Solid-state 17O NMR as a probe for structural studies of proteins in biomembranes.
 

Solid-state 17O NMR as a probe for structural studies of proteins in biomembranes.

Related Articles Solid-state 17O NMR as a probe for structural studies of proteins in biomembranes.

J Am Chem Soc. 2004 Dec 1;126(47):15320-1

Authors: Lemaître V, de Planque MR, Howes AP, Smith ME, Dupree R, Watts A

We report the first example of 17O NMR spectra from a selectively labeled transmembrane peptide, 17O-[Ala12]-WALP23, as a lyophilized powder and incorporated in hydrated phospholipid vesicles. It is shown that at high magnetic field it is feasible to apply 17O NMR to the study of membrane-incorporated peptides. Furthermore, we were able to estimate distances within the selectively labeled WALP peptide, which represents a consensus transmembrane protein sequence. This work opens up new applications of 17O solid-state NMR on biological systems.

PMID: 15563125 [PubMed - indexed for MEDLINE]



Source: PubMed