Related ArticlesA solid-state NMR index of helical membrane protein structure and topology.
J Magn Reson. 2000 May;144(1):150-5
Authors: Marassi FM, Opella SJ
The secondary structure and topology of membrane proteins can be described by inspection of two-dimensional (1)H-(15)N dipolar coupling/(15)N chemical shift polarization inversion spin exchange at the magic angle spectra obtained from uniformly (15)N-labeled samples in oriented bilayers. The characteristic wheel-like patterns of resonances observed in these spectra reflect helical wheel projections of residues in both transmembrane and in-plane helices and hence provide direct indices of the secondary structure and topology of membrane proteins in phospholipid bilayers. We refer to these patterns as PISA (polarity index slant angle) wheels. The transmembrane helix of the M2 peptide corresponding to the pore-lining segment of the acetylcholine receptor and the membrane surface helix of the antibiotic peptide magainin are used as examples.
Recent contributions from solid-state NMR to the understanding of membrane protein structure and function.
Recent contributions from solid-state NMR to the understanding of membrane protein structure and function.
Recent contributions from solid-state NMR to the understanding of membrane protein structure and function.
Curr Opin Chem Biol. 2011 Aug 19;
Authors: Judge PJ, Watts A
Abstract
The plasma membrane functions as a semi-permeable barrier, defining the interior (or cytoplasm) of an individual cell. This highly dynamic and complex macromolecular assembly comprises predominantly lipids and proteins held together by entropic forces and...
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[NMR paper] How to prepare membrane proteins for solid-state NMR: A case study on the alpha-helical integral membrane protein diacylglycerol kinase from E. coli.
How to prepare membrane proteins for solid-state NMR: A case study on the alpha-helical integral membrane protein diacylglycerol kinase from E. coli.
Related Articles How to prepare membrane proteins for solid-state NMR: A case study on the alpha-helical integral membrane protein diacylglycerol kinase from E. coli.
Chembiochem. 2005 Sep;6(9):1693-700
Authors: Lorch M, Faham S, Kaiser C, Weber I, Mason AJ, Bowie JU, Glaubitz C
Several studies have demonstrated that it is viable to use microcrystalline preparations of water-soluble proteins as...
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12-01-2010 06:56 PM
[NMR paper] Membrane protein structure determination using solid-state NMR.
Membrane protein structure determination using solid-state NMR.
Related Articles Membrane protein structure determination using solid-state NMR.
Methods Mol Biol. 2004;278:403-73
Authors: Watts A, Straus SK, Grage SL, Kamihira M, Lam YH, Zhao X
Solid-state NMR is emerging as a method for resolving structural information for large biomolecular complexes, such as membrane-embedded proteins. In principle, there is no molecular weight limit to the use of the approach, although the complexity and volume of data is still outside complete assignment...
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[NMR paper] Membrane protein topology probed by (1)H spin diffusion from lipids using solid-state
Membrane protein topology probed by (1)H spin diffusion from lipids using solid-state NMR spectroscopy.
Related Articles Membrane protein topology probed by (1)H spin diffusion from lipids using solid-state NMR spectroscopy.
J Am Chem Soc. 2002 Feb 6;124(5):874-83
Authors: Huster D, Yao X, Hong M
We describe a two-dimensional solid-state NMR technique to investigate membrane protein topology under magic-angle spinning conditions. The experiment detects the rate of (1)H spin diffusion from the mobile lipids to the rigid protein. While spin...
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11-24-2010 08:49 PM
[NMR paper] Membrane protein structure determination by solid state NMR.
Membrane protein structure determination by solid state NMR.
Related Articles Membrane protein structure determination by solid state NMR.
Nat Prod Rep. 1999 Aug;16(4):419-23
Authors: Watts A, Burnett IJ, Glaubitz C, Gröbner G, Middleton DA, Spooner PJ, Watts JA, Williamson PT
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11-18-2010 08:31 PM
[NMR paper] Membrane protein structure: the contribution and potential of novel solid state NMR a
Membrane protein structure: the contribution and potential of novel solid state NMR approaches.
Related Articles Membrane protein structure: the contribution and potential of novel solid state NMR approaches.
Mol Membr Biol. 1995 Jul-Sep;12(3):233-46
Authors: Watts A, Ulrich AS, Middleton DA
Alternative methods for describing molecular detail for large integral membrane proteins are required in the absence of routine crystallographic approaches. Novel solid state NMR methods, devised for the study of large molecular assemblies, are now finding...
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[NMR paper] Solid-state NMR approaches for studying membrane protein structure.
Solid-state NMR approaches for studying membrane protein structure.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--arjournals.annualreviews.org-images-AnnualReviews100x25.gif Related Articles Solid-state NMR approaches for studying membrane protein structure.
Annu Rev Biophys Biomol Struct. 1992;21:25-47
Authors: Smith SO, Peersen OB
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Probing membrane topology of the antimicrobial peptide distinctin by solid-state NMR
Probing membrane topology of the antimicrobial peptide distinctin by solid-state NMR spectroscopy in zwitterionic and charged lipid bilayers.
Related Articles Probing membrane topology of the antimicrobial peptide distinctin by solid-state NMR spectroscopy in zwitterionic and charged lipid bilayers.
Biochim Biophys Acta. 2010 Aug 15;
Authors: Verardi R, Traaseth NJ, Shi L, Porcelli F, Monfregola L, De Luca S, Amodeo P, Veglia G, Scaloni A
Distinctin is a 47-residue antimicrobial peptide, which interacts with negatively charged membranes and is...
A solid-state NMR index of helical membrane protein structure and topology.
Linear Mode
