One-dimensional (1D) proton-nuclear magnetic resonance (¹H-NMR) spectroscopy is an established technique for measuring small molecules in a wide variety of complex biological sample types. It is demonstrably reproducible, easily automatable and consequently ideal for routine and large-scale application. However, samples containing proteins, lipids, polysaccharides and other macromolecules produce broad signals which overlap and convolute those from small molecules. NMR experiment types designed...
Solvent signal suppression for high-resolution MAS-DNP #DNPNMR
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Solvent signal suppression for high-resolution MAS-DNP #DNPNMR
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Lee, D., S.R. Chaudhari, and G. De Paepe, Solvent signal suppression for high-resolution MAS-DNP. J Magn Reson, 2017. 278: p. 60-66.
https://www.ncbi.nlm.nih.gov/pubmed/28365491
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07-12-2017 02:16 PM
Understanding macromolecular crowding is critical for quantitative cell biology - SPIE Newsroom
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SPIE Newsroom
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Understanding macromolecular crowding is critical for quantitative cell biology
SPIE Newsroom
In the past, nuclear magnetic resonance (NMR) has been used to study protein-crowded environments and to investigate the translational and rotational diffusion of chymotrypsin inhibitor 2 in crowded environments. It was found that the heterogeneity of ...
Understanding macromolecular crowding is critical for...
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12-15-2015 07:47 AM
Understanding macromolecular crowding is critical for quantitative cell biology - SPIE Newsroom
http://www.bionmr.com//t1.gstatic.com/images?q=tbn:ANd9GcSFrZf31PUTVUTveakwA0E1nnJQ316UwMtUs4Y6ZDGqaBp0wkfIb8wo1Lsfi2ZHN1FLVa2FPOY
SPIE Newsroom
<img alt="" height="1" width="1">
Understanding macromolecular crowding is critical for quantitative cell biology
SPIE Newsroom
In the past, nuclear magnetic resonance (NMR) has been used to study protein-crowded environments and to investigate the translational and rotational diffusion of chymotrypsin inhibitor 2 in crowded environments. It was found that the heterogeneity of ...
Understanding macromolecular crowding is critical for...
nmrlearner
Online News
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12-12-2015 03:39 AM
Suppression of sampling artefacts in high-resolution four-dimensional NMR spectra using Signal Separation Algorithm
Suppression of sampling artefacts in high-resolution four-dimensional NMR spectra using Signal Separation Algorithm
Publication year: 2011
Source: Journal of Magnetic Resonance, Available online 20 October 2011</br>
Jan*Stanek, Rafal*Augustyniak, Wiktor*Ko?mi?ski</br>
The development of non-uniform sampling (NUS) strategies permits to obtain high-dimensional spectra with increased resolution in significantly reduced experimental time. We extended a previously proposed signal separation algorithm (SSA) to process sparse four-dimensional NMR data. It is employed for two experiments...
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Journal club
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10-22-2011 10:16 AM
Alternative SAIL-Trp for robust aromatic signal assignment and determination of the Ï?2 conformation by intra-residue NOEs
Alternative SAIL-Trp for robust aromatic signal assignment and determination of the Ï?2 conformation by intra-residue NOEs
Abstract Tryptophan (Trp) residues are frequently found in the hydrophobic cores of proteins, and therefore, their side-chain conformations, especially the precise locations of the bulky indole rings, are critical for determining structures by NMR. However, when analyzing -proteins, the observation and assignment of the ring signals are often hampered by excessive overlaps and tight spin couplings. These difficulties have been greatly alleviated by using...
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Journal club
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09-27-2011 07:04 AM
Ultrafast quantitative 2D NMR: an efficient tool for the measurement of specific isotopic enrichments in complex biological mixtures.
Ultrafast quantitative 2D NMR: an efficient tool for the measurement of specific isotopic enrichments in complex biological mixtures.
Ultrafast quantitative 2D NMR: an efficient tool for the measurement of specific isotopic enrichments in complex biological mixtures.
Anal Chem. 2011 Apr 15;83(8):3112-9
Authors: Giraudeau P, Massou S, Robin Y, Cahoreau E, Portais JC, Akoka S
Two-dimensional nuclear magnetic resonance (2D NMR) is a promising tool for studying metabolic fluxes by measuring (13)C-enrichments in complex mixtures of (13)C-labeled...
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08-04-2011 11:41 AM
Macromolecular NMR spectroscopy for the non-spectroscopist: beyond macromolecular solution structure determination.
Macromolecular NMR spectroscopy for the non-spectroscopist: beyond macromolecular solution structure determination.
Macromolecular NMR spectroscopy for the non-spectroscopist: beyond macromolecular solution structure determination.
FEBS J. 2011 Jan 7;
Authors: Bieri M, Kwan AH, Mobli M, King GF, Mackay JP, Gooley PR
A strength of NMR spectroscopy is its ability to monitor, on an atomic level, molecular changes and interactions. In this article, which is intended for non-spectroscopists, we describe major uses of NMR in protein science beyond...
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01-11-2011 11:27 PM
Solvent signal suppression in NMR
Solvent signal suppression in NMR
Publication year: 2010
Source: Progress in Nuclear Magnetic Resonance Spectroscopy, In Press, Accepted Manuscript, Available online 25 January 2010</br>
Gang, Zheng , William S., Price</br>
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SMolESY: an efficient and quantitative alternative to on-instrument macromolecular (1)H-NMR signal suppression
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