Related ArticlesSite-specific interaction between ?-synuclein and membranes probed by NMR-observed methionine oxidation rates.
J Am Chem Soc. 2013 Feb 11;
Authors: Maltsev AS, Chen J, Levine RL, Bax A
Abstract
?-Synuclein (aS) is an intrinsically disordered protein that is water soluble but also can bind negatively charged lipid membranes while adopting an ?-helical conformation. Membrane affinity is increased by post-translational N-terminal acetylation, a common modification in all eukaroytic cells. In the presence of lipid vesicles containing a small fraction of peroxidized lipids, the N-terminal Met residues in aS (Met1 and Met5) rapidly oxidize while reducing the toxic lipid hydroperoxide to a non-reactive lipid hydroxide, whereas C-terminal Met residues remain unaffected. Met oxidation can be probed conveniently and quantitatively by NMR spectroscopy. Results show that oxidation of Met1 reduces the oxidation rate of Met5, and vice versa, caused by decreased membrane affinity of the partially oxidized protein. The effect of Met oxidation on aS-membrane affinity extends over large distances, with the oxidation rate of Met49 in the V49M mutant of aS being strongly impacted by oxidation states of Met1 and Met5, and inversely the oxidation state of Met49 affecting the oxidation rates of the N-terminal Met residues. When not bound to membrane, oxidized Met1 and Met5 of aS are excellent substrates for methionine sulfoxide reductase (Msr), thereby providing an efficient vehicle for water-soluble Msr enzymes to protect the membrane against oxidative damage.
PMID: 23398174 [PubMed - as supplied by publisher]
[NMR paper] Copper(I)-?-Synuclein Interaction: Structural Description of Two Independent and Competing Metal Binding Sites.
Copper(I)-?-Synuclein Interaction: Structural Description of Two Independent and Competing Metal Binding Sites.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles Copper(I)-?-Synuclein Interaction: Structural Description of Two Independent and Competing Metal Binding Sites.
Inorg Chem. 2013 Jan 23;
Authors: Camponeschi F, Valensin D, Tessari I, Bubacco L, Dell'acqua S, Casella L, Monzani E, Gaggelli E, Valensin G
Abstract
The aggregation of ?-synuclein (?S) is a critical step in...
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Specific 12C?D212C?D2S13C?HD2 IsotopomerLabeling of Methionine ToCharacterize Protein Dynamics by 1H and 13CNMR Relaxation Dispersion
Specific 12C?D212C?D2S13C?HD2 IsotopomerLabeling of Methionine ToCharacterize Protein Dynamics by 1H and 13CNMR Relaxation Dispersion
Ulrich Weininger, Zhihong Liu, Deane D. McIntyre, Hans J. Vogel and Mikael Akke
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja309294u/aop/images/medium/ja-2012-09294u_0005.gif
Journal of the American Chemical Society
DOI: 10.1021/ja309294u
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/9TseQlKMjfM
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11-03-2012 12:26 AM
[NMR paper] Interaction of the replication terminator protein of Bacillus subtilis with DNA probed by NMR spectroscopy.
Interaction of the replication terminator protein of Bacillus subtilis with DNA probed by NMR spectroscopy.
Related Articles Interaction of the replication terminator protein of Bacillus subtilis with DNA probed by NMR spectroscopy.
Biochem Biophys Res Commun. 2005 Sep 23;335(2):361-6
Authors: Hastings AF, Otting G, Folmer RH, Duggin IG, Wake RG, Wilce MC, Wilce JA
Termination of DNA replication in Bacillus subtilis involves the polar arrest of replication forks by a specific complex formed between the dimeric 29 kDa replication terminator...
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[NMR paper] Interaction of the fusogenic peptide B18 in its amyloid-state with lipid membranes st
Interaction of the fusogenic peptide B18 in its amyloid-state with lipid membranes studied by solid state NMR.
Related Articles Interaction of the fusogenic peptide B18 in its amyloid-state with lipid membranes studied by solid state NMR.
Chem Phys Lipids. 2004 Nov;132(1):65-77
Authors: Grage SL, Afonin S, Grüne M, Ulrich AS
The interaction of the fusogenic polypeptide segment "B18" from the fertilization protein binding with lipid membranes was investigated by solid state 2H and 31P NMR, and by differential scanning calorimetry. B18 is known...
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11-24-2010 10:03 PM
Protein-ice interaction of an antifreeze protein observed with solid-state NMR [Chemi
Protein-ice interaction of an antifreeze protein observed with solid-state NMR
Siemer, A. B., Huang, K.-Y., McDermott, A. E....
Date: 2010-10-12
NMR on frozen solutions is an ideal method to study fundamental questions of macromolecular hydration, because the hydration shell of many biomolecules does not freeze together with bulk solvent. In the present study, we present previously undescribed NMR methods to study the interactions of proteins with their hydration shell and the ice lattice in frozen solution. We applied these methods to compare solvent interaction of an ice-binding...
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Protein-ice interaction of an antifreeze protein observed with solid-state NMR.
Protein-ice interaction of an antifreeze protein observed with solid-state NMR.
Related Articles Protein-ice interaction of an antifreeze protein observed with solid-state NMR.
Proc Natl Acad Sci U S A. 2010 Sep 30;
Authors: Siemer AB, Huang KY, McDermott AE
NMR on frozen solutions is an ideal method to study fundamental questions of macromolecular hydration, because the hydration shell of many biomolecules does not freeze together with bulk solvent. In the present study, we present previously undescribed NMR methods to study the interactions...
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[NMR paper] Resolution of individual lipids in mixed phospholipid membranes and specific lipid-cy
Resolution of individual lipids in mixed phospholipid membranes and specific lipid-cytochrome c interactions by magic-angle spinning solid-state phosphorus-31 NMR.
Related Articles Resolution of individual lipids in mixed phospholipid membranes and specific lipid-cytochrome c interactions by magic-angle spinning solid-state phosphorus-31 NMR.
Biochemistry. 1994 Mar 8;33(9):2459-67
Authors: Pinheiro TJ, Watts A
A model of the inner mitochondrial membrane was constructed with dioleoyphosphatidylcholine (PC), dioleoylphosphatidylethanolamine...
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[NMR paper] Resolution of individual lipids in mixed phospholipid membranes and specific lipid-cy
Resolution of individual lipids in mixed phospholipid membranes and specific lipid-cytochrome c interactions by magic-angle spinning solid-state phosphorus-31 NMR.
Related Articles Resolution of individual lipids in mixed phospholipid membranes and specific lipid-cytochrome c interactions by magic-angle spinning solid-state phosphorus-31 NMR.
Biochemistry. 1994 Mar 8;33(9):2459-67
Authors: Pinheiro TJ, Watts A
A model of the inner mitochondrial membrane was constructed with dioleoyphosphatidylcholine (PC), dioleoylphosphatidylethanolamine...