Related ArticlesSolid-state 13C-NMR spectroscopy of adduction products of 2,5-hexanedione with ribonuclease, albumin, and rat neurofilament protein.
Chem Biol Interact. 1996 Oct 21;102(2):101-16
Authors: Yan B, DeCaprio AP, Zhu M, Bank S
The Paal-Knorr condensation reaction between the gamma-diketone 2,5-hexanedione (2,5-HD) and epsilon-amine moieties of proteins of various molecular weight, including ribonuclease (RNase), bovine serum albumin (BSA) and rat neurofilament (NF), has been investigated by solid-state 13C-NMR spectroscopy. These proteins all reacted with 2,5-HD with the formation of 2,5-dimethylpyrrole (2,5-DMP) derivatives. The size and complexity of the protein affected the rate of formation of 2,5-DMP derivatives. Using the selective reducing reagent NaCNBH3, the Paal-Knorr reaction intermediates were trapped by conversion into amines, which were identified by solid-state NMR spectroscopy. The secondary autoxidation reaction following the formation of 2,5-DMP derivatives was also studied by solid-state NMR spectroscopy.
Research Scientist, Solid State NMR Spectroscopy
Research Scientist, Solid State NMR Spectroscopy
A full time position is available for an experienced research scientist with skills in solid state NMR spectroscopy. This is a leading role in the University-wide NMR Facility, which has recently implemented ARC funding for a major initiative in solid-state NMR spectroscopy.
The appointee will consolidate advanced solid state NMR spectroscopic techniques to enable research in the areas of molecular & life sciences, medicine, materials engineering and polymers within UNSW and with partner organisations, as well as train research staff and...
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08-04-2011 01:20 PM
Solid-state NMR spectroscopy on complex biomolecules.
Solid-state NMR spectroscopy on complex biomolecules.
Solid-state NMR spectroscopy on complex biomolecules.
Angew Chem Int Ed Engl. 2010 Nov 2;49(45):8346-57
Authors: Renault M, Cukkemane A, Baldus M
Biomolecular applications of NMR spectroscopy are often merely associated with soluble molecules or magnetic resonance imaging. However, since the late 1970s, solid-state NMR (ssNMR) spectroscopy has demonstrated its ability to provide atomic-level insight into complex biomolecular systems ranging from lipid bilayers to complex biomaterials. In the...
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02-15-2011 07:17 PM
[NMR paper] Solid-state NMR spectroscopy applied to membrane proteins.
Solid-state NMR spectroscopy applied to membrane proteins.
Related Articles Solid-state NMR spectroscopy applied to membrane proteins.
Curr Opin Struct Biol. 2000 Oct;10(5):593-600
Authors: de Groot HJ
One major remaining problem in structural biology is to elucidate the structure and mechanism of function of membrane proteins. On the basis of preliminary information from genome projects, it is now estimated that up to 50,000 different membrane proteins may exist in the human being and that virtually every life process proceeds, sooner or...
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11-19-2010 08:29 PM
GFT projection NMR spectroscopy for proteins in the solid state.
GFT projection NMR spectroscopy for proteins in the solid state.
GFT projection NMR spectroscopy for proteins in the solid state.
J Biomol NMR. 2010 Oct 30;
Authors: Trent Franks W, Atreya HS, Szyperski T, Rienstra CM
Recording of four-dimensional (4D) spectra for proteins in the solid state has opened new avenues to obtain virtually complete resonance assignments and three-dimensional (3D) structures of proteins. As in solution state NMR, the sampling of three indirect dimensions leads per se to long minimal measurement time. Furthermore,...
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11-06-2010 11:02 AM
Supramolecular Interactions Probed by (13)C-(13)C Solid-State NMR Spectroscopy.
Supramolecular Interactions Probed by (13)C-(13)C Solid-State NMR Spectroscopy.
Related Articles Supramolecular Interactions Probed by (13)C-(13)C Solid-State NMR Spectroscopy.
J Am Chem Soc. 2010 Oct 8;
Authors: Loquet A, Giller K, Becker S, Lange A
We present a robust solid-state NMR approach for the accurate determination of molecular interfaces in insoluble and noncrystalline protein-protein complexes. The method relies on the measurement of intermolecular (13)C-(13)C distances in mixtures of glucose- and glucose-labeled proteins. We have...
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10-12-2010 02:52 PM
Supramolecular Interactions Probed by 13C-13C Solid-State NMR Spectroscopy
Supramolecular Interactions Probed by 13C-13C Solid-State NMR Spectroscopy
Antoine Loquet, Karin Giller, Stefan Becker and Adam Lange
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja107460j/aop/images/medium/ja-2010-07460j_0003.gif
Journal of the American Chemical Society
DOI: 10.1021/ja107460j
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/uejHS045F6M
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10-09-2010 03:03 AM
Trends in solid-state NMR spectroscopy and their relevance for bioanalytics.
Trends in solid-state NMR spectroscopy and their relevance for bioanalytics.
Related Articles Trends in solid-state NMR spectroscopy and their relevance for bioanalytics.
Anal Bioanal Chem. 2010 Aug 14;
Authors: Paasch S, Brunner E
Based on continuous methodical advances and developments, solid-state NMR spectroscopy has become a powerful tool for the investigation of various materials, including polymers, glasses, zeolites, fullerenes, and many others. During the past decade, solid-state NMR spectroscopy also found increasing interest for the...
Solid-state 13C-NMR spectroscopy of adduction products of 2,5-hexanedione with ribonu
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