NMR paper A Simple Fluorescence Affinity Assay to Decipher Uranyl-Binding to Native Proteins

		BioNMR > Educational resources > Journal club
[NMR paper] A Simple Fluorescence Affinity Assay to Decipher Uranyl-Binding to Native Proteins

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

04-25-2022, 09:37 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

A Simple Fluorescence Affinity Assay to Decipher Uranyl-Binding to Native Proteins

A Simple Fluorescence Affinity Assay to Decipher Uranyl-Binding to Native Proteins

Angewandte Chemie International Edition, Accepted Article.

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] Ultrasensitive Detection of Aqueous Uranyl Based on Uranyl-Triggered Protein Photocleavage Ultrasensitive Detection of Aqueous Uranyl Based on Uranyl-Triggered Protein Photocleavage Angewandte Chemie International Edition, Accepted Article. More...	nmrlearner	Journal club	0	01-04-2022 08:36 PM
Quantification of Membrane Protein Self-Associationwith a High-Throughput Compatible Fluorescence Assay Quantification of Membrane Protein Self-Associationwith a High-Throughput Compatible Fluorescence Assay http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.7b00009/20170403/images/medium/bi-2017-000095_0005.gif Biochemistry DOI: 10.1021/acs.biochem.7b00009 http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/bichaw/~4/U1NmCao9Q78 More...	nmrlearner	Journal club	0	04-04-2017 09:40 AM
Native Mass Spectrometry Analysis of OligomerizationStates of Fluorescence Recovery Protein and Orange Carotenoid Protein:Two Proteins Involved in the Cyanobacterial Photoprotection Cycle Native Mass Spectrometry Analysis of OligomerizationStates of Fluorescence Recovery Protein and Orange Carotenoid Protein:Two Proteins Involved in the Cyanobacterial Photoprotection Cycle http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.6b01094/20161220/images/medium/bi-2016-01094s_0008.gif Biochemistry DOI: 10.1021/acs.biochem.6b01094 http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/bichaw/~4/l5AEFIR2Wkc More...	nmrlearner	Journal club	0	12-21-2016 07:20 AM
[NMR paper] Identification of Small-Molecule Inhibitors of the HuR/RNA Interaction Using a Fluorescence Polarization Screening Assay Followed by NMR Validation. Identification of Small-Molecule Inhibitors of the HuR/RNA Interaction Using a Fluorescence Polarization Screening Assay Followed by NMR Validation. Related Articles Identification of Small-Molecule Inhibitors of the HuR/RNA Interaction Using a Fluorescence Polarization Screening Assay Followed by NMR Validation. PLoS One. 2015;10(9):e0138780 Authors: Wang Z, Bhattacharya A, Ivanov DN Abstract The human antigen R (HuR) stabilizes many mRNAs of proto-oncogene, transcription factors, cytokines and growth factors by recognizing...	nmrlearner	Journal club	0	09-22-2015 06:40 PM
[NMR paper] Gradual Disordering of the Native State on a Slow Two-State Folding Protein Monitored by Single-Molecule Fluorescence Spectroscopy and NMR. Gradual Disordering of the Native State on a Slow Two-State Folding Protein Monitored by Single-Molecule Fluorescence Spectroscopy and NMR. Gradual Disordering of the Native State on a Slow Two-State Folding Protein Monitored by Single-Molecule Fluorescence Spectroscopy and NMR. J Phys Chem B. 2013 Jun 24; Authors: Campos LA, Sadqi M, Liu J, Wang X, English DS, Munoz V Abstract Theory predicts that folding free energy landscapes are intrinsically malleable, and as such are expected to respond to perturbations in topographically complex...	nmrlearner	Journal club	0	06-27-2013 01:52 AM
An ensemble dynamics approach to decipher solid-state NMR observables of membrane proteins An ensemble dynamics approach to decipher solid-state NMR observables of membrane proteins February 2012 Publication year: 2012 Source:Biochimica et Biophysica Acta (BBA) - Biomembranes, Volume 1818, Issue 2</br> </br> Solid-state NMR (SSNMR) is an invaluable tool for determining orientations of membrane proteins and peptides in lipid bilayers. Such orientational descriptions provide essential information about membrane protein functions. However, when a semi-static single conformer model is used to interpret various SSNMR observables, important dynamics information can...	nmrlearner	Journal club	0	02-03-2013 10:13 AM
NMR and Fluorescence Studiesof Drug Binding to theFirst Nucleotide Binding Domain of SUR2A NMR and Fluorescence Studiesof Drug Binding to theFirst Nucleotide Binding Domain of SUR2A http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/bi301019e/aop/images/medium/bi-2012-01019e_0008.gif Biochemistry DOI: 10.1021/bi301019e http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/bichaw/~4/VpjFg9eH8kQ More...	nmrlearner	Journal club	0	11-01-2012 10:38 PM
An ensemble dynamics approach to decipher solid-sate NMR observables of membrane proteins. An ensemble dynamics approach to decipher solid-sate NMR observables of membrane proteins. An ensemble dynamics approach to decipher solid-sate NMR observables of membrane proteins. Biochim Biophys Acta. 2011 Aug 8; Authors: Im W, Jo S, Kim T Abstract Solid-state NMR (SSNMR) is an invaluable tool for determining orientations of membrane proteins and peptides in lipid bilayers. Such orientational descriptions provide essential information about membrane protein functions. However, when a semi-static single conformer model is used to...	nmrlearner	Journal club	1	08-25-2011 07:04 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off