NMR paper Sequential 1H NMR assignments of kistrin, a potent platelet aggregation inhibitor and

		BioNMR > Educational resources > Journal club
[NMR paper] Sequential 1H NMR assignments of kistrin, a potent platelet aggregation inhibitor and

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

08-21-2010, 11:41 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Sequential 1H NMR assignments of kistrin, a potent platelet aggregation inhibitor and

Sequential 1H NMR assignments of kistrin, a potent platelet aggregation inhibitor and glycoprotein IIb-IIIa antagonist.

Related Articles Sequential 1H NMR assignments of kistrin, a potent platelet aggregation inhibitor and glycoprotein IIb-IIIa antagonist.

Biochemistry. 1992 Feb 4;31(4):1031-9

Authors: Adler M, Wagner G

Sequence-specific nuclear magnetic resonances assignments have been obtained for the protons of kistrin. Kistrin is a small naturally occurring snake venom protein that inhibits platelet aggregation by blocking the interaction of fibrinogen with the membrane-bound glycoprotein IIb-IIIa (GP IIb-IIIa), a receptor from the integrin family. Kistrin has an Arg-Gly-Asp sequence which is believed to form an adhesion recognition sequence that is essential for activity. Therefore, the interaction between kistrin and GP IIb-IIIa may provide important information on the motif used by integrins to recognize their target proteins which bear RGD sequences. Kistrin consists of 68 residues and contains six intramolecular disulfide bonds. Although one-third of the amide protons are protected from exchange with the solvent, there appears to be little or no regular secondary structure. The large number of NOE's between residues separated by two and three positions in the sequence indicates that the protein contains a large number of tightly packed loops. Along with the sequential assignments, this paper also discusses the construction and use of computerized data bases for manipulating NMR results. A strategy for computer-assisted sequential resonance using these data bases is also presented.

PMID: 1734953 [PubMed - indexed for MEDLINE]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Solid-state NMR sequential assignments of ?-synuclein. Solid-state NMR sequential assignments of ?-synuclein. Solid-state NMR sequential assignments of ?-synuclein. Biomol NMR Assign. 2011 Jul 9; Authors: Gath J, Habenstein B, Bousset L, Melki R, Meier BH, Böckmann A Parkinson's disease is amongst the most frequent and most devastating neurodegenerative diseases. It is tightly associated with the assembly of proteins into high-molecular weight protein species, which propagate between neurons in the central nervous system. The principal protein involved in this process is ?-synuclein which is a...	nmrlearner	Journal club	0	07-12-2011 06:23 PM
[NMR paper] The design of a potent inhibitor of the hepatitis C virus NS3 protease: BILN 2061--fr The design of a potent inhibitor of the hepatitis C virus NS3 protease: BILN 2061--from the NMR tube to the clinic. Related Articles The design of a potent inhibitor of the hepatitis C virus NS3 protease: BILN 2061--from the NMR tube to the clinic. Biopolymers. 2004;76(4):309-23 Authors: Tsantrizos YS The virally encoded serine protease NS3/NS4A is essential to the life cycle of the hepatitis C virus (HCV), an important human pathogen causing chronic hepatitis, cirrhosis of the liver, and hepatocellular carcinoma. Until very recently, the...	nmrlearner	Journal club	0	11-24-2010 09:25 PM
Discovery of a potent and selective Bcl-2 inhibitor using SAR by NMR. Discovery of a potent and selective Bcl-2 inhibitor using SAR by NMR. Related Articles Discovery of a potent and selective Bcl-2 inhibitor using SAR by NMR. Bioorg Med Chem Lett. 2010 Sep 15; Authors: Petros AM, Huth JR, Oost T, Park CM, Ding H, Wang X, Zhang H, Nimmer P, Mendoza R, Sun C, Mack J, Walter K, Dorwin S, Gramling E, Ladror U, Rosenberg SH, Elmore SW, Fesik SW, Hajduk PJ The Bcl-2 family of proteins plays a major role in the regulation of apoptosis, or programmed cell death. Overexpression of the anti-apoptotic members of this...	nmrlearner	Journal club	0	09-29-2010 08:04 AM
[NMR paper] Sequential 1H, 13C, and 15N NMR assignments and solution conformation of apokedarcidi Sequential 1H, 13C, and 15N NMR assignments and solution conformation of apokedarcidin. Related Articles Sequential 1H, 13C, and 15N NMR assignments and solution conformation of apokedarcidin. Biochemistry. 1994 Sep 27;33(38):11438-52 Authors: Constantine KL, Colson KL, Wittekind M, Friedrichs MS, Zein N, Tuttle J, Langley DR, Leet JE, Schroeder DR, Lam KS Kedarcidin is a recently discovered antitumor antibiotic chromoprotein. The solution conformation of the kedarcidin apoprotein (114 residues) has been characterized by heteronuclear...	nmrlearner	Journal club	0	08-22-2010 03:29 AM
[NMR paper] Dihydrofolate reductase: sequential resonance assignments using 2D and 3D NMR and sec Dihydrofolate reductase: sequential resonance assignments using 2D and 3D NMR and secondary structure determination in solution. Related Articles Dihydrofolate reductase: sequential resonance assignments using 2D and 3D NMR and secondary structure determination in solution. Biochemistry. 1991 Jun 25;30(25):6330-41 Authors: Carr MD, Birdsall B, Frenkiel TA, Bauer CJ, Jimenez-Barbero J, Polshakov VI, McCormick JE, Roberts GC, Feeney J Three-dimensional (3D) heteronuclear NMR techniques have been used to make sequential 1H and 15N resonance...	nmrlearner	Journal club	0	08-21-2010 11:16 PM
[NMR paper] 1H-NMR studies of bovine platelet factor 4: histidine assignments and interactions wi 1H-NMR studies of bovine platelet factor 4: histidine assignments and interactions with heparin. Related Articles 1H-NMR studies of bovine platelet factor 4: histidine assignments and interactions with heparin. Biochim Biophys Acta. 1991 Jun 24;1078(2):208-18 Authors: Talpas CJ, Walz DA, Lee L 1H-NMR spectroscopy has been used to assign and to characterize the two histidine C2H resonances of the heparin binding protein, bovine platelet factor 4. One histidine has a pKa value of 6.51 at 27 degrees C; the second histidine exhibits 2 pKa values...	nmrlearner	Journal club	0	08-21-2010 11:16 PM
[NMR paper] 1H NMR studies of echistatin in solution. Sequential resonance assignments and second 1H NMR studies of echistatin in solution. Sequential resonance assignments and secondary structure. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif Related Articles 1H NMR studies of echistatin in solution. Sequential resonance assignments and secondary structure. Eur J Biochem. 1991 Dec 5;202(2):315-21 Authors: Dalvit C, Widmer H, Bovermann G, Breckenridge R, Metternich R Two-dimensional 1H-NMR methods have been used to obtain complete proton...	nmrlearner	Journal club	0	08-21-2010 11:12 PM
[NMR paper] Sequential 1H NMR assignments and secondary structure of the B domain of staphylococc Sequential 1H NMR assignments and secondary structure of the B domain of staphylococcal protein A: structural changes between the free B domain in solution and the Fc-bound B domain in crystal. Related Articles Sequential 1H NMR assignments and secondary structure of the B domain of staphylococcal protein A: structural changes between the free B domain in solution and the Fc-bound B domain in crystal. Biochemistry. 1990 Sep 18;29(37):8787-93 Authors: Torigoe H, Shimada I, Saito A, Sato M, Arata Y The recombinant B domain (FB) of staphylococcal...	nmrlearner	Journal club	0	08-21-2010 11:04 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off