Translational Diffusion of Macromolecular Assemblies Measured Using Transverse-Relaxation-Optimized Pulsed Field Gradient NMR
Translational Diffusion of Macromolecular Assemblies Measured Using Transverse-Relaxation-Optimized Pulsed Field Gradient NMR
Reto Horst, Arthur L. Horwich and Kurt Wu?thrich
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja206531c/aop/images/medium/ja-2011-06531c_0003.gif
Journal of the American Chemical Society
DOI: 10.1021/ja206531c
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Probing Microscopic Architecture of Opaque Heterogeneous Systems Using Double-Pulsed-Field-Gradient NMR
Probing Microscopic Architecture of Opaque Heterogeneous Systems Using Double-Pulsed-Field-Gradient NMR
Noam Shemesh, Tal Adiri and Yoram Cohen
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja200303h/aop/images/medium/ja-2011-00303h_0005.gif
Journal of the American Chemical Society
DOI: 10.1021/ja200303h
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03-30-2011 09:10 AM
Spin Echo Diagram.png
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02-08-2011 08:16 PM
[NMR paper] Slow diffusion of macromolecular assemblies by a new pulsed field gradient NMR method
Slow diffusion of macromolecular assemblies by a new pulsed field gradient NMR method.
Related Articles Slow diffusion of macromolecular assemblies by a new pulsed field gradient NMR method.
J Am Chem Soc. 2003 Mar 5;125(9):2541-5
Authors: Ferrage F, Zoonens M, Warschawski DE, Popot JL, Bodenhausen G
The translational diffusion coefficient of an integral membrane protein/surfactant complex has been measured using a novel pulsed field gradient NMR method. In this new approach, the information about the localization of the molecules is...
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11-24-2010 09:01 PM
[NMR paper] Improved spin-echo-edited NMR diffusion measurements.
Improved spin-echo-edited NMR diffusion measurements.
Related Articles Improved spin-echo-edited NMR diffusion measurements.
J Magn Reson. 2001 Dec;153(2):273-6
Authors: Otto WH, Larive CK
The need for simple and robust schemes for the analysis of ligand-protein binding has resulted in the development of diffusion-based NMR techniques that can be used to assay binding in protein solutions containing a mixture of several ligands. As a means of gaining spectral selectivity in NMR diffusion measurements, a simple experiment, the gradient modified...
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11-19-2010 08:44 PM
[NMR paper] 1H NMR Self-Diffusion in Polymer-Surfactant Nanocapsules and Cryogels with Enzyme.
1H NMR Self-Diffusion in Polymer-Surfactant Nanocapsules and Cryogels with Enzyme.
Related Articles 1H NMR Self-Diffusion in Polymer-Surfactant Nanocapsules and Cryogels with Enzyme.
J Colloid Interface Sci. 1998 Oct 1;206(1):168-176
Authors: Shapiro YE, Pykhteeva EG, Levashov AV
The multicomponent self-diffusion in nanocapsules and cryogel biocatalytic systems containing alpha-chymotrypsin has been studied with the NMR-PGSE method at various temperatures and compared with the diffusion of such systems without enzyme. Unilamellar vesicles have...
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11-17-2010 11:15 PM
[NMR paper] A pulsed field gradient NMR study of the aggregation and hydration of parvalbumin.
A pulsed field gradient NMR study of the aggregation and hydration of parvalbumin.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles A pulsed field gradient NMR study of the aggregation and hydration of parvalbumin.
Biophys Chem. 1997 Apr 22;65(2-3):179-87
Authors: Price WS, Nara M, Arata Y
Pulsed field gradient NMR is a convenient alternative to traditional methods for measuring diffusion of biological macromolecules. In the present study, pulsed field gradient NMR was...
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08-22-2010 03:31 PM
[U. of Ottawa NMR Facility Blog] Gradient Spin Echoes for Selective Excitation
Gradient Spin Echoes for Selective Excitation
Shaped excitation pulses can replace the non-selective hard pulses typically used in a one-pulse measurement to achieve selective excitation. Another method of achieving selective excitation is the gradient spin echo using a selective 180° pulse. This technique is demonstrated in the figure below. http://4.bp.blogspot.com/_5wBTR2kKTqA/S_UxeG5oXdI/AAAAAAAAAzc/BHWef-Tse7s/s400/grad_spin_echo.jpgA non-selective hard 90°x pulse is first given followed by a pair of identical pulsed field gradients sandwiching a soft selective 180° pulse about the y...