NMR paper A sequential HNCA NMR pulse sequence for protein backbone assignment.

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[NMR paper] A sequential HNCA NMR pulse sequence for protein backbone assignment.

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NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

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Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

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HADDOCK

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V-NMR

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Backbone S2

Methyl S2

B-factor

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Gromacs

Amber

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Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

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Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

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Protein solubility:

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11-19-2010, 08:32 PM

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A sequential HNCA NMR pulse sequence for protein backbone assignment.

A sequential HNCA NMR pulse sequence for protein backbone assignment.

Related Articles A sequential HNCA NMR pulse sequence for protein backbone assignment.

J Magn Reson. 2001 May;150(1):100-4

Authors: Meissner A, Sørensen OW

The conventional HNCA pulse sequence suffers from the ambiguity that it cannot distinguish inter- and intraresidue correlations because the one-bond and two-bond J(NC(alpha)) coupling constants are of similar magnitude. This paper presents a novel pulse sequence, sequential HNCA, that leads to a spectrum exhibiting exclusively interresidue correlations. This important sequential information has so far usually been obtained by an HN(CO)CA experiment that for medium field strengths typically also is more sensitive than HNCA. However, for increasing static magnetic fields the chemical shift anisotropy relaxation mechanism of carbonyl carbons becomes more and more efficient, leading to a degradation of the HN(CO)CA sensitivity. Hence there is a point where the sequential HNCA experiment becomes the most sensitive option for sequential N-C(alpha) correlation.

PMID: 11330987 [PubMed]

Source: PubMed

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