Simultaneous Cross Polarization to 13C and 15N with 1H Detection at 60 kHz MAS Solid-state NMR

		BioNMR > Educational resources > Journal club
Simultaneous Cross Polarization to 13C and 15N with 1H Detection at 60 kHz MAS Solid-state NMR

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

12-12-2015, 05:40 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,732

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Simultaneous Cross Polarization to 13C and 15N with 1H Detection at 60 kHz MAS Solid-state NMR

Simultaneous Cross Polarization to 13C and 15N with 1H Detection at 60 kHz MAS Solid-state NMR

Publication date: Available online 12 December 2015
Source:Journal of Magnetic Resonance

Author(s): Bibhuti B. Das, Stanley J. Opella

We describe high resolution MAS solid-state NMR experiments that utilize 1H detection with 60 kHz magic angle spinning; simultaneous cross-polarization from 1H to 15N and 13C nuclei; bidirectional cross-polarization between 13C and 15N nuclei; detection of both amide nitrogen and aliphatic carbon 1H; and measurement of both 13C and 15N chemical shifts through multi-dimensional correlation experiments. Three-dimensional experiments correlate amide 1H and alpha 1H selectively with 13C or 15N nuclei in a polypeptide chain. Two separate three-dimensional spectra correlating 1H?/13C?/1HN and 1HN/15N/1H? are recorded simultaneously in a single experiment, demonstrating that a two-fold savings in experimental time is potentially achievable. Spectral editing using bidirectional coherence transfer pathways enables simultaneous magnetization transfers between 15N, 13C?(i) and 13C’(i-1), facilitating intra- and inter- residue correlations for sequential resonance assignment. Non-uniform sampling is integrated into the experiments, further reducing the length of experimental time.
Graphical abstract

Source: Journal of Magnetic Resonance

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] Optimization of cross-polarization at low radiofrequency fields for sensitivity enhancement in solid-state NMR of membrane proteins reconstituted in magnetically aligned bicelles. Optimization of cross-polarization at low radiofrequency fields for sensitivity enhancement in solid-state NMR of membrane proteins reconstituted in magnetically aligned bicelles. Optimization of cross-polarization at low radiofrequency fields for sensitivity enhancement in solid-state NMR of membrane proteins reconstituted in magnetically aligned bicelles. J Magn Reson. 2015 Apr 28;256:14-22 Authors: Koroloff SN, Nevzorov AA Abstract Solid-state NMR (ssNMR) of oriented membrane proteins (MPs) is capable of providing...	nmrlearner	Journal club	0	05-13-2015 02:01 PM
[NMR paper] Optimization of cross-polarization at low radiofrequency fields for sensitivity enhancement in solid-state NMR of membrane proteins reconstituted in magnetically aligned bicelles Optimization of cross-polarization at low radiofrequency fields for sensitivity enhancement in solid-state NMR of membrane proteins reconstituted in magnetically aligned bicelles Publication date: Available online 28 April 2015 Source:Journal of Magnetic Resonance</br> Author(s): Sophie N. Koroloff , Alexander A. Nevzorov</br> Solid-state NMR (ssNMR) of oriented membrane proteins (MPs) is capable of providing structural and dynamic information at nearly physiological conditions. However, NMR experiments performed on oriented membrane proteins generally suffer from...	nmrlearner	Journal club	0	04-28-2015 12:40 PM
[NMR paper] Asymmetric simultaneous phase-inversion cross-polarization in solid-state MAS NMR: Relaxing selective polarization transfer condition between two dilute spins. Asymmetric simultaneous phase-inversion cross-polarization in solid-state MAS NMR: Relaxing selective polarization transfer condition between two dilute spins. Related Articles Asymmetric simultaneous phase-inversion cross-polarization in solid-state MAS NMR: Relaxing selective polarization transfer condition between two dilute spins. J Magn Reson. 2014 Mar 16;242C:214-219 Authors: Zhang Z, Fu R, Li J, Yang J Abstract Double cross polarization (DCP) has been widely used for heteronuclear polarization transfer between (13)C...	nmrlearner	Journal club	0	04-03-2014 12:59 PM
Asymmetric simultaneous phase-inversion cross-polarization in solid-state MAS NMR: relaxing selective polarization transfer condition between two dilute spins Asymmetric simultaneous phase-inversion cross-polarization in solid-state MAS NMR: relaxing selective polarization transfer condition between two dilute spins Publication date: Available online 16 March 2014 Source:Journal of Magnetic Resonance</br> Author(s): Zhengfeng Zhang , Riqiang Fu , Jianping Li , Jun Yang</br> Double cross polarization (DCP) has been widely used for heteronuclear polarization transfer between 13C and 15N in solid-state magic-angle spinning (MAS) NMR. However, DCP is such sensitive to experimental settings that small variations or...	nmrlearner	Journal club	0	03-16-2014 04:09 PM
Theoretical Aspects of Dynamic Nuclear Polarization in the Solid State: The Influence of High Radical Concentrations on the Solid Effect and Cross Effect Mechanisms From The DNP-NMR Blog: Theoretical Aspects of Dynamic Nuclear Polarization in the Solid State: The Influence of High Radical Concentrations on the Solid Effect and Cross Effect Mechanisms Hovav, Y., et al., Theoretical Aspects of Dynamic Nuclear Polarization in the Solid State: The Influence of High Radical Concentrations on the Solid Effect and Cross Effect Mechanisms. Appl. Magn. Reson., 2012. 43(1-2): p. 21-41. http://dx.doi.org/10.1007/s00723-012-0359-0	nmrlearner	News from NMR blogs	0	11-21-2013 01:14 AM
Dual-band Selective Double Cross Polarization for Heteronuclear Polarization Transfer between Dilute Spins in Solid-State MAS NMR Dual-band Selective Double Cross Polarization for Heteronuclear Polarization Transfer between Dilute Spins in Solid-State MAS NMR Publication year: 2012 Source:Journal of Magnetic Resonance</br> Zhengfeng Zhang, Yimin Miao, Xiaoli Liu, Jun Yang, Conggang Li, Feng Deng, Riqiang Fu</br> A sinusoidal modulation scheme is described for selective heteronuclear polarization transfer between two dilute spins in double cross polarization magic-angle-spinning nuclear magnetic resonance spectroscopy. During the second N->C cross polarization, the 13C RF amplitude is...	nmrlearner	Journal club	0	03-09-2012 09:16 AM
Dual-band Selective Double Cross Polarization for Heteronuclear Polarization Transfer between Dilute Spins in Solid-State MAS NMR Dual-band Selective Double Cross Polarization for Heteronuclear Polarization Transfer between Dilute Spins in Solid-State MAS NMR Publication year: 2012 Source: Journal of Magnetic Resonance, Available online 5 March 2012</br> ZhengfengZhang, YiminMiao, XiaoliLiu, JunYang, Conggang*Li, ...</br> A sinusoidal modulation scheme is described for selective heteronuclear polarization transfer between two dilute spins in double cross polarization magic-angle-spinning nuclear magnetic resonance spectroscopy. During the second N->C cross polarization, theC RF amplitude is modulated...	nmrlearner	Journal club	0	03-06-2012 06:04 AM
Rapid Solid-State NMR of Deuterated Proteins by Interleaved Cross-Polarization fromH andH Nuclei Rapid Solid-State NMR of Deuterated Proteins by Interleaved Cross-Polarization fromH andH Nuclei Publication year: 2011 Source: Journal of Magnetic Resonance, Available online 9 November 2011</br> MortenBjerring, BeritPaaske, HartmutOschkinat, UmitAkbey, Niels Chr.*Nielsen</br> We present a novel sampling strategy, interleaving acquisition of multiple NMR spectra by exploiting initial polarization subsequently fromH andH spins, taking advantage of their differentT1relaxation times. DifferentH- andH-polarization based spectra are in this way simultaneously recorded improving either...	nmrlearner	Journal club	0	11-10-2011 07:38 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off