Related ArticlesSite-specific identification of an a? fibril-heparin interaction site by using solid-state NMR spectroscopy.
Angew Chem Int Ed Engl. 2012 Dec 21;51(52):13140-3
Authors: Madine J, Pandya MJ, Hicks MR, Rodger A, Yates EA, Radford SE, Middleton DA
Abstract
At the surface of A?(1-40) amyloid fibrils that have a threefold molecular symmetry (green in the left picture) a site of interaction of the glycosaminoglycan analogue heparin (blue) was identified. The binding site consists of residues at the N terminus and the turn regions defining the apices of the triangular geometry. Heparin has a lower affinity for A?(1-40) fibrils having twofold molecular symmetry, thus revealing a remarkable morphological selectivity.
[NMR paper] Probing site-specific (13)C/ (15)N-isotope enrichment of spider silk with liquid-state NMR spectroscopy.
Probing site-specific (13)C/ (15)N-isotope enrichment of spider silk with liquid-state NMR spectroscopy.
Probing site-specific (13)C/ (15)N-isotope enrichment of spider silk with liquid-state NMR spectroscopy.
Anal Bioanal Chem. 2013 Feb 26;
Authors: Shi X, Yarger JL, Holland GP
Abstract
Solid-state nuclear magnetic resonance (NMR) has been extensively used to elucidate spider silk protein structure and dynamics. In many of these studies, site-specific isotope enrichment is critical for designing particular NMR methods...
nmrlearner
Journal club
0
02-26-2013 06:35 PM
[NMR paper] Site-specific interaction between ?-synuclein and membranes probed by NMR-observed methionine oxidation rates.
Site-specific interaction between ?-synuclein and membranes probed by NMR-observed methionine oxidation rates.
Related Articles Site-specific interaction between ?-synuclein and membranes probed by NMR-observed methionine oxidation rates.
J Am Chem Soc. 2013 Feb 11;
Authors: Maltsev AS, Chen J, Levine RL, Bax A
Abstract
?-Synuclein (aS) is an intrinsically disordered protein that is water soluble but also can bind negatively charged lipid membranes while adopting an ?-helical conformation. Membrane affinity is increased by...
nmrlearner
Journal club
0
02-13-2013 12:47 PM
[NMR paper] Asymmetry of 13C labeled 3-pyruvate affords improved site specific labeling of RNA for NMR spectroscopy.
From Mendeley Biomolecular NMR group:
Asymmetry of 13C labeled 3-pyruvate affords improved site specific labeling of RNA for NMR spectroscopy.
Journal of biomolecular NMR (2012). Volume: 52, Issue: 1. Pages: 65-77. Chandar S Thakur, T Kwaku Dayie et al.
Selective isotopic labeling provides an unparalleled window within which to study the structure and dynamics of RNAs by high resolution NMR spectroscopy. Unlike commonly used carbon sources, the asymmetry of (13)C-labeled pyruvate provides selective labeling in both the ribose and base moieties of nucleotides using Escherichia coli...
nmrlearner
Journal club
0
10-12-2012 09:58 AM
Site-Resolved Measurementof Microsecond-to-MillisecondConformational-Exchange Processes in Proteins by Solid-State NMR Spectroscopy
Site-Resolved Measurementof Microsecond-to-MillisecondConformational-Exchange Processes in Proteins by Solid-State NMR Spectroscopy
Martin Tollinger, Astrid C. Sivertsen, Beat H. Meier, Matthias Ernst and Paul Schanda
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja303591y/aop/images/medium/ja-2012-03591y_0005.gif
Journal of the American Chemical Society
DOI: 10.1021/ja303591y
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/ZVmFwVkbuRs
nmrlearner
Journal club
0
08-29-2012 04:28 AM
Site-Specific Solid-State NMR Detection of Hydrogen-Deuterium Exchange Reveals Conformational Changes in a 7-Helical Transmembrane Protein.
Site-Specific Solid-State NMR Detection of Hydrogen-Deuterium Exchange Reveals Conformational Changes in a 7-Helical Transmembrane Protein.
Site-Specific Solid-State NMR Detection of Hydrogen-Deuterium Exchange Reveals Conformational Changes in a 7-Helical Transmembrane Protein.
Biophys J. 2011 Aug 3;101(3):L23-L25
Authors: Wang S, Shi L, Kawamura I, Brown LS, Ladizhansky V
Solid-state NMR spectroscopy is an efficient tool for following conformational dynamics of membrane proteins at atomic resolution. We used this technique for the site-specific...
nmrlearner
Journal club
0
08-03-2011 12:00 PM
[NMR paper] Probing site-specific conformational distributions in protein folding with solid-stat
Probing site-specific conformational distributions in protein folding with solid-state NMR.
Related Articles Probing site-specific conformational distributions in protein folding with solid-state NMR.
Proc Natl Acad Sci U S A. 2005 Mar 1;102(9):3284-9
Authors: Havlin RH, Tycko R
We demonstrate an experimental approach to structural studies of unfolded and partially folded proteins in which conformational distributions are probed at a site-specific level by 2D solid-state 13C NMR spectroscopy of glassy frozen solutions. Experiments on chemical...
nmrlearner
Journal club
0
11-24-2010 11:14 PM
[NMR paper] Site-specific backbone dynamics from a crystalline protein by solid-state NMR spectro
Site-specific backbone dynamics from a crystalline protein by solid-state NMR spectroscopy.
Related Articles Site-specific backbone dynamics from a crystalline protein by solid-state NMR spectroscopy.
J Am Chem Soc. 2004 Sep 22;126(37):11422-3
Authors: Giraud N, Böckmann A, Lesage A, Penin F, Blackledge M, Emsley L
Site-specific nitrogen-15 longitudinal relaxation rates are measured for the microcrystalline dimeric form of the protein Crh using multidimensional high-resolution solid-state NMR methods. The measured rates are used to provide a...
nmrlearner
Journal club
0
11-24-2010 10:01 PM
[NMR paper] Heparin binding to platelet factor-4. An NMR and site-directed mutagenesis study: arg
Heparin binding to platelet factor-4. An NMR and site-directed mutagenesis study: arginine residues are crucial for binding.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles Heparin binding to platelet factor-4. An NMR and site-directed mutagenesis study: arginine residues are crucial for binding.
Biochem J. 1995 Dec 1;312 ( Pt 2):357-65
Authors: Mayo KH, Ilyina E, Roongta V, Dundas M, Joseph J, Lai CK, Maione T, Daly TJ
Native platelet factor-4 (PF4) is an...
Site-specific identification of an a? fibril-heparin interaction site by using solid-state NMR spectroscopy.
Linear Mode
