Related ArticlesSequential 1H, 13C, and 15N NMR assignments and solution conformation of apokedarcidin.
Biochemistry. 1994 Sep 27;33(38):11438-52
Authors: Constantine KL, Colson KL, Wittekind M, Friedrichs MS, Zein N, Tuttle J, Langley DR, Leet JE, Schroeder DR, Lam KS
Kedarcidin is a recently discovered antitumor antibiotic chromoprotein. The solution conformation of the kedarcidin apoprotein (114 residues) has been characterized by heteronuclear multidimensional NMR spectroscopy. Sequence-specific backbone atom resonance assignments were obtained for a uniformly 13C/15N-enriched sample of apokedarcidin via a semiautomated analysis of 3D HNCACB, 3D CBCA-(CO)NH, 4D HNCAHA, 4D HN(CO)CAHA, 3D HBHA(CO)NH, and 3D HNHA(Gly) spectra. Side-chain assignments were subsequently obtained by analysis of (primarily) 3D HCCH-TOCSY and HCCH-COSY spectra. A qualitative analysis of the secondary structure is presented on the basis of 3J alpha NH coupling constants, deviations of 13C alpha and 13C beta chemical shifts from random coil values, and NOEs observed in 3D 15N- and 13C-edited NOESY-HSQC spectra. This analysis revealed a four-stranded antiparallel beta-sheet, a three-stranded antiparallel beta-sheet, and two two-standed antiparallel beta-sheets. The assignments of cross-peaks in the 3D NOESY spectra were assisted by reference to a preliminary model of apokedarcidin built using the program CONGEN starting from the X-ray structure of the homologous protein aponeocarzinostatin. An ensemble of 15 apokedarcidin solution structures has been generated by variable target function minimization (DIANA program) and refined by simulated annealing (X-PLOR program). The average backbone atom root-mean-square difference between the individual structures and the mean coordinates is 0.68 +/- 0.08 A. The overall fold of apokedarcidin is well-defined; it is composed of an immunoglobulin-like seven-stranded antiparallel beta-barrel and a subdomain containing two antiparallel beta-ribbons. Highly similar tertiary structures have been previously reported for the related proteins neocarzinostatin, macromomycin, and actinoxanthin. Important structural features are revealed, including the dimensions of the chromophore-binding pocket and the locations of side chains that are likely to be involved in chromophore stabilization.
Solid-state NMR sequential assignments of ?-synuclein.
Solid-state NMR sequential assignments of ?-synuclein.
Solid-state NMR sequential assignments of ?-synuclein.
Biomol NMR Assign. 2011 Jul 9;
Authors: Gath J, Habenstein B, Bousset L, Melki R, Meier BH, Böckmann A
Parkinson's disease is amongst the most frequent and most devastating neurodegenerative diseases. It is tightly associated with the assembly of proteins into high-molecular weight protein species, which propagate between neurons in the central nervous system. The principal protein involved in this process is ?-synuclein which is a...
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[NMR paper] Proton NMR assignments and solution conformation of RANTES, a chemokine of the C-C ty
Proton NMR assignments and solution conformation of RANTES, a chemokine of the C-C type.
Related Articles Proton NMR assignments and solution conformation of RANTES, a chemokine of the C-C type.
Biochemistry. 1995 Apr 25;34(16):5329-42
Authors: Skelton NJ, Aspiras F, Ogez J, Schall TJ
1H NMR has been used to investigate the structural properties of RANTES, a protein from the C-C branch of the chemotactic cytokine family that has a strong chemoattractive effect on monocytes, lymphocytes, and eosinophils. Titration of pH from 5.0 to 2.5...
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[NMR paper] NMR sequential assignments and solution structure of chlorotoxin, a small scorpion to
NMR sequential assignments and solution structure of chlorotoxin, a small scorpion toxin that blocks chloride channels.
Related Articles NMR sequential assignments and solution structure of chlorotoxin, a small scorpion toxin that blocks chloride channels.
Biochemistry. 1995 Jan 10;34(1):13-21
Authors: Lippens G, Najib J, Wodak SJ, Tartar A
The solution structure of chlorotoxin, a small toxin purified from the venom of the Leiurus quinquestriatus scorpion, has been determined using 2D 1H NMR spectroscopy. Analysis of the NMR data shows that...
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[NMR paper] Sequential 1H and 15N NMR assignments and secondary structure of a recombinant anti-d
Sequential 1H and 15N NMR assignments and secondary structure of a recombinant anti-digoxin antibody VL domain.
Related Articles Sequential 1H and 15N NMR assignments and secondary structure of a recombinant anti-digoxin antibody VL domain.
Biochemistry. 1992 Jun 2;31(21):5033-43
Authors: Constantine KL, Goldfarb V, Wittekind M, Anthony J, Ng SC, Mueller L
A uniformly 15N-labeled recombinant light-chain variable (VL) domain from the anti-digoxin antibody 26-10 has been investigated by heteronuclear two-dimensional (2D) and three-dimensional...
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[NMR paper] 1H NMR sequential assignments and identification of secondary structural elements in
1H NMR sequential assignments and identification of secondary structural elements in oxidized putidaredoxin, an electron-transfer protein from Pseudomonas.
Related Articles 1H NMR sequential assignments and identification of secondary structural elements in oxidized putidaredoxin, an electron-transfer protein from Pseudomonas.
Biochemistry. 1992 Feb 25;31(7):1961-8
Authors: Ye XM, Pochapsky TC, Pochapsky SS
Sequential 1H resonance assignments and secondary structural features of putidaredoxin (Pdx), a 106-residue globular protein consisting of...
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[NMR paper] Dihydrofolate reductase: sequential resonance assignments using 2D and 3D NMR and sec
Dihydrofolate reductase: sequential resonance assignments using 2D and 3D NMR and secondary structure determination in solution.
Related Articles Dihydrofolate reductase: sequential resonance assignments using 2D and 3D NMR and secondary structure determination in solution.
Biochemistry. 1991 Jun 25;30(25):6330-41
Authors: Carr MD, Birdsall B, Frenkiel TA, Bauer CJ, Jimenez-Barbero J, Polshakov VI, McCormick JE, Roberts GC, Feeney J
Three-dimensional (3D) heteronuclear NMR techniques have been used to make sequential 1H and 15N resonance...
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[NMR paper] 1H NMR studies of echistatin in solution. Sequential resonance assignments and second
1H NMR studies of echistatin in solution. Sequential resonance assignments and secondary structure.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif Related Articles 1H NMR studies of echistatin in solution. Sequential resonance assignments and secondary structure.
Eur J Biochem. 1991 Dec 5;202(2):315-21
Authors: Dalvit C, Widmer H, Bovermann G, Breckenridge R, Metternich R
Two-dimensional 1H-NMR methods have been used to obtain complete proton...
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[NMR paper] 1H NMR studies of echistatin in solution. Sequential resonance assignments and second
1H NMR studies of echistatin in solution. Sequential resonance assignments and secondary structure.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif Related Articles 1H NMR studies of echistatin in solution. Sequential resonance assignments and secondary structure.
Eur J Biochem. 1991 Dec 5;202(2):315-21
Authors: Dalvit C, Widmer H, Bovermann G, Breckenridge R, Metternich R
Two-dimensional 1H-NMR methods have been used to obtain complete proton...