NMR paper Slow global motions in biosolids studied by the deuteron stimulated echo NMR experiment

		BioNMR > Educational resources > Journal club
[NMR paper] Slow global motions in biosolids studied by the deuteron stimulated echo NMR experiment

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

11-13-2024, 07:00 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Slow global motions in biosolids studied by the deuteron stimulated echo NMR experiment

Slow global motions in biosolids studied by the deuteron stimulated echo NMR experiment

Recent 15N R1?-relaxation studies have shown that proteins in the solid state undergo slow, low amplitude global motion in the sub-millisecond time range. This range is at the edge of the time window for R1? experiments and, therefore, the motional parameters obtained by this method are not precise or reliable. In this paper, we present a 2H stimulated echo study of this type of molecular dynamics. The 2H stimulated echo experiments on a static sample allow for direct measurement of the...

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Recent developments in deuterium solid-state NMR for the detection of slow motions in proteins - ScienceDirect Recent developments in deuterium solid-state NMR for the detection of slow motions in proteins - ScienceDirect Recent developments in deuterium solid-state NMR for the detection of slow motions in proteins ScienceDirect Read here	nmrlearner	Online News	0	02-17-2024 12:31 AM
[NMR paper] Recent developments in deuterium solid-state NMR for the detection of slow motions in proteins. Recent developments in deuterium solid-state NMR for the detection of slow motions in proteins. Related Articles Recent developments in deuterium solid-state NMR for the detection of slow motions in proteins. Solid State Nucl Magn Reson. 2021 Jan 07;111:101710 Authors: Vugmeyster L Abstract Slow timescale dynamics in proteins are essential for a variety of biological functions spanning ligand binding, enzymatic catalysis, protein folding and misfolding regulations, as well as protein-protein and protein-nucleic acid interactions....	nmrlearner	Journal club	0	01-16-2021 04:55 PM
[NMR paper] Slow motions in microcrystalline proteins as observed by MAS-dependent (15)N rotating-frame NMR relaxation. Slow motions in microcrystalline proteins as observed by MAS-dependent (15)N rotating-frame NMR relaxation. Related Articles Slow motions in microcrystalline proteins as observed by MAS-dependent (15)N rotating-frame NMR relaxation. J Magn Reson. 2014 Sep 20;248C:8-12 Authors: Krushelnitsky A, Zinkevich T, Reif B, Saalwächter K Abstract (15)N NMR relaxation rate R1? measurements reveal that a substantial fraction of residues in the microcrystalline chicken alpha-spectrin SH3 domain protein undergoes dynamics in the ?s-ms...	nmrlearner	Journal club	0	10-06-2014 12:37 PM
[NMR paper] Slow motions in microcrystalline proteins as observed by MAS-dependent 15N rotating-frame NMR relaxation Slow motions in microcrystalline proteins as observed by MAS-dependent 15N rotating-frame NMR relaxation Publication date: Available online 20 September 2014 Source:Journal of Magnetic Resonance</br> Author(s): Alexey Krushelnitsky , Tatiana Zinkevich , Bernd Reif , Kay Saalwächter</br> 15N NMR relaxation rate R 1? measurements reveal that a substantial fraction of residues in the microcrystalline chicken alpha-spectrin SH3 domain protein undergoes dynamics in the ?s - ms timescale range. On the basis of a comparison of 2D site-resolved with 1D integrated 15N...	nmrlearner	Journal club	0	09-20-2014 07:51 PM
[NMR paper] Identification of slow motions in the reduced recombinant high-potential iron sulfur Identification of slow motions in the reduced recombinant high-potential iron sulfur protein I (HiPIP I) from Ectothiorhodospira halophila via 15N rotating-frame NMR relaxation measurements. Related Articles Identification of slow motions in the reduced recombinant high-potential iron sulfur protein I (HiPIP I) from Ectothiorhodospira halophila via 15N rotating-frame NMR relaxation measurements. J Biomol NMR. 1998 Aug;12(2):307-18 Authors: Banci L, Felli IC, Koulougliotis D Rotating-frame 15N relaxation rate (R1 rho) NMR experiments have been...	nmrlearner	Journal club	0	11-17-2010 11:15 PM
[NMR paper] NMR evidence for slow collective motions in cyanometmyoglobin. NMR evidence for slow collective motions in cyanometmyoglobin. Related Articles NMR evidence for slow collective motions in cyanometmyoglobin. Nat Struct Biol. 1997 Apr;4(4):292-7 Authors: Tolman JR, Flanagan JM, Kennedy MA, Prestegard JH Residual dipolar couplings observed in NMR spectra at very high magnetic fields have been measured to a high degree of accuracy for the paramagnetic protein cyanometmyoglobin. Deviations of these measurements from predictions based on available crystallographic and solution structures are largely systematic...	nmrlearner	Journal club	0	08-22-2010 03:31 PM
[NMR paper] NMR evidence for slow collective motions in cyanometmyoglobin. NMR evidence for slow collective motions in cyanometmyoglobin. Related Articles NMR evidence for slow collective motions in cyanometmyoglobin. Nat Struct Biol. 1997 Apr;4(4):292-7 Authors: Tolman JR, Flanagan JM, Kennedy MA, Prestegard JH Residual dipolar couplings observed in NMR spectra at very high magnetic fields have been measured to a high degree of accuracy for the paramagnetic protein cyanometmyoglobin. Deviations of these measurements from predictions based on available crystallographic and solution structures are largely systematic...	nmrlearner	Journal club	0	08-22-2010 03:03 PM
Self-diffusion in Polymer Systems studied by Magnetic Field-Gradient Spin-Echo NMR Me Self-diffusion in Polymer Systems studied by Magnetic Field-Gradient Spin-Echo NMR Methods Publication year: 2010 Source: Progress in Nuclear Magnetic Resonance Spectroscopy, In Press, Accepted Manuscript, Available online 13 April 2010</br> Harald, Walderhaug , Olle, Söderman , Daniel, Topgaard</br> More...	nmrlearner	Journal club	0	08-16-2010 03:50 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off