BACKGROUND: Symmetrical NMR spectroscopy, such as Total Correlation Spectroscopy (TOCSY) and other homonuclear spectroscopy, displays symmetry in chemical shift but are generally not symmetrical in terms of intensity, which constitutes a pivotal branch of multidimensional NMR spectroscopy and offers a robust tool for elucidating the structures and dynamics of complex samples, particularly in the context of biological macromolecules. Non-Uniform Sampling (NUS) stands as a critical technique for...
Reducing the measurement time of exact NOEs by non-uniform sampling
Reducing the measurement time of exact NOEs by non-uniform sampling
Abstract
We have previously reported on the measurement of exact NOEs (eNOEs), which yield a wealth of additional information in comparison to conventional NOEs. We have used these eNOEs in a variety of applications, including calculating high-resolution structures of proteins and RNA molecules. The collection of eNOEs is challenging, however, due to the need to measure a NOESY buildup series consisting of typically four NOESY spectra with varying mixing times in a single measurement...
[U. of Ottawa NMR Facility Blog] Non-uniform Sampling (NUS)
Non-uniform Sampling (NUS)
Collecting 2D or 3D NMR data can be very time consuming. The indirect dimension of a 2D experiment is sampled linearly via the t1 increments in the pulse sequence. An FID must be collected for every single linearly spaced t1 increment. In the interest in collecting 2D or 3D NMR data in a more time efficient manner, a great deal of effort is made towards faster data collection techniques. While some of these methods are based on spatial selectivity, others are based on sparse sampling techniques in the indirect dimensions of nD NMR sequences. One such sparse...
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05-11-2016 08:04 PM
[NMR paper] Non-uniform sampling of NMR relaxation data.
Non-uniform sampling of NMR relaxation data.
Related Articles Non-uniform sampling of NMR relaxation data.
J Biomol NMR. 2016 Feb 4;
Authors: Linnet TE, Teilum K
Abstract
The use of non-uniform sampling of NMR spectra may give significant reductions in the data acquisition time. For quantitative experiments such as the measurement of spin relaxation rates, non-uniform sampling is however not widely used as inaccuracies in peak intensities may lead to errors in the extracted dynamic parameters. By systematic reducing the coverage...
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02-06-2016 03:10 PM
Non-uniform sampling of NMR relaxation data
Non-uniform sampling of NMR relaxation data
Abstract
The use of non-uniform sampling of NMR spectra may give significant reductions in the data acquisition time. For quantitative experiments such as the measurement of spin relaxation rates, non-uniform sampling is however not widely used as inaccuracies in peak intensities may lead to errors in the extracted dynamic parameters. By systematic reducing the coverage of the Nyquist grid of 15N Carr-Purcell-Meiboom-Gill (CPMG) relaxation dispersion datasets for four different proteins and performing a full...
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02-05-2016 02:38 PM
[NMR paper] Non-Uniform Sampling and J-UNIO Automation for Efficient Protein NMR Structure Determination.
Non-Uniform Sampling and J-UNIO Automation for Efficient Protein NMR Structure Determination.
Related Articles Non-Uniform Sampling and J-UNIO Automation for Efficient Protein NMR Structure Determination.
Chemistry. 2015 Jul 28;
Authors: Didenko T, Proudfoot A, Dutta SK, Serrano P, Wüthrich K
Abstract
High-resolution structure determination of small proteins in solution is one of the big assets of NMR spectroscopy in structural biology. Improvements in the efficiency of NMR structure determination by advances in NMR experiments...
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08-02-2015 07:10 AM
[NMR paper] Time-resolved multidimensional NMR with non-uniform sampling.
Time-resolved multidimensional NMR with non-uniform sampling.
Time-resolved multidimensional NMR with non-uniform sampling.
J Biomol NMR. 2014 Jan 17;
Authors: Mayzel M, Rosenlöw J, Isaksson L, Orekhov VY
Abstract
Time-resolved experiments demand high resolution both in spectral dimensions and in time of the studied kinetic process. The latter requirement traditionally prohibits applications of the multidimensional experiments, which, although capable of providing invaluable information about structure and dynamics and almost unlimited...
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01-18-2014 11:31 AM
[NMR analysis blog] Non Uniform Sampling (NUS) NMR Processing
Non Uniform Sampling (NUS) NMR Processing
Background
In the last few years, Non-Uniform Sampling (NUS) has emerged as a very powerful tool to significantly speed up the acquisition of multidimensional NMR experiments due to the fact that only a subset of the usual linearly sampled data in the Nyquist grid is measured.
Unfortunately, this fast acquisition modality introduces a new challenge as the normal Fourier Transform will fail and consequently, special processing techniques are required.
A number of sophisticated methods have been proposed for reconstructing sparsely sampled 2D...
SCREENES: Enhancing non-uniform sampling reconstruction for symmetrical NMR spectroscopy
Linear Mode
