Proton exchange is a fundamental chemical event, and NMR provides the most direct readout of protonation events with site-specific resolution. Conventional approaches require manual titration of sample pH to collect a series of NMR spectra at different pH values. This requires extensive sample handling and often results in significant sample loss, leading to reduced signal or the need to prepare additional samples. Here, we introduce a novel approach to control pH in NMR samples using water...
PrimaryTransfer Step in the Light-Driven Ion PumpBacteriorhodopsin: An Irreversible U-Turn Revealed by DynamicNuclear Polarization-Enhanced Magic Angle Spinning NMR
PrimaryTransfer Step in the Light-Driven Ion PumpBacteriorhodopsin: An Irreversible U-Turn Revealed by DynamicNuclear Polarization-Enhanced Magic Angle Spinning NMR
Qing Zhe Ni, Thach V. Can, Eugenio Daviso, Marina Belenky, Robert G. Griffin and Judith Herzfeld
https://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/jacs.8b00022/20180312/images/medium/ja-2018-00022t_0006.gif
Journal of the American Chemical Society
DOI: 10.1021/jacs.8b00022
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA...
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03-13-2018 04:24 AM
500-fold enhancement of in situ (13)C liquid state NMR using gyrotron-driven temperature-jump DNP #DNPNMR
From The DNP-NMR Blog:
500-fold enhancement of in situ (13)C liquid state NMR using gyrotron-driven temperature-jump DNP #DNPNMR
Yoon, D., et al., 500-fold enhancement of in situ (13)C liquid state NMR using gyrotron-driven temperature-jump DNP. J Magn Reson, 2016. 270: p. 142-6.
http://www.ncbi.nlm.nih.gov/pubmed/27490302
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09-02-2016 11:54 PM
[NMR paper] Proton-Detected 2D Radio Frequency Driven Recoupling Solid-state NMR Studies on Micelle-associated Cytochrome-b5
Proton-Detected 2D Radio Frequency Driven Recoupling Solid-state NMR Studies on Micelle-associated Cytochrome-b5
Publication date: Available online 1 March 2014
Source:Journal of Magnetic Resonance</br>
Author(s): Manoj Kumar Pandey , Subramanian Vivekanandan , Kazutoshi Yamamoto , Sangchoul Im , Lucy Waskell , Ayyalusamy Ramamoorthy</br>
Solid-state NMR spectroscopy is increasingly used in the high-resolution structural studies of membrane-associated proteins and peptides. Most such studies necessitate isotopically labeled (13C, 15N and 2H) proteins/peptides,...
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03-02-2014 01:53 AM
A systematic mutagenesis-driven strategy for site-resolved NMR studies of supramolecular assemblies
A systematic mutagenesis-driven strategy for site-resolved NMR studies of supramolecular assemblies
Abstract Obtaining sequence-specific assignments remains a major bottleneck in solution NMR investigations of supramolecular structure, dynamics and interactions. Here we demonstrate that resonance assignment of methyl probes in high molecular weight protein assemblies can be efficiently achieved by combining fast NMR experiments, residue-type-specific isotope-labeling and automated site-directed mutagenesis. The utility of this general and straightforward strategy is demonstrated through...
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06-06-2011 12:53 AM
A systematic mutagenesis-driven strategy for site-resolved NMR studies of supramolecular assemblies.
A systematic mutagenesis-driven strategy for site-resolved NMR studies of supramolecular assemblies.
A systematic mutagenesis-driven strategy for site-resolved NMR studies of supramolecular assemblies.
J Biomol NMR. 2011 May 29;
Authors: Amero C, Asunción Durá M, Noirclerc-Savoye M, Perollier A, Gallet B, Plevin MJ, Vernet T, Franzetti B, Boisbouvier J
Obtaining sequence-specific assignments remains a major bottleneck in solution NMR investigations of supramolecular structure, dynamics and interactions. Here we demonstrate that resonance...