	08-12-2013 03:27 PM
[NMR paper] Perspectives in Enzymology of Membrane Proteins by Solid-State NMR. Perspectives in Enzymology of Membrane Proteins by Solid-State NMR. Related Articles Perspectives in Enzymology of Membrane Proteins by Solid-State NMR. Acc Chem Res. 2013 Jun 7; Authors: Ullrich SJ, Glaubitz C Abstract Membrane proteins catalyze reactions at the cell membrane and facilitate thetransport of molecules or signals across the membrane. Recently researchers have made great progress in understanding the structural biology of membrane proteins, mainly based on X-ray crystallography. In addition, the application of...	nmrlearner	Journal club	0	06-12-2013 11:42 AM
Proton-Detected Solid-State NMR Spectroscopy of Fibrillar and Membrane Proteins. Proton-Detected Solid-State NMR Spectroscopy of Fibrillar and Membrane Proteins. Proton-Detected Solid-State NMR Spectroscopy of Fibrillar and Membrane Proteins. Angew Chem Int Ed Engl. 2011 Apr 20; Authors: Linser R, Dasari M, Hiller M, Higman V, Fink U, Lopez Del Amo JM, Markovic S, Handel L, Kessler B, Schmieder P, Oesterhelt D, Oschkinat H, Reif B	nmrlearner	Journal club	0	04-22-2011 02:00 PM
Proton-Detected Solid-State NMR Spectroscopy of Fibrillar and Membrane Proteins. Proton-Detected Solid-State NMR Spectroscopy of Fibrillar and Membrane Proteins. Proton-Detected Solid-State NMR Spectroscopy of Fibrillar and Membrane Proteins. Angew Chem Int Ed Engl. 2011 Apr 14; Authors: Linser R, Dasari M, Hiller M, Higman V, Fink U, Lopez Del Amo JM, Markovic S, Handel L, Kessler B, Schmieder P, Oesterhelt D, Oschkinat H, Reif B	nmrlearner	Journal club	0	04-16-2011 12:29 PM
[NMR paper] How to prepare membrane proteins for solid-state NMR: A case study on the alpha-helical integral membrane protein diacylglycerol kinase from E. coli. How to prepare membrane proteins for solid-state NMR: A case study on the alpha-helical integral membrane protein diacylglycerol kinase from E. coli. Related Articles How to prepare membrane proteins for solid-state NMR: A case study on the alpha-helical integral membrane protein diacylglycerol kinase from E. coli. Chembiochem. 2005 Sep;6(9):1693-700 Authors: Lorch M, Faham S, Kaiser C, Weber I, Mason AJ, Bowie JU, Glaubitz C Several studies have demonstrated that it is viable to use microcrystalline preparations of water-soluble proteins as...	nmrlearner	Journal club	0	12-01-2010 06:56 PM
[NMR paper] Solid-state NMR spectroscopy applied to membrane proteins. Solid-state NMR spectroscopy applied to membrane proteins. Related Articles Solid-state NMR spectroscopy applied to membrane proteins. Curr Opin Struct Biol. 2000 Oct;10(5):593-600 Authors: de Groot HJ One major remaining problem in structural biology is to elucidate the structure and mechanism of function of membrane proteins. On the basis of preliminary information from genome projects, it is now estimated that up to 50,000 different membrane proteins may exist in the human being and that virtually every life process proceeds, sooner or...	nmrlearner	Journal club	0	11-19-2010 08:29 PM
Solid State NMR of membrane peptides and proteins Solid State NMR of membrane peptides and proteins Lecture notes on "Solid State NMR of membrane peptides and proteins" by Dr. SK Straus from Univ. of British Columbia More...	nmrlearner	General	0	08-16-2010 03:50 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off