Related ArticlesSelf-Assembly of DNA and RNA Building Blocks Explored by Nitrogen-14 NMR Crystallography: Structure and Dynamics.
Chemphyschem. 2020 Mar 19;:
Authors: Carnevale D, Hollenstein M, Bodenhausen G
Abstract
The isotopic enrichment of nucleic acids with nitrogen-15 is often carried out by solid-phase synthesis of oligonucleotides using phosphoramidites precursors that are synthetically demanding and expensive. These synthetic challenges, combined with the overlap of chemical shifts, explain the lag of nitrogen-15 NMR studies of nucleic acids behind those of proteins. For the structural characterization of DNA and RNA-related systems, new NMR methods that exploit the naturally occurring 99.9% abundant nitrogen-14 isotope are therefore highly desirable. In this study, we have investigated nitrogen-14 spectra of self-assembled quartets based on the nucleobase guanine in the solid state by means of magic-angle spinning NMR spectroscopy. The network of dipolar proton-nitrogen couplings between neighboring stacked purine units is probed by 2D spectra based on 1H®14N®1H double cross-polarization. Interplane dipolar contacts are identified between the stacked G quartets. The assignment is supported by density functional theory (DFT) calculations of the anisotropic chemical shifts and quadrupolar parameters. The experimental spectra are fully consistent with internuclear distances obtained in silico. Averaging of chemical shifts due to internal motions can be interpreted by semiempirical calculations. This method can easily be extended to synthetic G quartets based on nucleobase or nucleoside analogs and potentially to oligonucleotides.
PMID: 32191377 [PubMed - as supplied by publisher]
[ASAP] Proteins as Sustainable Building Blocks for the Next Generation of Bioinorganic Nanomaterials
Proteins as Sustainable Building Blocks for the Next Generation of Bioinorganic Nanomaterials
https://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.8b00966/20181003/images/medium/bi-2018-00966a_0001.gif
Biochemistry
DOI: 10.1021/acs.biochem.8b00966
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11-25-2018 06:02 AM
[NMR paper] Synthesis and incorporation of 13C-labeled DNA building blocks to probe structural dynamics of DNA by NMR.
Synthesis and incorporation of 13C-labeled DNA building blocks to probe structural dynamics of DNA by NMR.
Related Articles Synthesis and incorporation of 13C-labeled DNA building blocks to probe structural dynamics of DNA by NMR.
Nucleic Acids Res. 2017 Sep 06;45(15):9178-9192
Authors: Nußbaumer F, Juen MA, Gasser C, Kremser J, Müller T, Tollinger M, Kreutz C
Abstract
We report the synthesis of atom-specifically 13C-modified building blocks that can be incorporated into DNA via solid phase synthesis to facilitate investigations...
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09-16-2017 09:58 PM
Protein Assembly and Building Blocks: Beyond the Limitsof the LEGO Brick Metaphor
Protein Assembly and Building Blocks: Beyond the Limitsof the LEGO Brick Metaphor
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.7b00666/20170830/images/medium/bi-2017-006667_0004.gif
Biochemistry
DOI: 10.1021/acs.biochem.7b00666
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09-01-2017 05:57 AM
Interdomain Dynamics Explored by Paramagnetic NMR
Interdomain Dynamics Explored by Paramagnetic NMR
Luigi Russo, Mitcheell Maestre-Martinez, Sebastian Wolff, Stefan Becker and Christian Griesinger
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja408143f/aop/images/medium/ja-2013-08143f_0006.gif
Journal of the American Chemical Society
DOI: 10.1021/ja408143f
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11-05-2013 06:34 AM
[NMR paper] Inter-domain dynamics explored by paramagnetic NMR.
Inter-domain dynamics explored by paramagnetic NMR.
Related Articles Inter-domain dynamics explored by paramagnetic NMR.
J Am Chem Soc. 2013 Oct 11;
Authors: Russo L, Maestre-Martínez M, Wolff S, Becker S, Griesinger C
Abstract
An ensemble-based approach is presented to explore the conformational space sampled by a multi-domain protein showing moderate inter-domain dynamics in terms of translational and rotational motions. The strategy was applied on a complex of calmodulin (CaM) with the IQ-recognition motif from the voltage-gated calcium...
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10-12-2013 05:24 PM
[NMR paper] Monitoring Binding of HIV-1 Capsid Assembly Inhibitors Using (19) F Ligand-and (15) N Protein-Based NMR and X-ray Crystallography: Early Hit Validation of a Benzodiazepine Series.
Monitoring Binding of HIV-1 Capsid Assembly Inhibitors Using (19) F Ligand-and (15) N Protein-Based NMR and X-ray Crystallography: Early Hit Validation of a Benzodiazepine Series.
Related Articles Monitoring Binding of HIV-1 Capsid Assembly Inhibitors Using (19) F Ligand-and (15) N Protein-Based NMR and X-ray Crystallography: Early Hit Validation of a Benzodiazepine Series.
ChemMedChem. 2013 Feb 10;
Authors: Goudreau N, Coulombe R, Faucher AM, Grand-Maître C, Lacoste JE, Lemke CT, Malenfant E, Bousquet Y, Fader L, Simoneau B, Mercier JF, Titolo S, Mason SW
...
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02-13-2013 12:47 PM
Combination of NMR spectroscopy and X-ray crystallography offers unique advantages for elucidation of the structural basis of protein complex assembly.
Combination of NMR spectroscopy and X-ray crystallography offers unique advantages for elucidation of the structural basis of protein complex assembly.
Combination of NMR spectroscopy and X-ray crystallography offers unique advantages for elucidation of the structural basis of protein complex assembly.
Sci China Life Sci. 2011 Feb;54(2):101-11
Authors: Feng W, Pan L, Zhang M
NMR spectroscopy and X-ray crystallography are two premium methods for determining the atomic structures of macro-biomolecular complexes. Each method has unique strengths and...
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02-15-2011 07:17 PM
[NMR paper] Structure and dynamics of a protein assembly. 1H-NMR studies of the 36 kDa R6 insulin
Structure and dynamics of a protein assembly. 1H-NMR studies of the 36 kDa R6 insulin hexamer.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Structure and dynamics of a protein assembly. 1H-NMR studies of the 36 kDa R6 insulin hexamer.
J Mol Biol. 1996 Apr 26;258(1):136-57
Authors: Jacoby E, Hua QX, Stern AS, Frank BH, Weiss MA
The structure and dynamics of the R6 human insulin hexamer are investigated by two- and three-dimensional homonuclear 1H-NMR spectroscopy....