Abstract Thermal convection and high intensity solvent resonances can significantly hamper diffusion estimates in pulsed gradient spin-echo nuclear magnetic resonance diffusion experiments on biomolecule samples. To overcome these two problems, a new double functional NMR diffusion sequence, double echo PGSTE-WATERGATE, is presented. The new sequence provides excellent convection compensation and solvent suppression (with a suppression factor in excess of at least 105 in a single scan) in biomolecular NMR diffusion experiments. Due to its stimulated echo nature, the new sequence is much less susceptible to spinā??spin relaxation than Hahn spin-echo based sequences. Furthermore, the new sequence is not susceptible to spin diffusion due to the application of bipolar pulsed gradients. The new sequence is also much easier to set up compared to previously developed stimulated echo based convection compensation and solvent suppression sequence. The utility of the new sequence is demonstrated on an aqueous lysozyme sample.
Content Type Journal Article
Pages 295-299
DOI 10.1007/s10858-009-9367-2
Authors
Gang Zheng, University of Western Sydney Nanoscale Organisation and Dynamics Group, College of Health and Science Penrith South DC NSW 1797 Australia
William S. Price, University of Western Sydney Nanoscale Organisation and Dynamics Group, College of Health and Science Penrith South DC NSW 1797 Australia
Li Ion Diffusion in the Anode Material Li12Si7: Ultrafast Quasi-1D Diffusion and Two Distinct Fast 3D Jump Processes Separately Revealed by 7Li NMR Relaxometry
Li Ion Diffusion in the Anode Material Li12Si7: Ultrafast Quasi-1D Diffusion and Two Distinct Fast 3D Jump Processes Separately Revealed by 7Li NMR Relaxometry
Alexander Kuhn, Puravankara Sreeraj, Rainer Po?ttgen, Hans-Dieter Wiemho?fer, Martin Wilkening and Paul Heitjans
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja2020108/aop/images/medium/ja-2011-020108_0005.gif
Journal of the American Chemical Society
DOI: 10.1021/ja2020108
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA...
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[NMR paper] Simultaneous assignment and structure determination of protein backbones by using NMR
Simultaneous assignment and structure determination of protein backbones by using NMR dipolar couplings.
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Angew Chem Int Ed Engl. 2004 Jun 28;43(26):3479-81
Authors: Jung YS, Sharma M, Zweckstetter M
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[NMR paper] Simultaneous assignment and structure determination of a membrane protein from NMR or
Simultaneous assignment and structure determination of a membrane protein from NMR orientational restraints.
Simultaneous assignment and structure determination of a membrane protein from NMR orientational restraints.
Protein Sci. 2003 Mar;12(3):403-11
Authors: Marassi FM, Opella SJ
A solid-state NMR approach for simultaneous resonance assignment and three-dimensional structure determination of a membrane protein in lipid bilayers is described. The approach is based on the scattering, hence the descriptor "shotgun," of (15)N-labeled amino...
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[NMR paper] Residue-specific real-time NMR diffusion experiments define the association states of
Residue-specific real-time NMR diffusion experiments define the association states of proteins during folding.
Related Articles Residue-specific real-time NMR diffusion experiments define the association states of proteins during folding.
J Am Chem Soc. 2002 Jun 19;124(24):7156-62
Authors: Buevich AV, Baum J
Characterizing the association states of proteins during folding is critical for understanding the nature of protein-folding intermediates and protein-folding pathways, protein aggregation, and disease-related aggregation. To study the...
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11-24-2010 08:49 PM
[NMR paper] Convection compensated electrophoretic NMR.
Convection compensated electrophoretic NMR.
Related Articles Convection compensated electrophoretic NMR.
J Magn Reson. 2001 Jun;150(2):126-31
Authors: He Q, Wei Z
A novel method of convection compensated ENMR (CC-ENMR) has been developed to detect electrophoretic motion of ionic species in the presence of bulk solution convection. This was accomplished using a gradient moment nulling technique to remove spectral artifacts from heat-induced convection and using the polarity switch of the applied electric field to retain spin phase modulations...
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Solvent signal suppression in NMR
Solvent signal suppression in NMR
Publication year: 2010
Source: Progress in Nuclear Magnetic Resonance Spectroscopy, In Press, Accepted Manuscript, Available online 25 January 2010</br>
Gang, Zheng , William S., Price</br>
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08-16-2010 03:50 AM
Nitrogen-detected CAN and CON experiments as alternative experiments for main chain N
Abstract Heteronuclear direct-detection experiments, which utilize the slower relaxation properties of low Ī³ nuclei, such as 13C have recently been proposed for sequence-specific assignment and structural analyses of large, unstructured, and/or paramagnetic proteins. Here we present two novel 15N direct-detection experiments. The CAN experiment sequentially connects amide 15N resonances using 13CĪ± chemical shift matching, and the CON experiment connects the preceding 13Cā?² nuclei. When starting from the same carbon polarization, the intensities of nitrogen signals detected in the CAN or...
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Spatially encoded strategies in the execution of biomolecular-oriented 3D NMR experiments
Spatially encoded strategies in the execution of biomolecular-oriented 3D NMR experiments
Mor Mishkovsky, Maayan Gal and Lucio Frydman
Journal of Biomolecular NMR; 2007; 39(4); pp 291-301
Abstract:
Three-dimensional nuclear magnetic resonance (3D NMR) provides one of the foremost analytical tools available for the elucidation of biomolecular structure, function and dynamics. Executing a 3D NMR experiment generally involves scanning a series of time-domain signals S(t 3), as a function of two time variables (t 1, t 2) which need to undergo parametric incrementations throughout...
Simultaneous convection compensation and solvent suppression in biomolecular NMR diffusion experiments
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