Solid-state nuclear magnetic resonance (ssNMR) is a powerful technique for studying membrane protein structure and dynamics. Ideally, measurements are performed with the protein in a lipid bilayer. However, homogenous reconstitution of functional protein into intact bilayers at sufficiently high concentrations is often difficult to achieve. In this work, we investigate the suitability of the lipid cubic phase (LCP), which incorporates a lipid bilayer, as an alternative medium for ssNMR of...
[NMR paper] Probing and Manipulating the Lateral Pressure Profile in Lipid Bilayers Using Membrane-Active Peptides-A Solid-State 19F NMR Study
Probing and Manipulating the Lateral Pressure Profile in Lipid Bilayers Using Membrane-Active Peptides-A Solid-State 19F NMR Study
The lateral pressure profile constitutes an important physical property of lipid bilayers, influencing the binding, insertion, and function of membrane-active peptides, such as antimicrobial peptides. In this study, we demonstrate that the lateral pressure profile can be manipulated using the peptides residing in different regions of the bilayer. A ^(19)F-labeled analogue of the amphiphilic peptide PGLa was used to probe the lateral pressure at different depths...
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05-15-2022 06:54 AM
[NMR paper] Distinguishing Bicontinuous Lipid Cubic Phases from Isotropic Membrane Morphologies Using (31)P Solid-State NMR Spectroscopy.
Distinguishing Bicontinuous Lipid Cubic Phases from Isotropic Membrane Morphologies Using (31)P Solid-State NMR Spectroscopy.
Distinguishing Bicontinuous Lipid Cubic Phases from Isotropic Membrane Morphologies Using (31)P Solid-State NMR Spectroscopy.
J Phys Chem B. 2015 Mar 27;
Authors: Yang Y, Yao H, Hong M
Abstract
Nonlamellar lipid membranes are frequently induced by proteins that fuse, bend, and cut membranes. Understanding the mechanism of action of these proteins requires the elucidation of the membrane morphologies...
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03-31-2015 07:17 PM
[NMR paper] REDOR solid-state NMR as a probe of the membrane locations of membrane-associated peptides and proteins.
REDOR solid-state NMR as a probe of the membrane locations of membrane-associated peptides and proteins.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif REDOR solid-state NMR as a probe of the membrane locations of membrane-associated peptides and proteins.
J Magn Reson. 2015 Apr;253:154-65
Authors: Jia L, Liang S, Sackett K, Xie L, Ghosh U, Weliky DP
Abstract
Rotational-echo double-resonance (REDOR) solid-state NMR is applied to probe the membrane...
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03-24-2015 09:58 PM
REDOR solid-state NMR as a probe of the membrane locations of membrane-associated peptides and proteins
REDOR solid-state NMR as a probe of the membrane locations of membrane-associated peptides and proteins
Publication date: April 2015
Source:Journal of Magnetic Resonance, Volume 253</br>
Author(s): Lihui Jia , Shuang Liang , Kelly Sackett , Li Xie , Ujjayini Ghosh , David P. Weliky</br>
Rotational-echo double-resonance (REDOR) solid-state NMR is applied to probe the membrane locations of specific residues of membrane proteins. Couplings are measured between protein 13CO nuclei and membrane lipid or cholesterol 2H and 31P nuclei. Specific 13CO labeling is used...
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03-20-2015 01:48 AM
[NMR paper] Multiple Locations of Peptides in the Hydrocarbon Core of Gel-Phase Membranes Revealed by Peptide (13)C to Lipid (2)H REDOR Solid-State NMR.
Multiple Locations of Peptides in the Hydrocarbon Core of Gel-Phase Membranes Revealed by Peptide (13)C to Lipid (2)H REDOR Solid-State NMR.
Multiple Locations of Peptides in the Hydrocarbon Core of Gel-Phase Membranes Revealed by Peptide (13)C to Lipid (2)H REDOR Solid-State NMR.
Biochemistry. 2014 Dec 22;
Authors: Weliky DP
Abstract
Membrane locations of peptides and proteins are often critical to their functions. Solid-state rotational-echo double-resonance (REDOR) NMR is applied to probe the locations of two peptides via...
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12-23-2014 12:58 PM
Cryoprotection of lipid membranes for high-resolution solid-state NMR studies of membrane peptides and proteins at low temperature
From The DNP-NMR Blog:
Cryoprotection of lipid membranes for high-resolution solid-state NMR studies of membrane peptides and proteins at low temperature
Solid-state DNP-NMR are typically performed at cryogenic temperatures and samples, especially bio-macromolecules often require cryo-protection. This is a recent review about sample preparation and cryo-protecting samples to preserve the spectral resolution.
Lee, M. and M. Hong, Cryoprotection of lipid membranes for high-resolution solid-state NMR studies of membrane peptides and proteins at low temperature. J Biomol NMR, 2014....
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08-27-2014 02:29 PM
[NMR paper] Cryoprotection of lipid membranes for high-resolution solid-state NMR studies of membrane peptides and proteins at low temperature.
Cryoprotection of lipid membranes for high-resolution solid-state NMR studies of membrane peptides and proteins at low temperature.
Related Articles Cryoprotection of lipid membranes for high-resolution solid-state NMR studies of membrane peptides and proteins at low temperature.
J Biomol NMR. 2014 Jul 12;
Authors: Lee M, Hong M
Abstract
Solid-state NMR spectra of membrane proteins often show significant line broadening at cryogenic temperatures. Here we investigate the effects of several cryoprotectants to preserve the...
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07-13-2014 06:48 PM
Cryoprotection of lipid membranes for high-resolution solid-state NMR studies of membrane peptides and proteins at low temperature
Cryoprotection of lipid membranes for high-resolution solid-state NMR studies of membrane peptides and proteins at low temperature
Abstract
Solid-state NMR spectra of membrane proteins often show significant line broadening at cryogenic temperatures. Here we investigate the effects of several cryoprotectants to preserve the spectral resolution of lipid membranes and membrane peptides at temperatures down to ~200Â*K. Trehalose, glycerol, dimethylsulfoxide (DMSO), dimethylformamide (DMF), and polyethylene glycol (PEG), were chosen. These compounds are...
Solid-State NMR of Membrane Peptides and Proteins in the Lipid Cubic Phase
Linear Mode
