Related ArticlesSensitivity-enhanced solid-state NMR detection of expansin's target in plant cell walls.
Proc Natl Acad Sci U S A. 2013 Sep 24;
Authors: Wang T, Park YB, Caporini MA, Rosay M, Zhong L, Cosgrove DJ, Hong M
Abstract
Structure determination of protein binding to noncrystalline macromolecular assemblies such as plant cell walls (CWs) poses a significant structural biology challenge. CWs are loosened during growth by expansin proteins, which weaken the noncovalent network formed by cellulose, hemicellulose, and pectins, but the CW target of expansins has remained elusive because of the minute amount of the protein required for activity and the complex nature of the CW. Using solid-state NMR spectroscopy, combined with sensitivity-enhancing dynamic nuclear polarization (DNP) and differential isotopic labeling of expansin and polysaccharides, we have now determined the functional binding target of expansin in the Arabidopsis thaliana CW. By transferring the electron polarization of a biradical dopant to the nuclei, DNP allowed selective detection of (13)C spin diffusion from trace concentrations of (13)C, (15)N-labeled expansin in the CW to nearby polysaccharides. From the spin diffusion data of wild-type and mutant expansins, we conclude that to loosen the CW, expansin binds highly specific cellulose domains enriched in xyloglucan, whereas more abundant binding to pectins is unrelated to activity. Molecular dynamics simulations indicate short (13)C-(13)C distances of 4-6 Å between a hydrophobic surface of the cellulose microfibril and an aromatic motif on the expansin surface, consistent with the observed NMR signals. DNP-enhanced 2D (13)C correlation spectra further reveal that the expansin-bound cellulose has altered conformation and is enriched in xyloglucan, thus providing unique insight into the mechanism of CW loosening. DNP-enhanced NMR provides a powerful, generalizable approach for investigating protein binding to complex macromolecular targets.
PMID: 24065828 [PubMed - as supplied by publisher]
Solid-State NMR Investigations of Peptide-Lipid Interactions of the Transmembrane Domain of A Plant-Derived Protein, Hcf106
Solid-State NMR Investigations of Peptide-Lipid Interactions of the Transmembrane Domain of A Plant-Derived Protein, Hcf106
Publication date: Available online 24 September 2013
Source:Chemistry and Physics of Lipids</br>
Author(s): Lei Zhang , Lishan Liu , Sergey Maltsev , Gary A. Lorigan , Carole Dabney-Smith</br>
The chloroplast twin arginine translocation system transports highly folded precursor proteins across the thylakoid using the protonmotive force as its only energy source. Hcf106 and another thylakoid protein, cpTatC compose the precursor receptor...
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09-25-2013 11:15 AM
Dynamic Nuclear Polarization Enhanced NMR in the Solid-State
From The DNP-NMR Blog:
Dynamic Nuclear Polarization Enhanced NMR in the Solid-State
Akbey, Ü., et al., Dynamic Nuclear Polarization Enhanced NMR in the Solid-State. 2013, Springer Berlin Heidelberg. p. 1-48.
http://dx.doi.org/10.1007/128_2013_436
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08-26-2013 08:46 PM
[NMR paper] Sensitivity and Resolution Enhanced Solid-State NMR for Paramagnetic Systems and Biomolecules under Very Fast Magic Angle Spinning.
Sensitivity and Resolution Enhanced Solid-State NMR for Paramagnetic Systems and Biomolecules under Very Fast Magic Angle Spinning.
Related Articles Sensitivity and Resolution Enhanced Solid-State NMR for Paramagnetic Systems and Biomolecules under Very Fast Magic Angle Spinning.
Acc Chem Res. 2013 Jul 26;
Authors: Parthasarathy S, Nishiyama Y, Ishii Y
Abstract
Recent research in fast magic angle spinning (MAS) methods has drasticallyimproved the resolution and sensitivity of NMR spectroscopy of biomolecules and materials in solids. In...
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07-31-2013 12:00 PM
[NMR paper] Dynamic Nuclear Polarization Enhanced NMR in the Solid-State.
Dynamic Nuclear Polarization Enhanced NMR in the Solid-State.
Dynamic Nuclear Polarization Enhanced NMR in the Solid-State.
Top Curr Chem. 2013 Jul 7;
Authors: Akbey U, Franks WT, Linden A, Rydmark MO, Lange S, Oschkinat H
Abstract
Nuclear magnetic resonance (NMR) spectroscopy is one of the most commonly used spectroscopic techniques to obtain information on the structure and dynamics of biological and chemical materials. A variety of samples can be studied including solutions, crystalline solids, powders and hydrated protein...
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07-09-2013 02:47 PM
Structure and Interactions of Plant Cell-Wall Polysaccharides by Two- and Three-Dimensional Magic-Angle-Spinning Solid-State NMR
Structure and Interactions of Plant Cell-Wall Polysaccharides by Two- and Three-Dimensional Magic-Angle-Spinning Solid-State NMR
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/bi101795q/aop/images/medium/bi-2010-01795q_0008.gif
Biochemistry
DOI: 10.1021/bi101795q
http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/bichaw/~4/9XLFFfb1pRU
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01-21-2011 03:31 AM
[NMR paper] Sensitivity enhancement in two-dimensional solid-state NMR spectroscopy by transverse
Sensitivity enhancement in two-dimensional solid-state NMR spectroscopy by transverse mixing.
Related Articles Sensitivity enhancement in two-dimensional solid-state NMR spectroscopy by transverse mixing.
Chemphyschem. 2004 Jun 21;5(6):863-8
Authors: Tycko R
The sensitivity of two-dimensional (2D) 13C-13C solid-state NMR spectroscopy under magic-angle spinning (MAS) is shown to be enhanced by the use of transverse polarization transfer in place of the conventional longitudinal polarization transfer. Experimental results are reported for 2D...
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11-24-2010 09:51 PM
[NMR paper] Sensitivity-enhanced static 15N NMR of solids by 1h indirect detection.
Sensitivity-enhanced static 15N NMR of solids by 1h indirect detection.
Related Articles Sensitivity-enhanced static 15N NMR of solids by 1h indirect detection.
J Magn Reson. 2001 May;150(1):43-8
Authors: Hong M, Yamaguchi S
A method for enhancing the sensitivity of 15N spectra of nonspinning solids through 1H indirect detection is introduced. By sampling the 1H signals in the windows of a pulsed spin-lock sequence, high-sensitivity 1H spectra can be obtained in two-dimensional (2D) spectra whose indirect dimension yields the 15N chemical...
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11-19-2010 08:32 PM
[NMR paper] Direct observation of cell wall structure in living plant tissues by solid-state C NM
Direct observation of cell wall structure in living plant tissues by solid-state C NMR spectroscopy.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles Direct observation of cell wall structure in living plant tissues by solid-state C NMR spectroscopy.
Plant Physiol. 1990 Jan;92(1):61-5
Authors: Jarvis MC, Apperley DC
Solid-state (13)C nuclear magnetic resonance (NMR) spectra of the following intact plant tissues were recorded by the crosspolarization...