Publication date: Available online 14 December 2013 Source:Journal of Magnetic Resonance
Author(s): Christoph Wiedemann , Peter Bellstedt , Anika Kirschstein , Sabine Häfner , Christian Herbst , Matthias Görlach , Ramadurai Ramachandran
Two different NMR pulse schemes involving sequential 1H data acquisition are presented for achieving protein backbone sequential resonance assignments: (i) acquisition of 3D {HCCNH & HNCACONH} and (ii) collection of 3D {HNCOCANH & HNCACONH} chemical shift correlation spectra using uniformly 13C,15N labelled proteins. The sequential acquisition of these spectra reduces the overall experimental time by a factor of ? 2 as compared to individual acquisitions. The suitability of this approach is experimentally demonstrated for the C-terminal winged helix (WH) domain of the minichromosome maintenance (MCM) complex of Sulfolobus solfataricus. Graphical abstract
[NMR paper] Solid-state NMR sequential assignments of the amyloid core of full-length Sup35p.
Solid-state NMR sequential assignments of the amyloid core of full-length Sup35p.
Solid-state NMR sequential assignments of the amyloid core of full-length Sup35p.
Biomol NMR Assign. 2013 Aug 14;
Authors: Schütz AK, Habenstein B, Luckgei N, Bousset L, Sourigues Y, Nielsen AB, Melki R, Böckmann A, Meier BH
Abstract
Sup35p is a yeast prion and is responsible for the trait in Saccharomyces cerevisiae. With 685 amino acids, full-length soluble and fibrillar Sup35p are challenging targets for structural biology as they cannot be investigated...
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08-15-2013 07:45 PM
[NMR paper] Solid-state NMR sequential assignments of the amyloid core of Sup35pNM.
Solid-state NMR sequential assignments of the amyloid core of Sup35pNM.
Solid-state NMR sequential assignments of the amyloid core of Sup35pNM.
Biomol NMR Assign. 2013 Aug 10;
Authors: Luckgei N, Schütz AK, Habenstein B, Bousset L, Sourigues Y, Melki R, Meier BH, Böckmann A
Abstract
Sup35pNM represents the N-terminal and middle (M) domains of the yeast Saccharomyces cerevisiae prion Sup35p. This fragment is commonly used for structural and functional studies of Sup35p. We here present a solid-state NMR study of fibrils formed by this...
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08-13-2013 04:26 PM
Simultaneous acquisition of 13Cαâ??15N and 1Hâ??15Nâ??15N sequential correlations in proteins: application of dual receivers in 3D HNN
Simultaneous acquisition of 13Cαâ??15N and 1Hâ??15Nâ??15N sequential correlations in proteins: application of dual receivers in 3D HNN
Abstract We describe here, adaptation of the HNN pulse sequence for multiple nuclei detection using two independent receivers by utilizing the detectable 13Cα transverse magnetization which was otherwise dephased out in the conventional HNN experiment. It enables acquisition of 2D 13Cαâ??15N sequential correlations along with the standard 3D 15Nâ??15Nâ??1H correlations, which provides directionality to sequential walk in HNN, on one hand, and enhances...
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12-31-2011 10:40 AM
Solid-state NMR sequential assignments of ?-synuclein.
Solid-state NMR sequential assignments of ?-synuclein.
Solid-state NMR sequential assignments of ?-synuclein.
Biomol NMR Assign. 2011 Jul 9;
Authors: Gath J, Habenstein B, Bousset L, Melki R, Meier BH, Böckmann A
Parkinson's disease is amongst the most frequent and most devastating neurodegenerative diseases. It is tightly associated with the assembly of proteins into high-molecular weight protein species, which propagate between neurons in the central nervous system. The principal protein involved in this process is ?-synuclein which is a...
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07-12-2011 06:23 PM
[NMR paper] Solid state NMR sequential resonance assignments and conformational analysis of the 2
Solid state NMR sequential resonance assignments and conformational analysis of the 2x10.4 kDa dimeric form of the Bacillus subtilis protein Crh.
Related Articles Solid state NMR sequential resonance assignments and conformational analysis of the 2x10.4 kDa dimeric form of the Bacillus subtilis protein Crh.
J Biomol NMR. 2003 Dec;27(4):323-39
Authors: Böckmann A, Lange A, Galinier A, Luca S, Giraud N, Juy M, Heise H, Montserret R, Penin F, Baldus M
Solid state NMR sample preparation and resonance assignments of the U- 2x10.4 kDa dimeric form...
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11-24-2010 09:16 PM
[NMR paper] Sequential 1H, 13C, and 15N NMR assignments and solution conformation of apokedarcidi
Sequential 1H, 13C, and 15N NMR assignments and solution conformation of apokedarcidin.
Related Articles Sequential 1H, 13C, and 15N NMR assignments and solution conformation of apokedarcidin.
Biochemistry. 1994 Sep 27;33(38):11438-52
Authors: Constantine KL, Colson KL, Wittekind M, Friedrichs MS, Zein N, Tuttle J, Langley DR, Leet JE, Schroeder DR, Lam KS
Kedarcidin is a recently discovered antitumor antibiotic chromoprotein. The solution conformation of the kedarcidin apoprotein (114 residues) has been characterized by heteronuclear...
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08-22-2010 03:29 AM
[NMR paper] Dihydrofolate reductase: sequential resonance assignments using 2D and 3D NMR and sec
Dihydrofolate reductase: sequential resonance assignments using 2D and 3D NMR and secondary structure determination in solution.
Related Articles Dihydrofolate reductase: sequential resonance assignments using 2D and 3D NMR and secondary structure determination in solution.
Biochemistry. 1991 Jun 25;30(25):6330-41
Authors: Carr MD, Birdsall B, Frenkiel TA, Bauer CJ, Jimenez-Barbero J, Polshakov VI, McCormick JE, Roberts GC, Feeney J
Three-dimensional (3D) heteronuclear NMR techniques have been used to make sequential 1H and 15N resonance...
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08-21-2010 11:16 PM
[NMR paper] 1H NMR studies of echistatin in solution. Sequential resonance assignments and second
1H NMR studies of echistatin in solution. Sequential resonance assignments and secondary structure.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif Related Articles 1H NMR studies of echistatin in solution. Sequential resonance assignments and secondary structure.
Eur J Biochem. 1991 Dec 5;202(2):315-21
Authors: Dalvit C, Widmer H, Bovermann G, Breckenridge R, Metternich R
Two-dimensional 1H-NMR methods have been used to obtain complete proton...
Sequential protein NMR assignments in the liquid state via sequential data acquisition
Linear Mode
