NMR paper Sequence-specific 1H NMR assignments and secondary structure of the streptococcal pro

		BioNMR > Educational resources > Journal club
[NMR paper] Sequence-specific 1H NMR assignments and secondary structure of the streptococcal pro

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

08-21-2010, 11:41 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Sequence-specific 1H NMR assignments and secondary structure of the streptococcal pro

Sequence-specific 1H NMR assignments and secondary structure of the streptococcal protein G B2-domain.

Related Articles Sequence-specific 1H NMR assignments and secondary structure of the streptococcal protein G B2-domain.

Biochemistry. 1992 Apr 14;31(14):3604-11

Authors: Orban J, Alexander P, Bryan P

Two-dimensional NMR spectroscopy has been used to obtain sequence-specific 1H NMR assignments for the IgG-binding B2-domain of streptococcal protein G. Secondary structure elements were identified from analysis of characteristic backbone-backbone NOE patterns and amide proton exchange data. The B2-domain contains a four-stranded beta-sheet region in which the two inner strands form a parallel beta-sheet with each other and antiparallel beta-sheets with the outer strands. The outer strands are connected via a 16-residue alpha-helix and short loops on both ends of the helix. The alpha-helix and beta-sheet structures contain well-defined polar and apolar sides, and numerous long-range NOEs from the apolar helix to apolar sheet regions were used to derive a model for the global fold of the B2-domain. While the overall fold is similar to that obtained for B1-type domains, differences in amide proton exchange rates and hydrophobic packing are observed.

PMID: 1314644 [PubMed - indexed for MEDLINE]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] Sequence-specific 1H-NMR assignments and secondary structure of the lipoyl domain of Sequence-specific 1H-NMR assignments and secondary structure of the lipoyl domain of the Bacillus stearothermophilus pyruvate dehydrogenase multienzyme complex. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif Related Articles Sequence-specific 1H-NMR assignments and secondary structure of the lipoyl domain of the Bacillus stearothermophilus pyruvate dehydrogenase multienzyme complex. Eur J Biochem. 1991 Oct 1;201(1):203-9 Authors: Dardel F, Laue ED, Perham RN...	nmrlearner	Journal club	0	08-21-2010 11:12 PM
[NMR paper] Sequence-specific 1H-NMR assignments and secondary structure of the lipoyl domain of Sequence-specific 1H-NMR assignments and secondary structure of the lipoyl domain of the Bacillus stearothermophilus pyruvate dehydrogenase multienzyme complex. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif Related Articles Sequence-specific 1H-NMR assignments and secondary structure of the lipoyl domain of the Bacillus stearothermophilus pyruvate dehydrogenase multienzyme complex. Eur J Biochem. 1991 Oct 1;201(1):203-9 Authors: Dardel F, Laue ED, Perham RN...	nmrlearner	Journal club	0	08-21-2010 11:12 PM
[NMR paper] Sequence-specific 1H NMR assignments, secondary structure, and location of the calciu Sequence-specific 1H NMR assignments, secondary structure, and location of the calcium binding site in the first epidermal growth factor like domain of blood coagulation factor IX. Related Articles Sequence-specific 1H NMR assignments, secondary structure, and location of the calcium binding site in the first epidermal growth factor like domain of blood coagulation factor IX. Biochemistry. 1991 Jul 30;30(30):7402-9 Authors: Huang LH, Cheng H, Pardi A, Tam JP, Sweeney WV Factor IX is a blood clotting protein that contains three regions,...	nmrlearner	Journal club	0	08-21-2010 11:12 PM
[NMR paper] Sequence-specific 1H NMR assignments, secondary structure, and location of the calciu Sequence-specific 1H NMR assignments, secondary structure, and location of the calcium binding site in the first epidermal growth factor like domain of blood coagulation factor IX. Related Articles Sequence-specific 1H NMR assignments, secondary structure, and location of the calcium binding site in the first epidermal growth factor like domain of blood coagulation factor IX. Biochemistry. 1991 Jul 30;30(30):7402-9 Authors: Huang LH, Cheng H, Pardi A, Tam JP, Sweeney WV Factor IX is a blood clotting protein that contains three regions,...	nmrlearner	Journal club	0	08-21-2010 11:12 PM
[NMR paper] Sequence-specific 1H NMR assignments and determination of the secondary structure for Sequence-specific 1H NMR assignments and determination of the secondary structure for the activation domain isolated from pancreatic procarboxypeptidase B. Related Articles Sequence-specific 1H NMR assignments and determination of the secondary structure for the activation domain isolated from pancreatic procarboxypeptidase B. Biochemistry. 1990 Aug 14;29(32):7515-22 Authors: Vendrell J, Wider G, Avilés FX, Wüthrich K Nearly complete sequence-specific 1H NMR assignments are presented for amino acid residues 3-81 in the 81-residue globular...	nmrlearner	Journal club	0	08-21-2010 11:04 PM
[NMR paper] Sequence-specific 1H NMR assignments and secondary structure in solution of Escherich Sequence-specific 1H NMR assignments and secondary structure in solution of Escherichia coli trp repressor. Related Articles Sequence-specific 1H NMR assignments and secondary structure in solution of Escherichia coli trp repressor. Biochemistry. 1990 Jul 10;29(27):6332-41 Authors: Arrowsmith CH, Pachter R, Altman RB, Iyer SB, Jardetzky O Sequence-specific 1H NMR assignments are reported for the active L-tryptophan-bound form of Escherichia coli trp repressor. The repressor is a symmetric dimer of 107 residues per monomer; thus at 25 kDa, this...	nmrlearner	Journal club	0	08-21-2010 11:04 PM
[NMR paper] Sequence-specific [1H]NMR resonance assignments and secondary structure identificatio Sequence-specific NMR resonance assignments and secondary structure identification for 1- and 2-zinc finger constructs from SW15. A hydrophobic core involving four invariant residues. Related Articles Sequence-specific NMR resonance assignments and secondary structure identification for 1- and 2-zinc finger constructs from SW15. A hydrophobic core involving four invariant residues. FEBS Lett. 1990 Mar 26;262(2):179-84 Authors: Neuhaus D, Nakaseko Y, Nagai K, Klug A Complete NMR resonance assignments are presented for the second of the three...	nmrlearner	Journal club	0	08-21-2010 10:48 PM
[NMR paper] Sequence-specific 1H NMR assignments and secondary structure of eglin c. Sequence-specific 1H NMR assignments and secondary structure of eglin c. Related Articles Sequence-specific 1H NMR assignments and secondary structure of eglin c. Biochemistry. 1990 Feb 13;29(6):1465-74 Authors: Hyberts SG, Wagner G Sequence-specific nuclear magnetic resonance assignments were obtained for eglin c, a polypeptide inhibitor of the granulocytic proteinases elastase and cathepsin G and some other proteinases. The protein consists of a single polypeptide chain of 70 residues. All proton resonances were assigned except for some...	nmrlearner	Journal club	0	08-21-2010 10:48 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off