Synucleinopathies are neurodegenerative diseases characterized by the accumulation of ?-synuclein protein aggregates in the neurons and glial cells. Both ex vivo and in vitro ?-synuclein fibrils tend to show polymorphism. Polymorphism results in structure variations among fibrils originating from a single polypeptide/protein. The polymorphs usually have different biophysical, biochemical and pathogenic properties. The various pathologies of a single disease might be associated with distinct...
[NMR paper] Membrane Interactions of alpha-Synuclein Revealed by Multiscale Molecular Dynamics Simulations, Markov State Models, and NMR
Membrane Interactions of alpha-Synuclein Revealed by Multiscale Molecular Dynamics Simulations, Markov State Models, and NMR
?-Synuclein (?S) is a presynaptic protein that binds to cell membranes and is linked to Parkinson's disease (PD). Binding of ?S to membranes is a likely first step in the molecular pathophysiology of PD. The ?S molecule can adopt multiple conformations, being largely disordered in water, adopting a ?-sheet conformation when present in amyloid fibrils, and forming a dynamic multiplicity of ?-helical conformations when bound to lipid bilayers and related...
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03-16-2021 06:18 AM
The Non-native Helical Intermediate State May Accumulateat Low pH in the Folding and Aggregation Landscape of the IntestinalFatty Acid Binding Protein
The Non-native Helical Intermediate State May Accumulateat Low pH in the Folding and Aggregation Landscape of the IntestinalFatty Acid Binding Protein
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.6b00390/20160803/images/medium/bi-2016-003902_0008.gif
Biochemistry
DOI: 10.1021/acs.biochem.6b00390
http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/bichaw/~4/Rud0gCwvf3A
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08-04-2016 11:21 PM
[NMR paper] Solid-state NMR sequential assignment of an Amyloid-?(1-42) fibril polymorph.
Solid-state NMR sequential assignment of an Amyloid-?(1-42) fibril polymorph.
Related Articles Solid-state NMR sequential assignment of an Amyloid-?(1-42) fibril polymorph.
Biomol NMR Assign. 2016 May 10;
Authors: Ravotti F, Wälti MA, Güntert P, Riek R, Böckmann A, Meier BH
Abstract
The formation of fibrils of the amyloid-? (A?) peptide is considered to be a key event in the pathology of Alzheimer's disease (AD). The determination of a high-resolution structure of these fibrils is relevant for the understanding of the molecular...
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05-12-2016 09:30 PM
[NMR paper] Further exploration of the conformational space of ?-synuclein fibrils: solid-state NMR assignment of a high-pH polymorph.
Further exploration of the conformational space of ?-synuclein fibrils: solid-state NMR assignment of a high-pH polymorph.
Related Articles Further exploration of the conformational space of ?-synuclein fibrils: solid-state NMR assignment of a high-pH polymorph.
Biomol NMR Assign. 2015 Aug 30;
Authors: Verasdonck J, Bousset L, Gath J, Melki R, Böckmann A, Meier BH
Abstract
Polymorphism is a common and important phenomenon for protein fibrils which has been linked to the appearance of strains in prion and other neurodegenerative...
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09-01-2015 10:48 AM
Dynamic nuclear polarization of a glassy matrix prepared by solid state mechanochemical amorphization of crystalline substances
From The DNP-NMR Blog:
Dynamic nuclear polarization of a glassy matrix prepared by solid state mechanochemical amorphization of crystalline substances
Elisei, E., et al., Dynamic nuclear polarization of a glassy matrix prepared by solid state mechanochemical amorphization of crystalline substances. Chem Commun (Camb), 2015. 51(11): p. 2080-3.
http://www.ncbi.nlm.nih.gov/pubmed/25534192
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04-09-2015 03:47 AM
Dynamic nuclear polarization of a glassy matrix prepared by solid state mechanochemical amorphization of crystalline substances
From The DNP-NMR Blog:
Dynamic nuclear polarization of a glassy matrix prepared by solid state mechanochemical amorphization of crystalline substances
This is a very interesting article describing the preparation of a sample for DNP by co-milling the analyte with the polarizing substrate. For a long time it was thought that just mechanically mixing the polarizing agent with the analyte will not result in a sample that is useful for DNP because the radical has to be mixed at an atomic level. Clearly this article demonstrate that this is not necessary. This will open up DNP to be used...
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02-09-2015 07:49 PM
[NMR paper] Temperature-dependent structural changes of Parkinson's alpha-synuclein reveal the role of pre-existing oligomers in alpha-synuclein fibrillization.
Temperature-dependent structural changes of Parkinson's alpha-synuclein reveal the role of pre-existing oligomers in alpha-synuclein fibrillization.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.plosone.org-images-pone_120x30.png http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles Temperature-dependent structural changes of Parkinson's alpha-synuclein reveal the role of pre-existing oligomers in alpha-synuclein fibrillization.
PLoS One. 2013;8(1):e53487
Authors: ...
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02-03-2013 10:19 AM
[NMR paper] How to prepare membrane proteins for solid-state NMR: A case study on the alpha-helical integral membrane protein diacylglycerol kinase from E. coli.
How to prepare membrane proteins for solid-state NMR: A case study on the alpha-helical integral membrane protein diacylglycerol kinase from E. coli.
Related Articles How to prepare membrane proteins for solid-state NMR: A case study on the alpha-helical integral membrane protein diacylglycerol kinase from E. coli.
Chembiochem. 2005 Sep;6(9):1693-700
Authors: Lorch M, Faham S, Kaiser C, Weber I, Mason AJ, Bowie JU, Glaubitz C
Several studies have demonstrated that it is viable to use microcrystalline preparations of water-soluble proteins as...
Solid-state NMR assignment of alpha-synuclein polymorph prepared from helical intermediate
Linear Mode
