NMR paper A simple and inexpensive preparation of perdeuterated sorbitol for use as a biomacrom

		BioNMR > Educational resources > Journal club
[NMR paper] A simple and inexpensive preparation of perdeuterated sorbitol for use as a biomacrom

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

11-18-2010, 09:15 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,734

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

A simple and inexpensive preparation of perdeuterated sorbitol for use as a biomacrom

A simple and inexpensive preparation of perdeuterated sorbitol for use as a biomacromolecule stabilization agent in NMR studies.

Related Articles A simple and inexpensive preparation of perdeuterated sorbitol for use as a biomacromolecule stabilization agent in NMR studies.

J Biomol NMR. 2000 Apr;16(4):339-42

Authors: Horita DA, Farnsworth DW, Byrd RA

Sorbitol is an excellent protein-stabilization agent, but it is typically used at high concentrations where the 1H signals can interfere with NMR data collection and analysis. Deuteration of sorbitol can ameliorate this problem; however, perdeuterated sorbitol is not commercially available. We describe a simple and inexpensive method for preparation of perdeuterated sorbitol from perdeuterated glucose. The method is described explicitly and examples are given where the use of perdeuterated sorbitol has allowed the extraction of information, from NMR spectra, that is otherwise unobtainable.

PMID: 10826885 [PubMed - indexed for MEDLINE]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[Question from NMRWiki Q&A forum] RDC gel preparation RDC gel preparation I have seen many different protocols on making acrylamide gels for RDC measurements which include usually one of the following 1)drying the gel and re-swelling with protein solution2)allowing equilibration of protein into an already swelled gel It seems as though you can get a much higher protein conc. if you dry the gel so what is the advantage to letting it equilibrate? Thanks	nmrlearner	News from other NMR forums	0	01-24-2012 07:54 PM
Homonuclear Mixing Sequences for Perdeuterated Proteins Homonuclear Mixing Sequences for Perdeuterated Proteins Publication year: 2010 Source: Journal of Magnetic Resonance, In Press, Accepted Manuscript, Available online 26 October 2010</br> Kuo-Ying, Huang , Ansgar B., Siemer , Ann E., McDermott</br> We test the performance of several 13C homonuclear mixing sequences on perdeuterated microcrystalline ubiquitin. All sequences were applied without 1H decoupling and at relatively low MAS frequencies. We found that RFDR gave the highest overall transfer efficiency and that DREAM performs surprisingly well under these conditions being twice...	nmrlearner	Journal club	0	10-27-2010 08:51 AM
[NMR paper] Anisotropic rotational diffusion of perdeuterated HIV protease from 15N NMR relaxatio Anisotropic rotational diffusion of perdeuterated HIV protease from 15N NMR relaxation measurements at two magnetic fields. Related Articles Anisotropic rotational diffusion of perdeuterated HIV protease from 15N NMR relaxation measurements at two magnetic fields. J Biomol NMR. 1996 Oct;8(3):273-84 Authors: Tjandra N, Wingfield P, Stahl S, Bax A 15N NMR relaxation times in perdeuterated HIV-1 protease, complexed with the sub-nanomolar inhibitor DMP323, have been measured at 600 and 360 MHz 1H frequency. The relative magnitudes of the principal...	nmrlearner	Journal club	0	08-22-2010 02:20 PM
[U. of Ottawa NMR Facility Blog] Free and Inexpensive NMR Processing Software for Students Free and Inexpensive NMR Processing Software for Students Processing NMR data has just become more affordable for students. Our friends at Advanced Chemistry Development have recently decided to make their NMR processing software free of charge to academics. As a student, you can put this software on your personal laptop or PC and process your NMR data at home or anywhere your travels take you. You can register and download the software here. They have even started a BLOG dealing with use of the software. I think I speak for all students when I say "Thanks guys!!" Also, our friends at...	nmrlearner	News from NMR blogs	0	08-21-2010 08:15 PM
NMR Sample Preparation from NESG Wiki NMR Sample Preparation from NMR2.0 Wiki http://www.nmr2.buffalo.edu/nesg.wiki/NMR_Sample_Preparation originally posted by Main.Gaohua.Liu (11 Feb 2007) <!-- start content --> <table style="width: 76px; height: 63px;" id="toc" class="toc" summary="Contents"><tbody><tr><td>Contents	markber	BioNMR Wiki	0	08-16-2010 04:04 AM
Sample preparation Sample preparation Samples The Samples program is designed to collect and store some essential information pertaining to the samples used to record the spectra. Residual Dipolar Couplings Bicelle preparation notes from Avanti Instructions for bicelle preparation from Avanti	ale51	BioNMR Wiki	0	01-29-2005 01:35 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off