Selective detection of biomolecules and their modifications in cells is essential for understanding cell functions and diseases. We have developed an NMR pulse sequence, Ac-FIND (Acetylation-FIltered aNd eDited), which uses isotope editing/filtering techniques for selective detection of protein acetylation. Acetylation of the N-terminus and lysine side chains by N-succinimidyl acetate was selectively observed for intrinsically disordered ?-synuclein and well-ordered ubiquitin. Furthermore, when...
[NMR paper] Target-specific NMR detection of protein-ligand interactions with antibody-relayed (15)N-group selective STD.
Target-specific NMR detection of protein-ligand interactions with antibody-relayed (15)N-group selective STD.
Target-specific NMR detection of protein-ligand interactions with antibody-relayed (15)N-group selective STD.
J Biomol NMR. 2016 Nov 24;
Authors: Hetényi A, Heged?s Z, Fajka-Boja R, Monostori É, Kövér KE, Martinek TA
Abstract
Fragment-based drug design has been successfully applied to challenging targets where the detection of the weak protein-ligand interactions is a key element. (1)H saturation transfer difference...
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11-26-2016 12:27 PM
Target-specific NMR detection of proteinâ??ligand interactions with antibody-relayed 15 N-group selective STD
Target-specific NMR detection of proteinâ??ligand interactions with antibody-relayed 15 N-group selective STD
Abstract
Fragment-based drug design has been successfully applied to challenging targets where the detection of the weak proteinâ??ligand interactions is a key element. 1H saturation transfer difference (STD) NMR spectroscopy is a powerful technique for this work but it requires pure homogeneous proteins as targets. Monoclonal antibody (mAb)-relayed 15N-GS STD spectroscopy has been developed to resolve the problem of protein mixtures and...
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11-25-2016 02:22 AM
Selective High-Resolution Detection of Membrane Protein-Ligand Interaction in Native Membranes Using Trityl-Nitroxide PELDOR
From The DNP-NMR Blog:
Selective High-Resolution Detection of Membrane Protein-Ligand Interaction in Native Membranes Using Trityl-Nitroxide PELDOR
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Joseph, B., et al., Selective High-Resolution Detection of Membrane Protein-Ligand Interaction in Native Membranes Using Trityl-Nitroxide PELDOR. Angew Chem Int Ed Engl, 2016. 55(38): p. 11538-42.
https://www.ncbi.nlm.nih.gov/pubmed/27511025
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11-19-2016 08:35 PM
[NMR paper] NMR analysis of the acetylation pattern of the neuronal Tau protein.
NMR analysis of the acetylation pattern of the neuronal Tau protein.
NMR analysis of the acetylation pattern of the neuronal Tau protein.
Biochemistry. 2014 Apr 7;
Authors: Kamah A, Huvent I, Cantrelle FX, Qi H, Lippens G, Landrieu I, Smet-Nocca C
Abstract
Lysine acetylation of the neuronal Tau protein was described as a novel mechanism of posttranslational regulation of Tau functions with important outcomes in microtubule binding and aggregation processes related to Alzheimer's disease. Here, we unravel at a per-residue...
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04-09-2014 10:40 AM
[NMR paper] Selective interface detection: mapping binding site contacts in membrane proteins by
Selective interface detection: mapping binding site contacts in membrane proteins by NMR spectroscopy.
Related Articles Selective interface detection: mapping binding site contacts in membrane proteins by NMR spectroscopy.
J Am Chem Soc. 2005 Apr 27;127(16):5734-5
Authors: Kiihne SR, Creemers AF, de Grip WJ, Bovee-Geurts PH, Lugtenburg J, de Groot HJ
Intermolecular contact surfaces are important regions where specific interactions mediate biological function. We introduce a new magic angle spinning solid state NMR technique, dubbed "selective...
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11-25-2010 08:21 PM
Simultaneous Detection of Protein Phosphorylation and Acetylation by High-Resolution
Simultaneous Detection of Protein Phosphorylation and Acetylation by High-Resolution NMR Spectroscopy.
Simultaneous Detection of Protein Phosphorylation and Acetylation by High-Resolution NMR Spectroscopy.
J Am Chem Soc. 2010 Oct 1;
Authors: Liokatis S, Dose A, Schwarzer D, Selenko P
Post-translational protein modifications (PTMs) such as phosphorylation and acetylation regulate a large number of eukaryotic signaling processes. In most instances, it is the combination of different PTMs that "encode" the biological outcome of these covalent...
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10-05-2010 12:11 PM
Simultaneous Detection of Protein Phosphorylation and Acetylation by High-Resolution
Simultaneous Detection of Protein Phosphorylation and Acetylation by High-Resolution NMR Spectroscopy
Stamatios Liokatis, Alexander Dose, Dirk Schwarzer and Philipp Selenko
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja106764y/aop/images/medium/ja-2010-06764y_0002.gif
Journal of the American Chemical Society
DOI: 10.1021/ja106764y
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/jfhp2Sg-hpY
Selective detection of protein acetylation by NMR spectroscopy
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