Small molecule induced toxic human-IAPP species characterized by NMR.
Chem Commun (Camb). 2020 Oct 02;:
Authors: Cox SJ, Rodriguez Camargo DC, Lee YH, Dubini RCA, Rovó P, Ivanova MI, Padmini V, Reif B, Ramamoorthy A
Abstract
In this study, the effect of CurDAc, a water-soluble curcumin derivative, on the formation and stability of amyloid fibers is revealed. CurDAc interaction with amyloid is structurally selective, which is reflected in a strong interference with hIAPP aggregation while showing weaker interactions with human-calcitonin and amyloid-?1-40 in comparison. Remarkably, CurDAc also exhibited potent fiber disaggregation for hIAPP generating a toxic oligomeric species.
PMID: 33006345 [PubMed - as supplied by publisher]
[NMR paper] Backbone NMR assignments of HypF-N under conditions generating toxic and non-toxic oligomers.
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Authors: Patel JR, Xu Y, Capitini C, Chiti F, De Simone A
Abstract
The HypF protein is involved in the maturation and regulation of hydrogenases. The N-terminal domain of HypF (HypF-N) has served as a key model system to study the pathways of protein amyloid formation and the nature of the...
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05-24-2018 12:57 AM
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Small Molecule and Peptide Recognition of ProteinTransmembrane Domains
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.6b00909/20170403/images/medium/bi-2016-009092_0009.gif
Biochemistry
DOI: 10.1021/acs.biochem.6b00909
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Nat Commun. 2014;5:5502
Authors: Luchinat E, Barbieri L, Rubino JT, Kozyreva T, Cantini F, Banci L
Abstract
Mutations in the superoxide dismutase 1 (SOD1) gene are related to familial cases of amyotrophic lateral sclerosis (fALS). Here we exploit in-cell NMR to characterize the protein folding and maturation of a series of fALS-linked SOD1 mutants in human cells and to...
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Methods Mol Biol. 2013;1008:389-413
Authors: Davis B
Abstract
Nuclear magnetic resonance (NMR) is well suited to probing the interactions between ligands and macromolecular receptors. It is a truly label-free technique, requiring only the presence of atoms (usually (1)H or (19)F) which give rise to observable resonances on either the ligand or the receptor. A number of parameters associated with these resonances can...
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[NMR paper] NMR Methods for Detection of Small Molecule Binding to RGS4.
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Authors: Storaska AJ, Neubig RR
Abstract
The duration and amplitude of G-protein-coupled receptor (GPCR) signaling is controlled by regulator of G-protein signaling (RGS) proteins. The 20 RGS family members act as GTPase accelerating proteins through their interaction with the G? subunit of the G??? heterotrimer. Their influence over GPCR signaling has attracted many to...
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Small molecule induced toxic human-IAPP species characterized by NMR.
Linear Mode
