NMR paper Soft-pulsed aluminum-27 quadrupolar central transition NMR studies of ovotransferrin

		BioNMR > Educational resources > Journal club
[NMR paper] Soft-pulsed aluminum-27 quadrupolar central transition NMR studies of ovotransferrin

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

08-22-2010, 05:08 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,700

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Soft-pulsed aluminum-27 quadrupolar central transition NMR studies of ovotransferrin

Soft-pulsed aluminum-27 quadrupolar central transition NMR studies of ovotransferrin

Related Articles Soft-pulsed aluminum-27 quadrupolar central transition NMR studies of ovotransferrin

J Magn Reson. 1997 Dec;129(2):111-4

Authors: Aramini JM, Germann MW, Vogel HJ

We have employed soft (Gaussian) pulses to examine 27Al NMR signals arising from Al3+ bound to ovotransferrin. In addition to enhancing the general detectability of 27Al signals from a practical standpoint, this technique makes it possible to invert the central component of the protein-bound 27Al signals, providing a route for, to our knowledge, the first inversion-recovery measurements on such systems. The suitability of this approach for gaining insights into the nature of metal ion binding sites in large metalloproteins is critically assessed. Copyright 1997 Academic Press. Copyright 1997Academic Press

PMID: 9441874 [PubMed - as supplied by publisher]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Unequal quadrupolar splitting intensities Hi everyone I'm running an experiment looking at a mesoporous organosilica soaked in benzene-d6. The pores are tubular and aligned more or less in the same direction, so the anisotropic environment causes quadrupolar splitting when looking at deuterium. However, the splitting intensities are not equal: the left peak tends to be noticeably larger (~10-20%) than the right. Have tried this with the sample at different orientations in the field. At some angles, such at 180 degrees, the peaks are nearly equal in intensity, but at the rest of them the left is higher. So my question is: what...	sir_manning	NMR Questions and Answers	0	02-06-2012 06:33 PM
Quadrupole Central Transition (17)O NMR Spectroscopy of Biological Macromolecules in Aqueous Solution. Quadrupole Central Transition (17)O NMR Spectroscopy of Biological Macromolecules in Aqueous Solution. Quadrupole Central Transition (17)O NMR Spectroscopy of Biological Macromolecules in Aqueous Solution. J Am Chem Soc. 2010 Dec 22; Authors: Zhu J, Wu G We demonstrate a general nuclear magnetic resonance (NMR) spectroscopic approach in obtaining high-resolution (17)O (spin-5/2) NMR spectra for biological macromolecules in aqueous solution. This approach, termed quadrupole central transition (QCT) NMR, is based on the multiexponential relaxation...	nmrlearner	Journal club	0	12-24-2010 03:24 PM
Quadrupole Central Transition 17O NMR Spectroscopy of Biological Macromolecules in Aqueous Solution Quadrupole Central Transition 17O NMR Spectroscopy of Biological Macromolecules in Aqueous Solution Jianfeng Zhu and Gang Wu http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja1079207/aop/images/medium/ja-2010-079207_0005.gif Journal of the American Chemical Society DOI: 10.1021/ja1079207 http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/jacsat/~4/W23X0ykmsr4	nmrlearner	Journal club	0	12-23-2010 02:43 AM
[NMR paper] H-NMR and CD studies on the structural transition of serum albumin in the acidic regi H-NMR and CD studies on the structural transition of serum albumin in the acidic region--the N-->F transition. Related Articles H-NMR and CD studies on the structural transition of serum albumin in the acidic region--the N-->F transition. J Pept Res. 1998 Dec;52(6):431-42 Authors: Era S, Sogami M Helical content (f(alpha)) of bovine mercaptalbumin (BMA) showed the characteristic two-step decrease in the acidic region, one corresponding to the N-->F transition (pH 4.40-->3.75; f(alpha), 0.68-->0.58) and the other to the F-->E transition (the...	nmrlearner	Journal club	0	11-17-2010 11:15 PM
[NMR paper] Comparative 1H-NMR studies on the physical state of water in soft contact lens and mo Comparative 1H-NMR studies on the physical state of water in soft contact lens and mouse lens. Related Articles Comparative 1H-NMR studies on the physical state of water in soft contact lens and mouse lens. Biochim Biophys Acta. 1996 Apr 17;1289(3):369-76 Authors: Kuwata K, Era S, Sogami M, Amano H, Nagaoka S, Kato K, Takahashi K, Kitazawa Y, Watari H The physical state of water in mouse lenses (2-, 4- or 8-wk-old) and soft contact lenses (SCLs, water content from 18.4 to 79.2%) were studied by measuring spin-lattice relaxation times (T1) and...	nmrlearner	Journal club	0	08-22-2010 02:27 PM
[NMR paper] Thallium-205 and carbon-13 NMR studies of human sero- and chicken ovotransferrin. Thallium-205 and carbon-13 NMR studies of human sero- and chicken ovotransferrin. Related Articles Thallium-205 and carbon-13 NMR studies of human sero- and chicken ovotransferrin. Biochemistry. 1994 Mar 22;33(11):3304-11 Authors: Aramini JM, Krygsman PH, Vogel HJ We have examined the binding of Tl3+ to human serotransferrin and chicken ovotransferrin in the presence of carbonate and oxalate by 205Tl and 13C NMR spectroscopy. With carbonate as the synergistic anion, one observes two 205Tl NMR signals due to the bound metal ion in the two...	nmrlearner	Journal club	0	08-22-2010 03:33 AM
[NMR paper] Thallium-205 and carbon-13 NMR studies of human sero- and chicken ovotransferrin. Thallium-205 and carbon-13 NMR studies of human sero- and chicken ovotransferrin. Related Articles Thallium-205 and carbon-13 NMR studies of human sero- and chicken ovotransferrin. Biochemistry. 1994 Mar 22;33(11):3304-11 Authors: Aramini JM, Krygsman PH, Vogel HJ We have examined the binding of Tl3+ to human serotransferrin and chicken ovotransferrin in the presence of carbonate and oxalate by 205Tl and 13C NMR spectroscopy. With carbonate as the synergistic anion, one observes two 205Tl NMR signals due to the bound metal ion in the two...	nmrlearner	Journal club	0	08-22-2010 03:33 AM
[U. of Ottawa NMR Facility Blog] Solid State NMR of Half Integer Quadrupolar Nuclei Solid State NMR of Half Integer Quadrupolar Nuclei Many students who do liquid state NMR or solid state NMR of spin I=1/2 nuclei have very little appreciation for the information content and complexity of the solid state NMR spectra of spin I = n/2 quadrupolar nuclei (n= 3, 5, 7....). In part, I think this may be due to the mathematics involved with explaining the important effects. With this post, I attempt to describe the NMR spectrum of an I = 5/2 nucleus in the soild state without resorting to mathematics. I hope that this post helps to boost the understanding and appreciation for the...	nmrlearner	News from NMR blogs	0	08-21-2010 08:15 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off