NMR paper Sensitive and simplified: a combinatorial acquisition of five distinct 2D constant-time 13C-1H NMR protein correlation spectra.

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[NMR paper] Sensitive and simplified: a combinatorial acquisition of five distinct 2D constant-time 13C-1H NMR protein correlation spectra.

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08-19-2020, 09:17 AM

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Sensitive and simplified: a combinatorial acquisition of five distinct 2D constant-time 13C-1H NMR protein correlation spectra.

Sensitive and simplified: a combinatorial acquisition of five distinct 2D constant-time 13C-1H NMR protein correlation spectra.

Related Articles Sensitive and simplified: a combinatorial acquisition of five distinct 2D constant-time 13C-1H NMR protein correlation spectra.

J Biomol NMR. 2020 Aug 17;:

Authors: Yoshimura Y, Mulder FAA

Abstract
A procedure is presented for the substantial simplification of 2D constant-time 13C-1H heteronuclear single-quantum correlation (HSQC) spectra of 13C-enriched proteins. In this approach, a single pulse sequence simultaneously records eight sub-spectra wherein the phases of the NMR signals depend on spin topology. Signals from different chemical groups are then stratified into different sub-spectra through linear combination based on Hadamard encoding of 13CHn multiplicity (n = 1, 2, and 3) and the chemical nature of neighboring 13C nuclei (aliphatic, carbonyl/carboxyl, aromatic). This results in five sets of 2D NMR spectra containing mutually exclusive signals from: (i) 13C?-1H? correlations of asparagine and aspartic acid, 13C?-1H? correlations of glutamine and glutamic acid, and 13C?-1H? correlations of glycine, (ii) 13C?-1H? correlations of all residues but glycine, and (iii) 13C?-1H? correlations of phenylalanine, tyrosine, histidine, and tryptophan, and the remaining (iv) aliphatic 13CH2 and (v) aliphatic 13CH/13CH3 resonances. As HSQC is a common element of many NMR experiments, the spectral simplification proposed in this article can be straightforwardly implemented in experiments for resonance assignment and structure determination and should be of widespread utility.

PMID: 32804297 [PubMed - as supplied by publisher]

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