Guanine nucleotide exchange factors (GEFs) of small GTPase (sGTPase) coordinate signal networks in normal cells and dysfunction in cancer. Therefore, effective monitoring of GEF activity is very important for studying the regulation of sGTPase signal transduction. In this study, we developed a 1D ^(19)F NMR-based method for rapid detection of the GEF activity of sGTPases. The activity of Arf6GEF in vitro and cell lysate environment can be conveniently detected by tracking the conformational...
[NMR paper] P-B desulfurization: an enabling method for protein chemical synthesis and site-specific deuteration
P-B desulfurization: an enabling method for protein chemical synthesis and site-specific deuteration
Native chemical ligation has provided a powerful tool for protein chemical synthesis. Herein, we report an unprecedented mild system (TCEP-NaBH4 or TCEP-LiBEt3H) for chemoselective peptide desulfurization to achieve effective protein synthesis via the native chemical ligation-desulfurization approach. This method, termed P-B desulfurization, features usage of common reagents, simplicity of operation, robustness, high yielding, clean conversion and versatile functionality compatibility...
nmrlearner
Journal club
0
10-03-2017 03:24 AM
[NMR paper] Erratum to: Protein-protein interaction analysis in crude bacterial lysates using combinational method of (19)F site-specific incorporation and (19)F NMR.
Erratum to: Protein-protein interaction analysis in crude bacterial lysates using combinational method of (19)F site-specific incorporation and (19)F NMR.
Related Articles Erratum to: Protein-protein interaction analysis in crude bacterial lysates using combinational method of (19)F site-specific incorporation and (19)F NMR.
Protein Cell. 2017 Mar 10;:
Authors: Li D, Zhang Y, He Y, Zhang C, Wang J, Xiong Y, Zhang L, Liu Y, Shi P, Tian C
PMID: 28284007
nmrlearner
Journal club
0
03-12-2017 12:32 PM
[NMR paper] Application of Site-Specific Spin Labeling for NMR Detecting Inhibitor-Induced Conformational Change of HIV-1 Reverse Transcriptase.
Application of Site-Specific Spin Labeling for NMR Detecting Inhibitor-Induced Conformational Change of HIV-1 Reverse Transcriptase.
Related Articles Application of Site-Specific Spin Labeling for NMR Detecting Inhibitor-Induced Conformational Change of HIV-1 Reverse Transcriptase.
ChemMedChem. 2016 Jan 25;
Authors: Seetaha S, Yagi-Utsumi M, Yamaguchi T, Ishii K, Hannongbua S, Choowongkomon K, Kato K
Abstract
Paramagnetism-assisted nuclear magnetic resonance (NMR) techniques can provide long-range structural information...
nmrlearner
Journal club
0
01-26-2016 03:40 PM
[NMR paper] A Magic-Angle Spinning NMR Method for the Site-Specific Measurement of Proton Chemical-Shift Anisotropy in Biological and Organic Solids.
A Magic-Angle Spinning NMR Method for the Site-Specific Measurement of Proton Chemical-Shift Anisotropy in Biological and Organic Solids.
Related Articles A Magic-Angle Spinning NMR Method for the Site-Specific Measurement of Proton Chemical-Shift Anisotropy in Biological and Organic Solids.
Isr J Chem. 2014 Feb 1;54(1-2):171-183
Authors: Hou G, Gupta R, Polenova T, Vega AJ
Abstract
Proton chemical shifts are a rich probe of structure and hydrogen bonding environments in organic and biological molecules. Until recently,...
nmrlearner
Journal club
0
12-09-2014 01:13 PM
A method for the second-site screening of K-Ras in the presence of a covalently attached first-site ligand
A method for the second-site screening of K-Ras in the presence of a covalently attached first-site ligand
Abstract
K-Ras is a well-validated cancer target but is considered to be â??undruggableâ?? due to the lack of suitable binding pockets. We previously discovered small molecules that bind weakly to K-Ras but wanted to improve their binding affinities by identifying ligands that bind near our initial hits that we could link together. Here we describe an approach for identifying second site ligands that uses a cysteine residue to covalently attach...
nmrlearner
Journal club
0
08-02-2014 12:38 PM
[NMR paper] Heteronuclear NMR As a 4-in-1 Analytical Platform for Detecting Modification-Specific Signatures of Therapeutic Insulin Formulations.
Heteronuclear NMR As a 4-in-1 Analytical Platform for Detecting Modification-Specific Signatures of Therapeutic Insulin Formulations.
Related Articles Heteronuclear NMR As a 4-in-1 Analytical Platform for Detecting Modification-Specific Signatures of Therapeutic Insulin Formulations.
Anal Chem. 2014 Feb 5;
Authors: Jin X, Kang S, Kwon H, Park S
Abstract
Detecting possible modifications of therapeutic proteins is a critical element of the quality control of protein drugs. Typically, a number of techniques are used to evaluate different...
nmrlearner
Journal club
0
02-07-2014 03:11 PM
[NMR paper] Site-specific identification of an a? fibril-heparin interaction site by using solid-state NMR spectroscopy.
Site-specific identification of an a? fibril-heparin interaction site by using solid-state NMR spectroscopy.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-2250-98-WileyOnlineLibrary-Button_120x27px_FullText.gif Related Articles Site-specific identification of an a? fibril-heparin interaction site by using solid-state NMR spectroscopy.
Angew Chem Int Ed Engl. 2012 Dec 21;51(52):13140-3
Authors: Madine J, Pandya MJ, Hicks MR, Rodger A, Yates EA, Radford SE, Middleton DA
Abstract
At the...
nmrlearner
Journal club
0
06-12-2013 11:42 AM
A Selective NMR Method for Detecting Choline Containing Compounds in Liver Tissue: Th
A Selective NMR Method for Detecting Choline Containing Compounds in Liver Tissue: The 1H-14N HSQC Experiment
Jiezhen Mao, Ling Jiang, Bin Jiang, Maili Liu and Xi-an Mao
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja107745g/aop/images/medium/ja-2010-07745g_0003.gif
Journal of the American Chemical Society
DOI: 10.1021/ja107745g
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/3IJa7rhbOjw
Site-Specific 19F NMR Method for Detecting Arf6 GEF Activity
Linear Mode
