[NMR paper] Solid-State NMR (31)P PRE Membrane Protein Immersion Depth Measurements.

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Solid-State NMR (31)P PRE Membrane Protein Immersion Depth Measurements.

Related Articles Solid-State NMR (31)P PRE Membrane Protein Immersion Depth Measurements.

J Phys Chem B. 2014 Apr 1;

Authors: Maltsev S, Hudson S, Sahu ID, Liu L, Lorigan GA

Abstract
Paramagnetic relaxation enhancement (PRE) is a widely used approach for measuring long-range distance constraints in biomolecular solution NMR spectroscopy. In this paper, we show that 31P PRE solid-state NMR spectroscopy can be utilized to determine the immersion depth of spin-labeled membrane peptides and proteins. Changes in the 31P NMR PRE times coupled with modeling studies can be used to describe the spin-label position/amino acid within the lipid bilayer and the corresponding helical tilt. This method provides valuable insight on protein-lipid interactions and membrane protein structural topology. Solid-state 31P NMR data on the 23 amino acid ?-helical nicotinic acetylcholine receptor nAChR M2? transmembrane domain model peptide followed predicted behavior of 31P PRE rates of the phospholipid headgroup as the spin label moves from the membrane surface towards the center of the membrane. Residue 11 showed the smallest changes in 31P PRE (center of the membrane), while residue 22 shows the largest 31P PRE change (near the membrane surface), when compared to the diamagnetic control M2? sample. This PRE SS-NMR technique can be used as a molecular ruler to measure membrane immersion depth.


PMID: 24689497 [PubMed - as supplied by publisher]



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