Site-specific protein methyl deuterium quadrupolar patterns by proton-detected 3D (2)H-(13)C-(1)H MAS NMR spectroscopy
 

Site-specific protein methyl deuterium quadrupolar patterns by proton-detected 3D (2)H-(13)C-(1)H MAS NMR spectroscopy

Determination of protein structure and dynamics is key to understand the mechanism of protein action. Perdeuterated proteins have been used to obtain high resolution/sensitivty NMR experiments via proton-detection. These methods utilizes ¹H, ^(13)C and ^(15)N nuclei for chemical shift dispersion or relaxation probes, despite the existing abundant deuterons. However, a high-sensitivity NMR method to utilize deuterons and e.g. determine site-specific deuterium quadrupolar pattern information has...

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