The small GTPases Ras and Rheb studied by multidimensional NMR spectroscopy: structure and function.
Biol Chem. 2017 May 01;398(5-6):577-588
Authors: Schöpel M, Potheraveedu VN, Al-Harthy T, Abdel-Jalil R, Heumann R, Stoll R
Abstract
Ras GTPases are key players in cellular signalling because they act as binary switches. These states manifest through toggling between an active (GTP-loaded) and an inactive (GDP-loaded) form. The hydrolysis and replenishing of GTP is controlled by two additional protein classes: GAP (GTPase-activating)- and GEF (Guanine nucleotide exchange factors)-proteins. The complex interplay of the proteins is known as the GTPase-cycle. Several point mutations of the Ras protein deregulate this cycle. Mutations in Ras are associated with up to one-third of human cancers. The three isoforms of Ras (H, N, K) exhibit high sequence similarity and mainly differ in a region called HVR (hypervariable region). The HVR governs the differential action and cellular distribution of the three isoforms. Rheb is a Ras-like GTPase that is conserved from yeast to mammals. Rheb is mainly involved in activation of cell growth through stimulation of mTORC1 activity. In this review, we summarise multidimensional NMR studies on Rheb and Ras carried out to characterise their structure-function relationship and explain how the activity of these small GTPases can be modulated by low molecular weight compounds. These might help to design GTPase-selective antagonists for treatment of cancer and brain disease.
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Biochim Biophys Acta. 2014 Dec 9;
Authors: Bodor A, Kövér KE, Mäler L
Abstract
Small fast-tumbling bicelles are ideal for studies of membrane interactions at molecular level; they allow analysis of lipid properties using solution-state NMR. In the present study we used (31)P NMR relaxation to obtain detailed information on lipid head-group dynamics. We explored the effect...
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Abstract
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Publication year: 2010
Source:Progress in Nuclear Magnetic Resonance Spectroscopy, Volume 57, Issue 2</br>
Joseph A. Goncalves, Shivani Ahuja, Sina Erfani, Markus Eilers, Steven O. Smith</br>
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Publication year: 2010
Source: Progress in Nuclear Magnetic Resonance Spectroscopy, In Press, Accepted Manuscript, Available online 12 May 2010</br>
Joseph A., Goncalves , Shivani, Ahuja , Sina, Erfani , Markus, Eilers , Steven O., Smith</br>
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The small GTPases Ras and Rheb studied by multidimensional NMR spectroscopy: structure and function.
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