Related ArticlesSoftware-assisted serum metabolite quantification using NMR.
Anal Chim Acta. 2016 Aug 31;934:194-202
Authors: Jung YS, Hyeon JS, Hwang GS
Abstract
The goal of metabolomics is to analyze a whole metabolome under a given set of conditions, and accurate and reliable quantitation of metabolites is crucial. Absolute concentration is more valuable than relative concentration; however, the most commonly used method in NMR-based serum metabolic profiling, bin-based and full data point peak quantification, provides relative concentration levels of metabolites and are not reliable when metabolite peaks overlap in a spectrum. In this study, we present the software-assisted serum metabolite quantification (SASMeQ) method, which allows us to identify and quantify metabolites in NMR spectra using Chenomx software. This software uses the ERETIC2 utility from TopSpin to add a digitally synthesized peak to a spectrum. The SASMeQ method will advance NMR-based serum metabolic profiling by providing an accurate and reliable method for absolute quantification that is superior to bin-based quantification.
[NMR paper] NMR quantification of trimethylamine-N-oxide in human serum and plasma in the clinical laboratory setting.
NMR quantification of trimethylamine-N-oxide in human serum and plasma in the clinical laboratory setting.
NMR quantification of trimethylamine-N-oxide in human serum and plasma in the clinical laboratory setting.
Clin Biochem. 2017 Jun 14;:
Authors: Garcia E, Wolak-Dinsmore J, Wang Z, Li XS, Bennett DW, Connelly MA, Otvos JD, Hazen SL, Jeyarajah EJ
Abstract
BACKGROUND AND OBJECTIVES: Trimethylamine-N-oxide (TMAO) produced by gut microbiota metabolism of dietary choline and carnitine has been shown to be associated with...
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06-19-2017 10:59 AM
[NMR paper] An LC-MS/MS based candidate reference method for the quantification of total gentamicin in human serum and plasma using NMR characterized calibrator material.
An LC-MS/MS based candidate reference method for the quantification of total gentamicin in human serum and plasma using NMR characterized calibrator material.
Related Articles An LC-MS/MS based candidate reference method for the quantification of total gentamicin in human serum and plasma using NMR characterized calibrator material.
Clin Chim Acta. 2016 Nov 18;:
Authors: Lucha S, Taibon J, Pongratz S, Geletneky C, Huber E, Wintterle-Roehm C, Lang R, Grimm SH, Duelffer T, Tarasov K, Zander J, Vogeser M, Kobold U
Abstract
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[NMR paper] Systematic identification of protein-metabolite interactions in complex metabolite mixtures by ligand-detected NMR spectroscopy.
Systematic identification of protein-metabolite interactions in complex metabolite mixtures by ligand-detected NMR spectroscopy.
Systematic identification of protein-metabolite interactions in complex metabolite mixtures by ligand-detected NMR spectroscopy.
Biochemistry. 2016 Apr 11;
Authors: Nikolaev YV, Kochanowski K, Link H, Sauer U, Allain FH
Abstract
Protein-metabolite interactions play a vital role in the regulation of numerous cellular processes. Consequently, identifying such interactions is a key prerequisite for...
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04-12-2016 09:12 PM
[NMR paper] A simple and accurate protocol for absolute polar metabolite quantification in cell cultures using q-NMR.
A simple and accurate protocol for absolute polar metabolite quantification in cell cultures using q-NMR.
Related Articles A simple and accurate protocol for absolute polar metabolite quantification in cell cultures using q-NMR.
Anal Biochem. 2016 Feb 17;
Authors: Goldoni L, Beringhelli T, Rocchia W, Realini N, Piomelli D
Abstract
Absolute analyte quantification by NMR spectroscopy is rarely pursued in metabolomics, even though this would allow researchers to compare results obtained using different techniques. Here we report on...
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02-24-2016 02:05 PM
[NMR paper] MOTOR: Model assisted software for NMR structure determination.
MOTOR: Model assisted software for NMR structure determination.
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Proteins. 2013 Jul 15;
Authors: Schieborr U, Sreeramulu S, Elshorst B, Maurer M, Saxena K, Stehle T, Kudlinzki D, Gande SL, Schwalbe H
Abstract
Eukaryotic proteins with important biological function can be partially unstructured, conformational flexible or heterogenic. Crystallization trials often fail for such proteins. In NMR spectroscopy, parts of the polypeptide chain undergoing dynamics in...
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07-16-2013 09:04 PM
[NMR paper] NMR identification of endogenous metabolites interacting with fatted and non-fatted human serum albumin in blood plasma: Fatty acids influence the HSA-metabolite interaction.
NMR identification of endogenous metabolites interacting with fatted and non-fatted human serum albumin in blood plasma: Fatty acids influence the HSA-metabolite interaction.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles NMR identification of endogenous metabolites interacting with fatted and non-fatted human serum albumin in blood plasma: Fatty acids influence the HSA-metabolite interaction.
J Magn Reson. 2013 Jan 8;228C:81-94
Authors: Jupin M, Michiels PJ, Girard FC, Spraul M,...
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NMR Identification of Endogenous Metabolites interacting with Fatted and Non-Fatted Human Serum Albumin in Blood Plasma: Fatty Acids influence the HSA-Metabolite Interaction
NMR Identification of Endogenous Metabolites interacting with Fatted and Non-Fatted Human Serum Albumin in Blood Plasma: Fatty Acids influence the HSA-Metabolite Interaction
Available online 8 January 2013
Publication year: 2013
Source:Journal of Magnetic Resonance</br>
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Metabolites and their concentrations are direct reporters on body biochemistry. Thanks to technical developments metabolic profiling of body fluids, such as blood plasma, by for instance NMR has in the past decade become increasingly accurate enabling successful clinical diagnostics. Human Serum...
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01-09-2013 10:01 AM
Comparison of serum metabolite compositions between obese and lean growing pigs using an NMR-based metabonomic approach.
Comparison of serum metabolite compositions between obese and lean growing pigs using an NMR-based metabonomic approach.
Comparison of serum metabolite compositions between obese and lean growing pigs using an NMR-based metabonomic approach.
J Nutr Biochem. 2011 Mar 21;
Authors: He Q, Ren P, Kong X, Wu Y, Wu G, Li P, Hao F, Tang H, Blachier F, Yin Y
Childhood obesity has become a prevalent risk to health of children and teenagers. To develop biomarkers in serum for altered lipid metabolism, genetically obese (Ningxiang strain) and lean...
Software-assisted serum metabolite quantification using NMR.
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