NMR paper Secondary structure of human interleukin 2 from 3D heteronuclear NMR experiments.

		BioNMR > Educational resources > Journal club
[NMR paper] Secondary structure of human interleukin 2 from 3D heteronuclear NMR experiments.

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

08-21-2010, 11:45 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Secondary structure of human interleukin 2 from 3D heteronuclear NMR experiments.

Secondary structure of human interleukin 2 from 3D heteronuclear NMR experiments.

Related Articles Secondary structure of human interleukin 2 from 3D heteronuclear NMR experiments.

Biochemistry. 1992 Aug 25;31(33):7741-4

Authors: Mott HR, Driscoll PC, Boyd J, Cooke RM, Weir MP, Campbell ID

Recombinant 15N-labeled human interleukin 2 (IL-2) has been studied by 2D and 3D NMR using uniformly 15N-labeled protein. Assignment of the backbone resonances has enabled the secondary structure of the protein to be defined. The secondary structure was found to consist of four alpha-helical regions and a short section of antiparallel beta-sheet. This structure is more similar to recent published structures of interleukin 4 and granulocyte-macrophage colony-stimulating factor than to a structure of IL-2 previously obtained from low-resolution X-ray diffraction data.

PMID: 1510960 [PubMed - indexed for MEDLINE]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] Direct determination of the interleukin-6 binding epitope of the interleukin-6 recept Direct determination of the interleukin-6 binding epitope of the interleukin-6 receptor by NMR spectroscopy. Related Articles Direct determination of the interleukin-6 binding epitope of the interleukin-6 receptor by NMR spectroscopy. J Biol Chem. 2004 Jan 2;279(1):571-6 Authors: Schwantner A, Dingley AJ, Ozbek S, Rose-John S, Grötzinger J All cytokines belonging to the interleukin-6 (IL-6)-type family of cytokines utilize receptors that have a modular build of several immunoglobulin-like and fibronectin type III-like domains. Characteristic...	nmrlearner	Journal club	0	11-24-2010 09:16 PM
[NMR paper] Heteronuclear NMR assignments and secondary structure of the coiled coil trimerizatio Heteronuclear NMR assignments and secondary structure of the coiled coil trimerization domain from cartilage matrix protein in oxidized and reduced forms. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles Heteronuclear NMR assignments and secondary structure of the coiled coil trimerization domain from cartilage matrix protein in oxidized and reduced...	nmrlearner	Journal club	0	08-22-2010 05:08 PM
[NMR paper] Complete 1H, 13C, and 15N NMR resonance assignments and secondary structure of human Complete 1H, 13C, and 15N NMR resonance assignments and secondary structure of human glutaredoxin in the fully reduced form. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles Complete 1H, 13C, and 15N NMR resonance assignments and secondary structure of human glutaredoxin in the fully reduced form. Protein Sci. 1997 Feb;6(2):383-90 ...	nmrlearner	Journal club	0	08-22-2010 03:31 PM
[NMR paper] Secondary structure of Src homology 2 domain of c-Abl by heteronuclear NMR spectrosco Secondary structure of Src homology 2 domain of c-Abl by heteronuclear NMR spectroscopy in solution. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles Secondary structure of Src homology 2 domain of c-Abl by heteronuclear NMR spectroscopy in solution. Proc Natl Acad Sci U S A. 1992 Dec 15;89(24):11673-7 Authors: Overduin M, Mayer B, Rios CB, Baltimore D, Cowburn D The Src homology 2 (SH2) domain is a recognition motif thought to mediate the association of the...	nmrlearner	Journal club	0	08-21-2010 11:45 PM
[NMR paper] 1H, 13C, and 15N NMR backbone assignments and secondary structure of human interferon 1H, 13C, and 15N NMR backbone assignments and secondary structure of human interferon-gamma. Related Articles 1H, 13C, and 15N NMR backbone assignments and secondary structure of human interferon-gamma. Biochemistry. 1992 Sep 8;31(35):8180-90 Authors: Grzesiek S, DÃ¶beli H, Gentz R, Garotta G, Labhardt AM, Bax A 1H, 13C, and 15N NMR assignments of the protein backbone of human interferon-gamma, a homodimer of 31.4 kDa, have been made using the recently introduced three-dimensional (3D) triple-resonance NMR techniques. It is shown that, despite...	nmrlearner	Journal club	0	08-21-2010 11:45 PM
[NMR paper] Secondary structure and topology of interleukin-1 receptor antagonist protein determi Secondary structure and topology of interleukin-1 receptor antagonist protein determined by heteronuclear three-dimensional NMR spectroscopy. Related Articles Secondary structure and topology of interleukin-1 receptor antagonist protein determined by heteronuclear three-dimensional NMR spectroscopy. Biochemistry. 1992 Jun 16;31(23):5237-45 Authors: Stockman BJ, Scahill TA, Roy M, Ulrich EL, Strakalaitis NA, Brunner DP, Yem AW, Deibel MR Interleukin-1 (IL-1) proteins, such as IL-1 beta, play a key role in immune and inflammatory responses....	nmrlearner	Journal club	0	08-21-2010 11:41 PM
[NMR paper] Four-dimensional heteronuclear triple-resonance NMR spectroscopy of interleukin-1 bet Four-dimensional heteronuclear triple-resonance NMR spectroscopy of interleukin-1 beta in solution. Related Articles Four-dimensional heteronuclear triple-resonance NMR spectroscopy of interleukin-1 beta in solution. Science. 1990 Jul 27;249(4967):411-4 Authors: Kay LE, Clore GM, Bax A, Gronenborn AM A method is presented that dramatically improves the resolution of protein nuclear magnetic resonance (NMR) spectra by increasing their dimensionality to four. The power of this technique is demonstrated by the application of four-dimensional...	nmrlearner	Journal club	0	08-21-2010 11:04 PM
[NMR paper] Determination of the secondary structure and molecular topology of interleukin-1 beta Determination of the secondary structure and molecular topology of interleukin-1 beta by use of two- and three-dimensional heteronuclear 15N-1H NMR spectroscopy. Related Articles Determination of the secondary structure and molecular topology of interleukin-1 beta by use of two- and three-dimensional heteronuclear 15N-1H NMR spectroscopy. Biochemistry. 1990 May 15;29(19):4668-82 Authors: Driscoll PC, Gronenborn AM, Wingfield PT, Clore GM A study of the regular secondary structure elements of recombinant human interleukin-1 beta has been...	nmrlearner	Journal club	0	08-21-2010 10:48 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off