In situ NMR measurement of macromolecule-bound metal ion concentrations.
In situ NMR measurement of macromolecule-bound metal ion concentrations.
J Biomol NMR. 2016 Apr 23;
Authors: Kozlyuk N, Sengupta S, Lupták A, Martin RW
Abstract
Many nucleic acids and proteins require divalent metal ions such as Mg(2+) and Ca(2+) for folding and function. The lipophilic alignment media frequently used as membrane mimetics also bind these divalent metals. Here we demonstrate the use of (31)P NMR spectrum of a metal ion chelator (deoxycytidine diphosphate) to measure the bound [Mg(2+)] and [Ca(2+)] in situ for several biological model systems at relatively high divalent ion concentrations (1-10*mM). This method represents a general approach to measuring divalent metal ion binding in NMR samples where the amount and type of metal ion added to the system is known.
PMID: 27108020 [PubMed - as supplied by publisher]
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